Search Results - "Kayastha, Arvind M"
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Thermal, chemical and pH induced denaturation of a multimeric β-galactosidase reveals multiple unfolding pathways
Published in PloS one (21-11-2012)“…In this case study, we analysed the properties of unfolded states and pathways leading to complete denaturation of a multimeric chick pea β-galactosidase…”
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2
Immobilization of β-galactosidase onto functionalized graphene nano-sheets using response surface methodology and its analytical applications
Published in PloS one (18-07-2012)“…β-Galactosidase is a vital enzyme with diverse application in molecular biology and industries. It was covalently attached onto functionalized graphene…”
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3
Immobilization of soybean ( Glycine max) urease on alginate and chitosan beads showing improved stability: Analytical applications
Published in Journal of molecular catalysis. B, Enzymatic (01-06-2009)“…The soybean ( Glycine max) urease was immobilized on alginate and chitosan beads and various parameters were optimized and compared. The best immobilization…”
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4
Heat, Acid and Chemically Induced Unfolding Pathways, Conformational Stability and Structure-Function Relationship in Wheat α-Amylase
Published in PloS one (08-06-2015)“…Wheat α-amylase, a multi-domain protein with immense industrial applications, belongs to α+β class of proteins with native molecular mass of 32 kDa. In the…”
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5
In Silico Structural and Functional Insight into the Binding Interactions of the Modeled Structure of Watermelon Urease with Urea
Published in ACS omega (16-01-2024)“…Urease (EC 3.5.1.5) is an amidohydrolase. This nickel-dependent metalloenzyme converts urea into NH3 and CO2. Despite their vital role in plants, the structure…”
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6
Functionalized graphene sheets as immobilization matrix for Fenugreek β-amylase: enzyme kinetics and stability studies
Published in PloS one (2014)“…β-Amylase finds application in food and pharmaceutical industries. Functionalized graphene sheets were customised as a matrix for covalent immobilization of…”
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7
Biophysical Investigation of the Interplay between the Conformational Species of Domain-Swapped GB1 Amyloid Mutant through Real–Time Monitoring of Amyloid Fibrillation
Published in ACS omega (21-12-2021)“…Mutant polypeptide GB1HS#124F26A, which is known to aggregate into amyloid-like fibrils, has been utilized as a model in this study for gaining insights into…”
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8
Β-amylase from starchless seeds of Trigonella foenum-graecum and its localization in germinating seeds
Published in PloS one (2014)“…Fenugreek (Trigonella foenum-graecum) seeds do not contain starch as carbohydrate reserve. Synthesis of starch is initiated after germination. A β-amylase from…”
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9
Denaturant Induced Equilibrium Unfolding and Conformational Transitional Studies of Germinated Fenugreek β-Amylase Revealed Molten Globule like State at Low pH
Published in Protein and peptide letters (01-01-2020)“…β-Amylase (EC 3.2.1.2) is a maltogenic enzyme, which releases β-maltose from the non-reducing end of the substrates. The enzyme plays important roles for the…”
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Correction: Heat, Acid and Chemically Induced Unfolding Pathways, Conformational Stability and Structure-Function Relationship in Wheat α-Amylase
Published in PloS one (2015)“…[This corrects the article DOI: 10.1371/journal.pone.0129203.]…”
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11
Enzymatic hydrolysis of native granular starches by a new β-amylase from peanut (Arachis hypogaea)
Published in Food chemistry (15-03-2019)“…•Glycogen precipitated β-amylase from peanut was used for native starch hydrolysis.•The process minimizes gelatinization step.•DE value obtained for corn and…”
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12
Exploring the catalytic potential of watermelon urease: Purification, biochemical characterization, and heavy metal precipitation
Published in International journal of biological macromolecules (01-12-2024)“…Bioactive urease from watermelon (Citrullus lanatus) seeds was purified using acetone fractionation, anion-exchange, and size-exclusion chromatography,…”
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13
Innovative synthesis approaches and health implications of organic-inorganic Nanohybrids for food industry applications
Published in Food chemistry (01-02-2025)“…Recent advancements in nanomaterials have significantly impacted various sectors, including medicine, energy, and manufacturing. Among these, organic/inorganic…”
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14
Enhancing milk quality assessment with watermelon (Citrullus lanatus) urease immobilized on VS2-chitosan nanocomposite beads using response surface methodology
Published in Food chemistry (01-09-2024)“…[Display omitted] •Synthesis of VS2 nanosheet using an eco-friendly hydrothermal approach.•BBD-based optimization resulting in Immobilization efficiency:…”
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15
Covalent immobilization of peanut β-amylase for producing industrial nano-biocatalysts: A comparative study of kinetics, stability and reusability of the immobilized enzyme
Published in Food chemistry (15-04-2018)“…•Covalent immobilization of β-amylase onto GO-CNT, GO and Fe3O4 was investigated.•Box-Behnken design was used to optimize parameters for maximum…”
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Immobilization of α-amylase onto functionalized molybdenum diselenide nanoflowers (MoSe2-NFs) as scaffolds: Characterization, kinetics, and potential applications in starch-based industries
Published in Food chemistry (01-06-2024)“…•Hydrothermal synthesis was used to create MoSe2-NFs easily and sustainably.•The investigation focused on the covalent immobilization of α-amylase onto…”
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17
Lactose nano-probe optimized using response surface methodology
Published in Biosensors & bioelectronics (15-12-2009)“…A lactose nano-probe has been developed by immobilization of PsBGAL onto gold nanoparticles (AuNps). It is helpful for severe lactose intolerants for quality…”
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18
Studies of histidine residues in soybean (Glycine max) urease
Published in Protein and peptide letters (01-06-2012)“…Soybean urease has been investigated extensively to reveal the presence of histidine residue (s) in the active site and their potential role in the catalysis…”
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α-Amylase purified and characterized from fenugreek (Trigonella foenum-graecum) showed substantial anti-biofilm activity against Staphylococcus aureus MTCC740
Published in International journal of biological macromolecules (01-12-2023)“…Starch hydrolyzing α-amylase from germinated fenugreek (Trigonella foenum-graecum) has been purified 104-fold to apparent electrophoretic homogeneity with a…”
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20
Studies on α-amylase inhibition by acarbose and quercetin using fluorescence, circular dichroism, docking, and dynamics simulations
Published in Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (05-06-2024)“…[Display omitted] •Fluorescence spectra were used to measure the interaction between α-amylase-ACA and α-amylase-QUE.•The circular dichroism spectra were used…”
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