Evaluation of Salmonella biofilm attachment and hydrophobicity characteristics on food contact surfaces

Evaluation of Salmonella biofilm attachment and hydrophobicity characteristics on food contact surfaces

Salmonella forms biofilms, and persist on food contact surfaces. Once a biofilm is formed cleaning and sanitation protocols may be inadequate for effective removal. This study evaluated attachment characteristics, surface properties, and structure of Salmonella biofilms on food contact surfaces comm...

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Bibliographic Details
Published in:BMC microbiology Vol. 24; no. 1; pp. 387 - 8
Main Authors: Ivers, Colton, Kaya, Eda C, Yucel, Umut, Boyle, Dan, Trinetta, Valentina
Format: Journal Article
Language:English
Published: England BioMed Central 03-10-2024
BMC
Subjects:
Attachment
Bacteria
Bacterial Adhesion
Biofilm
Biofilms
Biofilms - growth & development
Bioreactors
Confocal microscopy
Contact angle
Disease control
Experiments
Food
Food Contamination
Food industry
Food Microbiology
High density polyethylenes
Hydrophobic and Hydrophilic Interactions
Hydrophobicity
Lasers
Measuring instruments
Microscopy
Microscopy, Confocal
Pathogens
Polyethylene
Salmonella
Salmonella - growth & development
Salmonella - physiology
Sanitation
Surface Properties
United States > US
Biofilm
Salmonella
Attachment
Hydrophobicity
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Summary:Salmonella forms biofilms, and persist on food contact surfaces. Once a biofilm is formed cleaning and sanitation protocols may be inadequate for effective removal. This study evaluated attachment characteristics, surface properties, and structure of Salmonella biofilms on food contact surfaces commonly used in the tree-fruit industry. Multi-strain Salmonella biofilms were grown in a Centers for Disease Control and Prevention (CDC) biofilm reactor at 22 ± 2 °C and sampling was conducted at 2, 24 and 96-h. After each incubation period, coupons weregently rinsed and the remaining cells enumerated. Biofilms were analyzed with Laser Scanning Confocal Microscopy (LSCM). Hydrophobicity was evaluated by measuring the contact angles of reference liquids method using a drop tensiometer instrument. Material type and biofilm age significantly influenced attachment and biofilm hydrophobicity (P < 0.05). The strength of attachment, across all time points, was highest on nylon followed by wood and high-density polyethylene. The highest contact angle measurements were observed after 96-h of biofilm formation for each material. All the results and observations from this study contribute to a better understanding of the attachment and hydrophobicity characteristics of Salmonella and might help producers make informed decisions when selecting containers for harvesting and storing in order to minimize biofilm formation and potential for cross-contamination.
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ISSN:1471-2180
1471-2180
DOI:10.1186/s12866-024-03556-2