Search Results - "Kerruth, Silke"
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Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses
Published in Proceedings of the National Academy of Sciences - PNAS (22-05-2018)“…Glutamatergic synapses display a rich repertoire of plasticity mechanisms on many different time scales, involving dynamic changes in the efficacy of…”
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Kinetic Mechanisms of Fast Glutamate Sensing by Fluorescent Protein Probes
Published in Biophysical journal (07-01-2020)“…We have developed probes based on the bacterial periplasmic glutamate/aspartate binding protein with either an endogenously fluorescent protein or a synthetic…”
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High-speed imaging of glutamate release with genetically encoded sensors
Published in Nature protocols (01-05-2019)“…The strength of an excitatory synapse depends on its ability to release glutamate and on the density of postsynaptic receptors. Genetically encoded glutamate…”
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The kinetic mechanisms of fast-decay red-fluorescent genetically encoded calcium indicators
Published in The Journal of biological chemistry (15-03-2019)“…Genetically encoded calcium indicators (GECIs) are useful reporters of cell-signaling, neuronal, and network activities. We have generated novel fast variants…”
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Ca2+-Dependent and -Independent Calmodulin Binding to the Cytoplasmic Loop of Gap Junction Connexins
Published in International journal of molecular sciences (19-02-2023)“…Ca2+/calmodulin (Ca2+/CaM) interaction with connexins (Cx) is well-established; however, the mechanistic basis of regulation of gap junction function by…”
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6
In-Situ Observation of Membrane Protein Folding during Cell-Free Expression
Published in PloS one (15-03-2016)“…Proper insertion, folding and assembly of functional proteins in biological membranes are key processes to warrant activity of a living cell. Here, we present…”
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Sequential conformational transitions and α-helical supercoiling regulate a sensor histidine kinase
Published in Nature communications (18-08-2017)“…Sensor histidine kinases are central to sensing in bacteria and in plants. They usually contain sensor, linker, and kinase modules and the structure of many of…”
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Aureochrome 1 illuminated: structural changes of a transcription factor probed by molecular spectroscopy
Published in PloS one (24-07-2014)“…Aureochrome 1 from Vaucheria frigida is a recently identified blue-light receptor that acts as a transcription factor. The protein comprises a photosensitive…”
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Resonance Raman and FTIR spectroscopic characterization of the closed and open states of channelrhodopsin-1
Published in FEBS letters (27-06-2014)“…•The retinal isomers in CaChR1 are quantified.•The P2380 state is accumulated to record the Raman spectrum of the conductive state.•The frequencies of the…”
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Characterization of the Blue–Light‐Activated Adenylyl Cyclase mPAC by Flash Photolysis and FTIR Spectroscopy
Published in Photochemistry and photobiology (01-05-2017)“…The recently discovered photo‐activated adenylyl cyclase (mPAC from Microcoleus chthonoplastes) is the first PAC that owes a light‐, oxygen‐ and…”
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11
Fast Decay Variants of Red Fluorescent Genetically-Encoded Calcium Indicators
Published in Biophysical journal (02-02-2018)Get full text
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12
Improvements of the Kinetics of Red Fluorescent Calcium Indicators
Published in Biophysical journal (03-02-2017)Get full text
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13
Ca[sup.2+]-Dependent and -Independent Calmodulin Binding to the Cytoplasmic Loop of Gap Junction Connexins
Published in International journal of molecular sciences (01-02-2023)“…Ca[sup.2+]/calmodulin (Ca[sup.2+]/CaM) interaction with connexins (Cx) is well-established; however, the mechanistic basis of regulation of gap junction…”
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14
Calmodulin Interaction with Gap Junction Intracellular Loop Peptides
Published in Biophysical journal (02-02-2018)Get full text
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15
Ca 2+ -Dependent and -Independent Calmodulin Binding to the Cytoplasmic Loop of Gap Junction Connexins
Published in International journal of molecular sciences (19-02-2023)“…Ca /calmodulin (Ca /CaM) interaction with connexins (Cx) is well-established; however, the mechanistic basis of regulation of gap junction function by Ca /CaM…”
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16
Spectroscopic Analysis of Channelrhodopsin and its Chromophore
Published in Biophysical journal (28-01-2014)Get full text
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Tracking Folding Events During the Cell-Free Expression of Bacteriorhodopsin into Nanodiscs by Seiras
Published in Biophysical journal (28-01-2014)Get full text
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18
New Fluorescent Calix Crown Ethers, Part II: Synthesis and Complex Formation in Solution and the Solid State
Published in European Journal of Organic Chemistry (01-11-2008)“…New luminescent 1‐aza‐15‐crown‐5 ethers bearing a coumarin unit were synthesised leading to the cavitand‐based calix crown ether 9. The solid‐state structures…”
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