Phytochemical composition of Caesalpinia crista extract as potential source for inhibiting cholinesterase and β-amyloid aggregation: Significance to Alzheimer's disease

Objective: To screen plant extract fractions and elucidate the components present in Caesalpinia crista (C. crista) leaves for cholinergic and anti-amyloidogenic activities for the treatment of Alzheimer's diseases.Methods: This work has been carried out to study the action of C. crista extract...

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Published in:	Asian Pacific journal of tropical biomedicine Vol. 8; no. 10; pp. 500 - 512
Main Authors:	Chethana, KolambeRajappa, Sasidhar, BalappaSomappa, Naika, Mahadeva, Keri, RangappaSangappa
Format:	Journal Article
Language:	English
Published:	Centre for Nano and Material Sciences, Jain University, Jain Global Campus, Kanakapura, Ramanagaram, Bangalore-562112, India%Chemical Sciences and Technology Division, CSIR-National Institute for Interdisciplinary, Science and Technology (CSIR-NIIST), Kerala, Trivandrum-695019, India%Applied Nutrition Division, Defence Food Research Laboratory, Mysore - 570011, India 01-10-2018 Wolters Kluwer Medknow Publications
Subjects:	acetylcholinesterase alzheimer′s disease butyrylcholinesterase caesalpinia crista β-amyloid β-Amyloid Acetylcholinesterase Butyrylcholinesterase Alzheimer's disease Caesalpinia crista
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Summary:	Objective: To screen plant extract fractions and elucidate the components present in Caesalpinia crista (C. crista) leaves for cholinergic and anti-amyloidogenic activities for the treatment of Alzheimer's diseases.Methods: This work has been carried out to study the action of C. crista extracts from nonpolar to polar solvents toward inhibition of oxidative stress, cholinergic and amyloidosis. The antioxidant activity was studied using DPPH total antioxidant assay; cholinergic assay by Ellman's method and anti-amyloidogenic assay by thioflavin-T fluorescence and transmission electron microscopy.Results: The quantification of polyphenols was carried out following C. crista methanolic extract (CCMeOH) HPLC fingerprinting, along with LC-MS and elucidated by MS LAMPS database. GC-MS of CCMeOH was screened for potential moieties.In vitro experimental results showed that the CCMeOH was potential extract that exhibited active inhibition of antioxidant property, cholinergic enzymes acetylcholinesterase and butyrylcholinesterase. For anti-amyloidogenic evaluations, among all the extracts, the CCMeOH was found to have the potential toward inhibiting the oligomers, fibrillation of Aβ42 with good defibrillation of amyloid cascading properties.Conclusions:These results are also supported by the presence of polyphenols as the active ingredients. Multi-potent target drug therapy is a promising option in treating the Alzheimer's diseases. Methanolic extract of C. crista shows potential activity against cholinergic enzymes, Aβ42 aggregation with antioxidant activity.
ISSN:	2221-1691
DOI:	10.4103/2221-1691.244159