Search Results - "Kelley, Brian D"

Weak partitioning chromatography for anion exchange purification of monoclonal antibodies by D. Kelley, Brian, Tobler, Scott A., Brown, Paul, Coffman, Jonathan L., Godavarti, Ranga, Iskra, Timothy, Switzer, Mary, Vunnum, Suresh

“…Weak partitioning chromatography (WPC) is an isocratic chromatographic protein separation method performed under mobile phase conditions where a significant…”
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Exploration of overloaded cation exchange chromatography for monoclonal antibody purification by Liu, Hui F., McCooey, Beth, Duarte, Tiago, Myers, Deanna E., Hudson, Terry, Amanullah, Ashraf, van Reis, Robert, Kelley, Brian D.

“…Cation exchange chromatography using conventional resins, having either diffusive or perfusive flow paths, operated in bind-elute mode has been commonly…”
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High-throughput screening of chromatographic separations: I. Method development and column modeling by Coffman, Jonathan L, Kramarczyk, Jack F, Kelley, Brian D

“…The development of purification processes for protein biopharmaceuticals is challenging due to compressed development timelines, long experimental times, and…”
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The rapid identification of elution conditions for therapeutic antibodies from cation-exchange chromatography resins using high-throughput screening by McDonald, Paul, Tran, Benjamin, Williams, Christopher R., Wong, Marc, Zhao, Ti, Kelley, Brian D., Lester, Philip

“…•We developed a high-throughput cation-exchange chromatography screen.•We mapped the binding behavior of antibodies and impurities with this screen.•We found a…”
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High-throughput screening of chromatographic separations: II. Hydrophobic interaction by Kramarczyk, Jack F, Kelley, Brian D, Coffman, Jonathan L

“…A high-throughput screen (HTS) was developed to evaluate the selectivity of various hydrophobic interaction chromatography (HIC) resins for separating a mAb…”
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High-throughput screening of chromatographic separations: IV. Ion-exchange by Kelley, Brian D, Switzer, Mary, Bastek, Patrick, Kramarczyk, Jack F, Molnar, Kathleen, Yu, Tianning, Coffman, Jon

“…Ion-exchange (IEX) chromatography steps are widely applied in protein purification processes because of their high capacity, selectivity, robust operation, and…”
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High-throughput screening of chromatographic separations: III. Monoclonal antibodies on ceramic hydroxyapatite by Wensel, David L, Kelley, Brian D, Coffman, Jon L

“…High-throughput screening (HTS) of chromatography resins for identifying optimal protein purification conditions is becoming an integral part of industrial…”
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Effect of protein and solution properties on the donnan effect during the ultrafiltration of proteins by Bolton, Glen R., Boesch, Austin W., Basha, Jonida, LaCasse, Daniel P., Kelley, Brian D., Acharya, Hari

“…Formulation of protein biopharmaceuticals as highly concentrated liquids can improve the drug substance storage and supply chain, improve the target product…”
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Bioprocessing of therapeutic proteins by Kelley, Brian D

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Development and validation of an affinity chromatography step using a peptide ligand for cGMP production of factor VIII by Kelley, Brian D., Tannatt, Molly, Magnusson, Robert, Hagelberg, Sigrid, Booth, James

“…An affinity chromatography step was developed for purification of recombinant B‐Domain Deleted Factor VIII (BDDrFVIII) using a peptide ligand selected from a…”
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Quantitative Comparison of Global Carbohydrate Structures of Glycoproteins Using LC−MS and In-Source Fragmentation by Mazsaroff, Istvan, Yu, Wen, Kelley, Brian D, Vath, James E

“…A comparative method for the quantitative analysis of the ratio of oxonium fragment (reporter) ions derived from sialic acid and N-acetylhexosamine residues on…”
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Viral clearance studies on new and used chromatography resins: Critical review of a large dataset by Kelley, Brian D., Jakubik, Jocelyn, Vicik, Steven

“…Viral clearance studies for naïve and maximally cycled chromatographic resins used for cGMP recombinant protein production are reviewed for three products,…”
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Isolation of a peptide ligand for affinity purification of factor VIII using phage display by Kelley, Brian D., Booth, James, Tannatt, Molly, Wu, Qi-Long, Ladner, Robert, Yu, Jinan, Potter, Daniel, Ley, Arthur

“…Polypeptides for use in affinity chromatography of factor VIII were identified using phage display technology. Phage libraries were designed to express…”
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Effect of Inclusion Body Contaminants on the Oxidative Renaturation of Hen Egg White Lysozyme by Maachupalli-Reddy, Jhansi, Kelley, Brian D., Clark, Eliana De Bernardez

“…The effect of typical contaminants in inclusion body preparations such as DNA, ribosomal RNA, phospholipids, lipopolysaccharides, and other proteins on…”
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Characteristics and N-terminal amino acid sequence of a manganese peroxidase purified from Lentinula edodes cultures grown on a commercial wood substrate by Forrester, I.T, Grabski, A.C, Mishra, C, Kelley, B.D, Strickland, W.N, Leatham, G.F, Burgess, R.R

“…Extracellular culture filtrates from ligninolytic cultures of the lignin-degrading basidiomycete Lentinula (syn. Lentinus) edodes (Berk.) Pegler contained one…”
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Development and validation of an affinity chromatography step using a peptide ligand for cGMP production of factor VIII: Recovery and Purification of Biologics by KELLEY, Brian D, TANNATT, Molly, MAGNUSSON, Robert, HAGELBERG, Sigrid, BOOTH, James

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West Nile Virus inactivation by the solvent/detergent steps of the second and third generation manufacturing processes for B-domain deleted recombinant factor VIII by Jakubik, J. J., Vicik, S. M., Tannatt, M. M., Kelley, B. D.

“…West Nile Virus (WNV) was immediately and completely inactivated to below assay detection limits upon addition of solvent/detergent (S/D) to intermediate…”
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Regenerative Peripheral Nerve Interfaces for the Management of Symptomatic Hand and Digital Neuromas by Hooper, Rachel C, Cederna, Paul S, Brown, David L, Haase, Steven C, Waljee, Jennifer F, Egeland, Brent M, Kelley, Brian P, Kung, Theodore A

“…Painful neuromas result from traumatic injuries of the hand and digits and cause substantial physical disability, psychological distress, and decreased quality…”
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Management of Acute Postoperative Pain in Hand Surgery: A Systematic Review by Kelley, Brian P., MD, Shauver, Melissa J., MPH, Chung, Kevin C., MD, MS

“…Purpose To conduct a systematic review to guide hand surgeons in an evidenced-based approach in managing postoperative pain. Methods We performed a literature…”
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Soft-Tissue Coverage for Elbow Trauma by Kelley, Brian P., MD, Chung, Kevin C., MD, MS

“…The elbow is particularly prone to trauma, and soft-tissue reconstruction can be challenging given the inherent motion, pressure, and lack of local tissue…”
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