Investigation of metallo-beta-lactamase producing strains of Pseudomonas aeruginosa and Acinetobacter baumannii by E-test, disk synergy and PCR

Carbapenem non-susceptible Pseudomonas aeruginosa and Acinetobacter baumannii strains were tested for the presence of metallo-beta-lactamases (MBLs) by EDTA-synergy screening. Imipenem hydrolysis was investigated by a bioassay and IMP-/VIM-encoding genes by PCR. No blaIMP/VIM related genes or imipen...

Full description

Saved in:

Bibliographic Details
Published in:	Scandinavian journal of infectious diseases Vol. 40; no. 4; pp. 320 - 325
Main Authors:	Akta, Zerrin, Kayacan, Çi dem Bal
Format:	Journal Article
Language:	English
Published:	Basingstoke Informa UK Ltd 2008 Taylor & Francis
Subjects:	Acinetobacter baumannii Acinetobacter baumannii - drug effects Acinetobacter baumannii - enzymology Acinetobacter baumannii - genetics Acinetobacter Infections - epidemiology Acinetobacter Infections - microbiology Adult Anti-Bacterial Agents - pharmacology beta-Lactamases - biosynthesis beta-Lactamases - genetics Biological and medical sciences Child Disk Diffusion Antimicrobial Tests Drug Resistance, Bacterial Edetic Acid - pharmacology Genotype Humans Imipenem - pharmacology Infectious diseases Medical sciences Microbial Sensitivity Tests - methods Phenotype Polymerase Chain Reaction - methods Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - enzymology Pseudomonas aeruginosa - genetics Pseudomonas Infections - epidemiology Pseudomonas Infections - microbiology Turkey - epidemiology Turkey Pseudomonadales Microbiology Enzyme Neisseriaceae β-Lactamase Infection Polymerase chain reaction Acinetobacter baumannii Bacteria Hydrolases Micrococcales Pseudomonadaceae Pseudomonas aeruginosa Molecular biology
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Carbapenem non-susceptible Pseudomonas aeruginosa and Acinetobacter baumannii strains were tested for the presence of metallo-beta-lactamases (MBLs) by EDTA-synergy screening. Imipenem hydrolysis was investigated by a bioassay and IMP-/VIM-encoding genes by PCR. No blaIMP/VIM related genes or imipenemase activity were detected although E-test found all strains as MBL-positive. Disk synergy tests with 0.5M EDTA determined 63.6-100%, while those with 0.1M EDTA detected 0-7.7% of isolates as MBL producers. Most strains were susceptible to EDTA. In conclusion, for MBL-screening purposes, EDTA-synergy results change with molarity of EDTA, but even if some false positives are encountered, 0.1M EDTA seems to be acceptable.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0036-5548 1651-1980
DOI:	10.1080/00365540701704698