Search Results - "Kempner, E S"

Effects of high-energy electrons and gamma rays directly on protein molecules by Kempner, E S

“…High-energy electrons and gamma rays ionize molecules at random along their trajectories. In each event, chemical bonds are ruptured, releasing radiolytic…”
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Ca(2+)-independent cytosolic phospholipase A2 from macrophage-like P388D1 cells. Isolation and characterization by Ackermann, E J, Kempner, E S, Dennis, E A

“…A novel form of an ATP-regulated, oligomeric, Ca(2+)-independent phospholipase A2 (iPLA2) has been purified from the cytosol of the murine macrophage-like cell…”
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Direct effects of ionizing radiation on macromolecules by Kempner, Ellis S

“…In the dry or frozen states, macromolecules are damaged directly by interactions with ionizing radiation. As γ-rays and high-energy electrons randomly ionize…”
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Movable lobes and flexible loops in proteins Structural deformations that control biochemical activity by Kempner, E.S.

“…Two classes of protein whose structure is modified by small ligands are reviewed. Proteins of one group contain two massive domains joined by a flexible link;…”
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Damage to proteins due to the direct action of ionizing radiation by Kempner, E S

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Direct Radiation Damage Is Confined to a Single Polypeptide in Rabbit Immunoglobulin G by Miller, J.H., Draper, L.R., Kempner, E.S.

“…Frozen rabbit immunoglobulin G was exposed to high-energy electrons. The surviving polypeptide subunits were determined and analyzed by radiation target…”
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Macromolecular Cross Section and Cellular Localization: Determination by Radiation Target Methods by Kempner, E.S.

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A radiation target method for size determination of supercoiled plasmid DNA by Anchordoquy, T.J., Molina, M.dC, Kempner, E.S.

“…Supercoiled DNA plasmids were exposed in the frozen state to high-energy electrons. Surviving supercoiled molecules were separated from their degradation…”
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Novel predictions from radiation target analysis by Kempner, E S

“…The unusual technique of radiation inactivation has been used to determine the mass of many different macromolecules. Most of the radiation target sizes…”
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Radiation target analyses of free and immobilized glucose 6-phosphate dehydrogenase by Kempner, E.S., Miller, J.H.

“…The sensitivity of the enzyme glucose 6-phosphate dehydrogenase to ionizing radiation was examined under several conditions, including the presence of several…”
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A physiological role for titin and nebulin in skeletal muscle by Horowits, Robert, Kempner, Ellis S, Bisher, Margaret E, Podolsky, Richard J

“…Production of active force in skeletal muscle results from the interaction of myosin-containing thick filaments with actin-containing thin filaments. These…”
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A monomeric protein in the Golgi membrane catalyzes both N-deacetylation and N-sulfation of heparan sulfate by MANDON, E, KEMPNER, E. S, ISHIHARA, M, HIRSCHBERG, C. B

“…Recent studies have shown that the rat liver heparan sulfate N-deacetylase/N-sulfotransferase is a glycoprotein encoded by a single polypeptide chain of 882…”
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Effect of environmental conditions on radiation target size analyses by Kempner, E S, Miller, J H

“…Target size determinations from radiation inactivation of proteins is dependent on the physical and chemical environment of the sample during radiation…”
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Radiation inactivation analysis of acyl-CoA:retinol acyltransferase and lecithin:retinol acyltransferase in rat liver by ROSS, A. C, KEMPNER, E. S

“…Microsomes from liver and several other tissues esterify retinol through both fatty acyl-CoA-dependent and -independent reactions. Two activities,…”
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Oligomeric structure of p21 ras proteins as determined by radiation inactivation by Santos, E, Nebreda, A R, Bryan, T, Kempner, E S

“…Using radiation inactivation we determined that p21 ras proteins exhibit an oligomeric target size when assayed both structurally and functionally. Similar…”
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Radiation Effects on the Native Structure of Proteins: Fragmentation without Dissociation by Miller, J.H., Fedoronko, D.A., Hass, B.D., Myint, M., Kempner, E.S.

“…Several proteins (avidin, carboxypeptidase B, glucose-6-phosphate dehydrogenase, glutamate dehydrogenase, maltase, and peroxidase) composed of one to six…”
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The functional size of ferrochelatase determined in situ by radiation inactivation by STRAKA, J. G, BLOOMER, J. R, KEMPNER, E. S

“…Ferrochelatase (EC 4.99.1.1) catalyzes the final step of heme biosynthesis, the insertion of iron(II) into protoporphyrin. It is an integral protein of the…”
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Radiation inactivation of binding sites for high density lipoproteins in human fibroblast membranes by Mendel, C M, Kunitake, S T, Kane, J P, Kempner, E S

“…Radiation inactivation and target analysis were used to determine the molecular mass of the binding sites for high density lipoproteins (HDL) on membranes…”
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The functional and physical form of mammalian cytochrome c oxidase determined by gel filtration, radiation inactivation, and sedimentation equilibrium analysis by Suarez, M D, Revzin, A, Narlock, R, Kempner, E S, Thompson, D A, Ferguson-Miller, S

“…When solubilized in laurylmaltoside, cytochrome oxidases from beef heart and rat liver mitochondria exist as monodisperse populations that are stable, highly…”
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The influence of low temperature on the radiation sensitivity of enzymes by Kempner, E S, Haigler, H T

“…When enzymes are exposed to ionizing radiation at low temperatures there is a progressive decrease in radiation sensitivity: considerably more enzymatic…”
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