Search Results - "Ke, Yuqing"

Hairpin‐Spacer crRNA‐Enhanced CRISPR/Cas13a System Promotes the Specificity of Single Nucleotide Polymorphism (SNP) Identification by Ke, Yuqing, Huang, Shiyi, Ghalandari, Behafarid, Li, Sijie, Warden, Antony R., Dang, Jingqi, Kang, Lin, Zhang, Yu, Wang, Yunqing, Sun, Yiqing, Wang, Jinglin, Cui, Daxiang, Zhi, Xiao, Ding, Xianting

“…The Cas13a system has great potential in RNA interference and molecular diagnostic fields. However, lacking guidelines for crRNA design hinders practical…”
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One-Pot Visual Detection of African Swine Fever Virus Using CRISPR-Cas12a by Qin, Chao, Liu, Jiajia, Zhu, Wenqi, Zeng, Muchu, Xu, Ke, Ding, Jinmei, Zhou, Hao, Zhu, Jianshen, Ke, Yuqing, Li, Lai Yan, Sheng, Gaoyuan, Li, Zhuoru, Luo, Huaixi, Jiang, Shengyao, Chen, Kangchun, Ding, Xianting, Meng, He

“…African swine fever virus (ASFV) is a leading cause of worldwide agricultural loss. ASFV is a highly contagious and lethal disease for both domestic and wild…”
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Profiling extracellular vesicle surface proteins with 10 µL peripheral plasma within 4 h by He, Jie, Li, Hengyu, Mai, John, Ke, Yuqing, Zhai, Chunhui, Li, Jiao Jiao, Jiang, Lai, Shen, Guangxia, Ding, Xianting

“…Abstract Extracellular vesicle (EV) surface proteins, expressed by primary tumours, are important biomarkers for early cancer diagnosis. However, the detection…”
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Single-Cell Microwell Platform Reveals Circulating Neural Cells as a Clinical Indicator for Patients with Blood-Brain Barrier Breakdown by Zhang, Yu, Warden, Antony R., Ahmad, Khan Zara, Liu, Yanlei, He, Xijun, Zheng, Minqiao, Huo, Xinlong, Zhi, Xiao, Ke, Yuqing, Li, Hongxia, Yan, Sijia, Su, Wenqiong, Cai, Deng, Ding, Xianting

“…Central nervous system diseases commonly occur with the destruction of the blood-brain barrier. As a primary cause of morbidity and mortality, stroke remains…”
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A One-Pot Toolbox Based on Cas12a/crRNA Enables Rapid Foodborne Pathogen Detection at Attomolar Level by Wang, Yunqing, Ke, Yuqing, Liu, Wenjia, Sun, Yiqing, Ding, Xianting

“…Bacterial contamination accounts for more than half of food poisoning cases. Conventional methods such as colony-counting and general polymerase chain reaction…”
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Harnessing Multiplex crRNA in the CRISPR/Cas12a System Enables an Amplification-Free DNA Diagnostic Platform for ASFV Detection by Zeng, Muchu, Ke, Yuqing, Zhuang, Zhiyi, Qin, Chao, Li, Lai Yan, Sheng, Gaoyuan, Li, Zhuoru, Meng, He, Ding, Xianting

“…CRISPR-associated (Cas) protein systems have been increasingly incorporated in nucleic-acid diagnosis. CRISPR/Cas12a can cleave single-stranded DNA (ssDNA)…”
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Engineered CRISPRa System for Precise and Multiplex Gene Regulation Using a Hybrid dCas12a Variant and Hairpin-Spacer crRNAs by Ke, Yuqing, Zhang, Shuang, Gao, Yingying, Chen, Xiaoxiang, He, Jie, Chen, Youming, Zhang, Qiang, Ding, Xianting

“…Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a nucleases have emerged as a promising alternative to CRISPR-Cas9 in gene editing and…”
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2'- O -Methyl modified guide RNA promotes the single nucleotide polymorphism (SNP) discrimination ability of CRISPR-Cas12a systems by Ke, Yuqing, Ghalandari, Behafarid, Huang, Shiyi, Li, Sijie, Huang, Chengjie, Zhi, Xiao, Cui, Daxiang, Ding, Xianting

“…The CRISPR-Cas12a system has been widely applied to genome editing and molecular diagnostics. However, off-target cleavages and false-positive results remain…”
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One-pot pre-coated interface proximity extension assay for ultrasensitive co-detection of anti-SARS-CoV-2 antibodies and viral RNA by Yan, Sijia, Ahmad, Khan Zara, Warden, Antony R., Ke, Yuqing, Maboyi, Nokuzola, Zhi, Xiao, Ding, Xianting

“…In the field of in vitro diagnostics, detection of nucleic acids and proteins from biological samples is typically performed with independent platforms;…”
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New Structure Mass Tag based on Zr‐NMOF for Multiparameter and Sensitive Single‐Cell Interrogating in Mass Cytometry by Dang, Jingqi, Li, Hongxia, Zhang, Lulu, Li, Sijie, Zhang, Ting, Huang, Shiyi, Li, Yiyang, Huang, Chengjie, Ke, Yuqing, Shen, Guangxia, Zhi, Xiao, Ding, Xianting

“…Mass cytometry, also called cytometry by time‐of‐flight (CyTOF), is an emerging powerful proteomic analysis technique that utilizes metal chelated polymer…”
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A HiPAD Integrated with rGO/MWCNTs Nano‐Circuit Heater for Visual Point‐of‐Care Testing of SARS‐CoV‐2 by Li, Sijie, Huang, Shiyi, Ke, Yuqing, Chen, Hongjun, Dang, Jingqi, Huang, Chengjie, Liu, Wenjia, Cui, Daxiang, Wang, Jinglin, Zhi, Xiao, Ding, Xianting

“…Nowadays, the main obstacle for further miniaturization and integration of nucleic acids point‐of‐care testing devices is the lack of low‐cost and…”
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CRISPR/Cas12a Powered DNA Framework‐Supported Electrochemical Biosensing Platform for Ultrasensitive Nucleic Acid Analysis by Su, Jing, Ke, Yuqing, Maboyi, Nokuzola, Zhi, Xiao, Yan, Sijia, Li, Fuwu, Zhao, Bo, Jia, Xiaolong, Song, Shiping, Ding, Xianting

“…Nucleic acid analysis using ultrasensitive and simple methods is critically important for the early‐stage diagnosis and treatment of diseases. The CRISPR/Cas…”
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Encountering and Wrestling: Neutrophils Recognize and Defensively Degrade Graphene Oxide by Huang, Shiyi, Li, Sijie, Liu, Yanlei, Ghalandari, Behafarid, Hao, Ling, Huang, Chengjie, Su, Wenqiong, Ke, Yuqing, Cui, Daxiang, Zhi, Xiao, Ding, Xianting

“…The boosting exploitation of graphene oxide (GO) increases exposure risk to human beings. However, as primary defender in the first immune line, neutrophils’…”
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CRISPR/Cas12a Powered DNA Framework‐Supported Electrochemical Biosensing Platform for Ultrasensitive Nucleic Acid Analysis (Small Methods 12/2021) by Su, Jing, Ke, Yuqing, Maboyi, Nokuzola, Zhi, Xiao, Yan, Sijia, Li, Fuwu, Zhao, Bo, Jia, Xiaolong, Song, Shiping, Ding, Xianting

“…Front Cover In article number 2100935, Song, Ding, and co‐workers have developed a CRISPR/Cas12a powered DNA framework‐supported biosensing platform via…”
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Inhomogeneity of microstructure and mechanical properties in the nugget of friction stir welded thick 7075 aluminum alloy joints by Mao, Yuqing, Ke, Liming, Chen, Yuhua, Liu, Fencheng, Xing, Li

“…In this study, 20 mm thick AA7075-T6 alloy plates were joined by friction stir welding. The microstructure and mechanical properties of the nugget zone along…”
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