Large area periodic, systematically changing, multishape nanostructures by laser interference lithography and cell response to these topographies

Large area periodic, systematically changing, multishape nanostructures by laser interference lithography and cell response to these topographies

The fabrication details to form large area systematically changing multishape nanoscale structures on a chip by laser interference lithography (LIL) are described. The feasibility of fabricating different geometries including dots, ellipses, holes, and elliptical holes in both x- and y- directions o...

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Bibliographic Details
Published in:Journal of biomedical optics Vol. 18; no. 3; p. 035002
Main Authors: Ertorer, Erden, Vasefi, Fartash, Keshwah, Joel, Najiminaini, Mohamadreza, Halfpap, Christopher, Langbein, Uwe, Carson, Jeffrey J. L, Hamilton, Douglas W, Mittler, Silvia
Format: Journal Article
Language:English
Published: United States Society of Photo-Optical Instrumentation Engineers 01-03-2013
Subjects:
Animals
Cell Adhesion - drug effects
Cell Shape
Cells, Cultured
Glass
Image Processing, Computer-Assisted - methods
Interference
Interferometers
Lasers
Lithography
Microscopy, Fluorescence
Nanostructure
Nanostructures - chemistry
Nanostructures - ultrastructure
Optical Imaging - methods
Osteoblasts - cytology
Rats
Silicon substrates
Topography
Vinculin - chemistry
periodic nanostructures
osteoblasts
adhesion and spreading of cells
Lloyd's interferometer
laser interference lithography
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Summary:The fabrication details to form large area systematically changing multishape nanoscale structures on a chip by laser interference lithography (LIL) are described. The feasibility of fabricating different geometries including dots, ellipses, holes, and elliptical holes in both x- and y- directions on a single substrate is shown by implementing a Lloyd's interferometer. The fabricated structures at different substrate positions with respect to exposure time, exposure angle and associated light intensity profile are analyzed. Experimental details related to the fabrication of symmetric and biaxial periodic nanostructures on photoresist, silicon surfaces, and ion milled glass substrates are presented. Primary rat calvarial osteoblasts were grown on ion-milled glass substrates with nanotopography with a periodicity of 1200 nm. Fluorescent microscopy revealed that cells formed adhesions sites coincident with the nanotopography after 24 h of growth on the substrates. The results suggest that laser LIL is an easy and inexpensive method to fabricate systematically changing nanostructures for cell adhesion studies. The effect of the different periodicities and transition structures can be studied on a single substrate to reduce the number of samples significantly.
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ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.18.3.035002