Cytotoxic Effects and Antitumor Activity of Polysaccharides Isolated from the Fruiting Body of Ganoderma lucidum Basidial Mushroom

Cytotoxic Effects and Antitumor Activity of Polysaccharides Isolated from the Fruiting Body of Ganoderma lucidum Basidial Mushroom

The work was devoted to the isolation of polysaccharides from Ganoderma lucidum basidiomycete raw material collected in Uzbekistan and the study of their cytotoxic effect and antitumor activity. Water-soluble polysaccharides were isolated from the basidiomycete raw material in a yield of 15.16% by s...

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Bibliographic Details
Published in:Pharmaceutical chemistry journal Vol. 56; no. 8; pp. 1045 - 1048
Main Authors: Khalilova, G. A., Turaev, A. S., Mulkhitdinov, B. I., Khaitmetova, S. B., Normakhamatov, N. S.
Format: Journal Article
Language:English
Published: New York Springer US 01-11-2022
Springer
Subjects:
Analysis
Medicine
Organic Chemistry
Pharmacology/Toxicology
Pharmacy
Polysaccharides
Proteins
Raw materials
polysaccharides
isolation
antitumor activity
cytotoxicity
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Summary:The work was devoted to the isolation of polysaccharides from Ganoderma lucidum basidiomycete raw material collected in Uzbekistan and the study of their cytotoxic effect and antitumor activity. Water-soluble polysaccharides were isolated from the basidiomycete raw material in a yield of 15.16% by successive water extraction. The isolated polysaccharides were purified from proteins by the Savage method. The amount of carbohydrates (52.74%) was determined using the phenol-sulfuric acid method. An analysis of the elemental composition showed that the isolated polysaccharide contained 5.27% N, 54.30% C, and 7.79% H. It was established that the isolated polysaccharides did not produce direct cytotoxic effects on CML and HeLa cells in vitro . The antitumor activity of the polysaccharides was studied in vivo . It was found that the isolated polysaccharides at 25 mg/kg/day for 10 d inhibited Ehrlich adenocarcinoma solid tumor growth in mice by 59.34 vol% and 60.21 wt%.
ISSN:0091-150X
1573-9031
DOI:10.1007/s11094-022-02750-8