Optimization of cell suspension culture of transformed and untransformed lettuce for the enhanced production of secondary metabolites and their pharmaceutical evaluation

In vitro suspension culture techniques are cost effective for large-scale production of secondary metabolites. In the present study, firstly, suspension cultures of untransformed Lactuca sativa were prepared using different hormonal combinations and were subjected to different pH, temperature and sa...

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Bibliographic Details
Published in:	3 Biotech Vol. 9; no. 9; pp. 339 - 17
Main Authors:	Ismail, Hammad, Kayani, Sania Sabahat, Kayani, Sadaf Ilyas, Mirza, Bushra, Waheed, Mohammad Tahir
Format:	Journal Article
Language:	English
Published:	Cham Springer International Publishing 01-09-2019 Springer Nature B.V
Subjects:	Agriculture Analgesics Anti-inflammatory agents Anticoagulants Antidepressants Antioxidants Assaying Bioinformatics Biomaterials Biotechnology Cancer Research Cell culture Chemistry Chemistry and Materials Science Clotting Culture techniques Exudates Exudation Flavonoids Inflammation Lettuce Metabolites Optimization Original Original Article pH effects Phenolic compounds Secondary metabolites Sodium chloride Stem Cells Suspension culture Weight Antioxidants Rol genes Rats Cell suspension Transgenic plants Lactuca sativa
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Summary:	In vitro suspension culture techniques are cost effective for large-scale production of secondary metabolites. In the present study, firstly, suspension cultures of untransformed Lactuca sativa were prepared using different hormonal combinations and were subjected to different pH, temperature and salt concentrations. Maximum biomass was obtained for suspensions supplemented with 1.5 mg/L BAP and 0.1 mg/L NAA, at pH 5.8, temperature 28 °C and 0 mM NaCl concentration. Using these parameters, suspensions were produced for rol ABC- and rol C -transformed lines of L. sativa . All the transgenic lines showed prominent increase in fresh weight (FW) and dry weight (DW) with maximum values for rol ABC 2 line producing 169.8 mg/mL FW and 25.3 mg/mL DW. The exudates of transformed and untransformed plants were tested for the antioxidant activity and in vivo assays on rats. Maximum phenolic content (261 μg/mL) and flavonoid content (637.6 μg/mL) were obtained for rol C 1 transgenic line. Total antioxidant capacity was found maximum (1451.7 μg/mL) for untransformed lettuce, whereas rol C 1 showed maximum total reducing power activity (637.6 μg/mL). In DPPH assay, maximum activity (104.7 μg/mL) was shown by rol ABC 3 line. In rats analgesic assay, maximum activity (74.9%) was shown by rol C 2. Line rol C 1 showed maximum anti-inflammatory activity (69.2%) and maximum antidepressant activity (minimum immobility time of 55 s). Maximum anticoagulant activity was observed for rol ABC 2 with maximum clotting time of 130 s. The present study could help in using lettuce suspension culture as platform for the enhanced production of important metabolites.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2190-572X 2190-5738
DOI:	10.1007/s13205-019-1870-x