Characterisation of myofibroblasts in fibrovascular tissues of primary and recurrent pterygia

Aims: To determine the presence and origin of myofibroblasts in pterygia. Methods: 86 specimens including head, body, and fibrovascular tissue from 52 primary and 34 recurrent pterygia and five exenterated eyes without pterygia were searched for the origin of myofibroblasts. All tissues were subject...

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Bibliographic Details
Published in:	British journal of ophthalmology Vol. 89; no. 3; pp. 269 - 274
Main Authors:	Touhami, A, Di Pascuale, M A, Kawatika, T, Valle, M Del, Rosa, R H, Dubovy, S, Tseng, S C G
Format:	Journal Article
Language:	English
Published:	BMA House, Tavistock Square, London, WC1H 9JR BMJ Publishing Group Ltd 01-03-2005 BMJ BMJ Publishing Group LTD Copyright 2005 British Journal of Ophthalmology
Subjects:	3-amino-9-ethylcarbazole chromogen Actins - analysis Adipose Tissue - pathology Adult AEC Aged alpha smooth muscle actin Astigmatism Biological and medical sciences Biomarkers - analysis Calmodulin-Binding Proteins - analysis Clinical Science - Scientific Reports Desmin - analysis Diseases of eyelid, conjunctiva and lacrimal tracts Enzymes Female Fibroblasts Fibroblasts - pathology Fibrosis FITC fluorescein isothiocyanate Genotype & phenotype Growth factors Histology Humans Immunoglobulins Immunohistochemistry Male Medical sciences Microscopy, Electron Middle Aged Morphology Muscles - pathology myofibroblasts Ophthalmology Patients proliferation pterygium Pterygium - pathology Recurrence TEM transmission electron microscopy Vimentin - analysis α-SMA Pterygium Tissue Eye disease Relapse Myofibroblast Primary Conjunctiva disease Ophthalmology Recurrent
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Summary:	Aims: To determine the presence and origin of myofibroblasts in pterygia. Methods: 86 specimens including head, body, and fibrovascular tissue from 52 primary and 34 recurrent pterygia and five exenterated eyes without pterygia were searched for the origin of myofibroblasts. All tissues were subjected to haematoxylin and eosin staining, immunohistochemistry using antibodies against alpha smooth muscle actin (α-SMA), desmin, vimentin, and caldesmon, and transmission electron microscopy (TEM). The phenotype of fibroblasts subcultured in a serum free medium from pterygium fibrovascular tissues was characterised by the above antibodies. Bundles of dense fibrous tissues were noted in 86% of the fibrovascular tissue specimens evaluated. Cells within these bundles were characterised as myofibroblasts based on positive staining to α-SMA, but negative to desmin and caldesmon, markers for smooth muscle cells. Interestingly, positive α-SMA staining was also found in the periorbital fibroadipose tissue posterior to Tenon’s capsule near the nasal conjunctiva in all exenterated specimens. All first passage fibroblasts expressed vimentin, some were positive to α-SMA, but all were negative to desmin or caldesmon. Cells in pterygium fibrovascular tissues showed ultrastructural features of intracytoplasmic bundles of microfilaments, consistent with myofibroblastic differentiation. Conclusion: These studies collectively demonstrate the presence of contractile myofibroblasts bundle in pterygia and in the periorbital fibroadipose tissue posterior to Tenon’s capsule of exenterated eyes without pterygium.
Bibliography:	istex:2B78B15ABA4D1C54D52E9901A1495E3BCCF7E6B6 href:bjophthalmol-89-269.pdf ark:/67375/NVC-5WTT70CF-V PMID:15722301 Correspondence to: Scheffer C G Tseng MD PhD Ocular Surface Center, 7000 SW 97 Avenue, Suite 213, Miami, FL 33173, USA; stseng@ocularsurface.com local:0890269 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Presented in part at the Annual Meeting of International Ocular Surface Society, May 2002 in Fort Lauderdale, FL, and at the ARVO Meeting, May 2003 in Fort Lauderdale, FL, USA. Financial support in part by an unrestricted grant from Ocular Surface Research and Education Foundation, Miami, FL, USA. Correspondence to: Scheffer C G Tseng MD PhD Ocular Surface Center, 7000 SW 97 Avenue, Suite 213, Miami, FL 33173, USA; stseng@ocularsurface.com Conflict of interest: None.
ISSN:	0007-1161 1468-2079
DOI:	10.1136/bjo.2004.050633