Search Results - "Keith, Charles H"

Coherent backscatter enhances reflection confocal microscopy by Keith, C H, Bird, G J, Farmer, M A

“…When thin optically transparent specimens are grown on reflective substrates, contrast in reflection confocal microscopy is markedly enhanced. This enhanced…”
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New statistical methods enhance imaging of cameleon fluorescence resonance energy transfer in cultured zebrafish spinal neurons by Fan, Xiang, Majumder, Anirban, Reagin, Sean S, Porter, Erika L, Sornborger, Andrew T, Keith, Charles H, Lauderdale, James D

“…Cameleons are genetically encoded fluorescence resonance energy transfer (FRET)-based Ca(2+) indicators. Attempts to use cameleons to detect neural activity in…”
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Micro-perfusion flow cell for imaging cultured cells by Agnihotri, N, Kisaalita, W S, Keith, C H

“…We present a unique design for a flow cell with a small working volume that allows rapid displacement of media viewed under high power and short working…”
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Visualization of the microtubules of glutaraldehyde-fixed cells by reflection-enhanced backscatter confocal microscopy by Keith, Charles H, Farmer, Mark A

“…Performing reflection-mode (backscatter-mode) confocal microscopy on cells growing on reflective substrates gives images that have improved contrast and are…”
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Preferential initiation of PC12 neurites in directions of changing substrate adhesivity by Murnane, Aeneas C., Brown, Kimberly, Keith, Charles H.

“…When PC12 cells are grown on substrates showing a gradient of nonspecific adhesion, they preferentially initiate neurites in directions of changing adhesivity,…”
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The Neurospora crassa Genome: Cosmid Libraries Sorted by Chromosome by Kelkar, Hemant S, Griffith, James, Case, Mary E, Covert, Sarah F, Hall, Robert D, Keith, Charles H, Oliver, J. Steve, Orbach, Marc J, Sachs, Matthew S, Wagner, Jeff R, Weise, Michael J, Wunderlich, John K, Arnold, Jonathan

“…A Neurospora crassa cosmid library of 12,000 clones (at least nine genome equivalents) has been created using an improved cosmid vector pLorist6Xh, which…”
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Local cytoplasmic calcium gradients in living mitotic cells by Keith, Charles H, Ratan, Rajiv, Maxfield, Frederick R, Bajer, Andrew, Shelanski, Michael L

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Slow Transport of Tubulin in the Neurites of Differentiated PC12 Cells by Keith, Charles H.

“…In order to study the rate and form of tubulin transport in cultured neuronal cells, the fluorescence recovery after the photobleaching of a fluorescent…”
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Glutamate‐induced changes in the pattern of hippocampal dendrite outgrowth: A role for calcium‐dependent pathways and the microtubule cytoskeleton by Wilson, Mark T., Kisaalita, William S., Keith, Charles H.

“…Glutamate regulation of a variety of aspects of dendrite development may be involved in neuronal plasticity and neuropathology. In this study, we examine the…”
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Nitrogen Oxides in Tobacco Smoke by NORMAN, VELLO, KEITH, CHARLES H

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Neurite elongation is blocked if microtubule polymerization is inhibited in PC12 cells by Keith, C H

“…We have injected process-bearing PC12 cells with colchicine-tubulin mixed with either fluorescein-dextran or a rhodamine-labelled tubulin analogue to determine…”
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Glutamate modulation of dendrite outgrowth: Alterations in the distribution of dendritic microtubules by Wilson, Mark T., Keith, Charles H.

“…Glutamate can both facilitate and inhibit dendrite outgrowth in vitro. The major effects of low levels of glutamate occur only on the dendrites (not the axon)…”
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Free cyclic AMP increases in PC12 cells on depolarization by Agnihotri, Naveen, Kisaalita, William S., Keith, Charles H.

“…The temporal dynamics of the intracellular second messenger cyclic AMP (cAMP) were monitored in living PC12 cells by digital fluorescence ratio imaging using…”
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Quantitative fluorescence techniques for the determination of local microtubule polymerization equilibria in cultured neurons by Keith, C H

“…The local control of intracellular microtubule polymerization equilibria has been hypothesized to be an important factor in the determination of neurite…”
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Rapid Acidification of Endocytic Vesicles Containing Asialoglycoprotein in Cells of a Human Hepatoma Line by Tycko, Benjamin, Keith, Charles H., Maxfield, Frederick R.

“…Acidification of endocytic vesicles has been implicated as a necessary step in various processes including receptor recycling, virus penetration, and the entry…”
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Thyrotropin-Releasing Hormone-Induced Changes in Intracellular [Ca2+] Measured by Microspectrofluorometry on. Individual Quin2-Loaded Cells by Kruskal, Benjamin A., Keith, Charles H., Maxfield, Frederick R.

“…We have developed an accurate and practical method for measuring intracellular Ca2+concentration ([Ca2+]i) in single cells in monolayer culture using the…”
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Intracellular Free Calcium Levels are Reduced in Mitotic Pt K2 Epithelial Cells by Keith, Charles H., Maxfield, Frederick R., Shelanski, Michael L.

“…Using a fluorescence ratio method, we have studied the intracellular free calcium levels in individual quin-2-loaded mitotic cells under the microscope. We…”
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Trypanosoma cruzi infection of BSC-1 fibroblast cells causes cytoskeletal disruption and changes in intracellular calcium levels by Low, H P, Paulin, J J, Keith, C H

“…The disruption of vimentin and actin filaments of host BSC-1 fibroblast cells by Trypanosoma cruzi was investigated using a mouse monoclonal anti-vimentin…”
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Intracellular pH of tissue-cultured bovine corneal endothelial cells by Chu, T C, Keith, C H, Yee, E, Green, K

“…Intracellular pH (pHi) of bovine tissue-cultured corneal endothelial cells has been measured under several experimental conditions. Determinations were made on…”
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Direct Visualization of Fluorescein-Labeled Microtubules in vitro and in Microinjected Fibroblasts by Keith, Charles H., Feramisco, James R., Shelanski, Michael

“…Microtubule proteins and tubulin have been purified from brain and labeled with dichlorotriazinyl fluorescein (DTAF). This procedure compromises neither the…”
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