DNA-membrane complex formation during electroporation is DNA size-dependent

DNA-membrane complex formation during electroporation is DNA size-dependent

Size of DNA molecules governs their interaction with the cell membrane during electroporation and their subsequent transport inside the cell. In order to investigate the effect of DNA size on DNA-membrane interaction during electroporation, cells are electro-pulsed with DNA molecules; 15 bp, 25 bp,...

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Bibliographic Details
Published in:Biochimica et biophysica acta. Biomembranes Vol. 1862; no. 2; p. 183089
Main Authors: Sachdev, Shaurya, Feijoo Moreira, Sara, Keehnen, Yasmine, Rems, Lea, Kreutzer, Michiel T., Boukany, Pouyan E.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-02-2020
Subjects:
Cell Membrane - metabolism
Cell Membrane Permeability
Cytoplasm - metabolism
DNA - metabolism
DNA aggregation
DNA size
DNA-membrane complex formation
Electropermeabilization
Electroporation
Electroporation - methods
Gene electrotransfer
Gene Transfer Techniques
Particle Size
99-00
DNA size
DNA aggregation
00-01
Electropermeabilization
Gene electrotransfer
Electroporation
DNA-membrane complex formation
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Summary:Size of DNA molecules governs their interaction with the cell membrane during electroporation and their subsequent transport inside the cell. In order to investigate the effect of DNA size on DNA-membrane interaction during electroporation, cells are electro-pulsed with DNA molecules; 15 bp, 25 bp, 50 bp, 100 bp and 1000 bp (bp = base pairs). Within the experimental parameter space, DNA-membrane complexes or DNA aggregates are observed at the cell membrane for DNA molecules containing 25 or more base pairs. No aggregates are observed for DNA molecules containing 15 bp. For all DNA sizes, direct access to the cytoplasm is observed, however the amount translocated decays with the size. The observed dependency of DNA aggregate formation on the size of the DNA molecules is consistent with the Onsager's theory of condensation of anisotropic rod-like molecules. [Display omitted] •DNA aggregate formation during electroporation is an onset phenomenon and occurs for even small DNA containing 25-100 bp•Aggregate formation is not observed for DNA molecules containing 15 bp.•The observed dependency is consistent with Onsager's theory of condensation of anisotropic rod-like molecules.•Direct access to the cytoplasm is observed for all DNA sizes, however, the amount translocated decays with the DNA size.
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ISSN:0005-2736
1879-2642
1879-2642
DOI:10.1016/j.bbamem.2019.183089