Light‐assisted drying (LAD): A new process for producing an amorphous trehalose preservation matrix for the storage of biologics

Light‐assisted drying (LAD): A new process for producing an amorphous trehalose preservation matrix for the storage of biologics

The requirement for temperature‐controlled storage can be challenging and expensive for the transportation and storage of biologics. Light‐assisted drying (LAD) is a new processing technique to prepare biologics for storage in a trehalose amorphous solid matrix at ambient temperatures. Samples are i...

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Bibliographic Details
Published in:Engineering reports (Hoboken, N.J.) Vol. 6; no. 11
Main Authors: Furr, Daniel P., Tsegaye, Anteneh A., Kern, Madeline R., Olson, Gunnar, Ye, Tang, Zhang, Yong, Trammell, Susan R.
Format: Journal Article
Language:English
Published: Hoboken, USA John Wiley & Sons, Inc 01-11-2024
Wiley
Subjects:
Ambient temperature
amorphous solids
anhydrous preservation
Biological products
bioprocess engineering
Cold storage
Crystallization
Dehydration
Digital cameras
Drying
Humidity
Infrared lasers
Lasers
Light
Low temperature resistance
Moisture content
Moisture resistance
Photonics
Polarized light
polarized light imaging
Proteins
Raman spectroscopy
Temperature
Thermal imaging
Thermal resistance
thermal stabilization
Titration
Trehalose
Water
Water distribution
Water engineering
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Summary:The requirement for temperature‐controlled storage can be challenging and expensive for the transportation and storage of biologics. Light‐assisted drying (LAD) is a new processing technique to prepare biologics for storage in a trehalose amorphous solid matrix at ambient temperatures. Samples are illuminated with near‐infrared laser light to speed dehydration. Previous work has shown LAD can prepare small‐volume (40 μL) samples, but the feasibility of applying LAD to larger samples remains unexplored. Here, LAD is applied to large‐volume samples (250 μL). Samples of a trehalose solution with an embedded protein were LAD processed and stored for 1 month. The end moisture contents of samples were determined after processing and storage. Thermal histories were monitored to determine optimal drying times. The trehalose matrix was characterized using polarized light imaging and Raman spectroscopy. Karl‐Fischer (KF) titration was used to measure the water content. The end moisture contents and thermal histories show high repeatability for LAD processing. Polarized light imaging demonstrates that the trehalose matrix was resistant to crystallization. Raman spectroscopy indicates uniform water distribution and KF titration reveals a low average water content (2.5%). LAD can stabilize large‐volume samples for dry‐state storage at ambient temperatures and offers a potential solution for cold‐chain storage challenges. Many biological products (e.g., vaccines, protein‐based therapeutics, nanomedicine products) are temperature sensitive and require temperature‐controlled storage and distribution that is expensive and especially challenging in low‐resource settings. Light‐assisted drying (LAD) is a new processing technique to dehydrate biologics in preparation for long‐term dry‐state storage at near‐ambient temperatures to reduce or eliminate the need for cold storage strategies.
ISSN:2577-8196
2577-8196
DOI:10.1002/eng2.12889