Search Results - "Kawashima, Shigehiro A."

Phosphorylation of H2A by Bub1 Prevents Chromosomal Instability Through Localizing Shugoshin by Kawashima, Shigehiro A, Yamagishi, Yuya, Honda, Takashi, Ishiguro, Kei-ichiro, Watanabe, Yoshinori

“…Bub1 is a multi-task protein kinase required for proper chromosome segregation in eukaryotes. Impairment of Bub1 in humans may lead to chromosomal instability…”
Get full text

Leading approaches in synthetic epigenetics for novel therapeutic strategies by Yamatsugu, Kenzo, Kawashima, Shigehiro A, Kanai, Motomu

“…In recent years, our knowledge of the epigenetic functions regulated by post-translational modifications (PTMs) of histones, and their role in various…”
Get full text

Potent, Reversible, and Specific Chemical Inhibitors of Eukaryotic Ribosome Biogenesis by Kawashima, Shigehiro A., Chen, Zhen, Aoi, Yuki, Patgiri, Anupam, Kobayashi, Yuki, Nurse, Paul, Kapoor, Tarun M.

“…All cellular proteins are synthesized by ribosomes, whose biogenesis in eukaryotes is a complex multi-step process completed within minutes. Several chemical…”
Get full text

A chemical catalyst enabling histone acylation with endogenous acyl-CoA by Habazaki, Misuzu, Mizumoto, Shinsuke, Kajino, Hidetoshi, Kujirai, Tomoya, Kurumizaka, Hitoshi, Kawashima, Shigehiro A., Yamatsugu, Kenzo, Kanai, Motomu

“…Life emerges from a network of biomolecules and chemical reactions catalyzed by enzymes. As enzyme abnormalities are often connected to various diseases, a…”
Get full text

Live-cell epigenome manipulation by synthetic histone acetylation catalyst system by Fujiwara, Yusuke, Yamanashi, Yuki, Fujimura, Akiko, Sato, Yuko, Kujirai, Tomoya, Kurumizaka, Hitoshi, Kimura, Hiroshi, Yamatsugu, Kenzo, Kawashima, Shigehiro A., Kanai, Motomu

“…Chemical modifications of histones, such as lysine acetylation and ubiquitination, play pivotal roles in epigenetic regulation of gene expression. Methods to…”
Get full text

Synthetic Posttranslational Modifications: Chemical Catalyst-Driven Regioselective Histone Acylation of Native Chromatin by Amamoto, Yoshifumi, Aoi, Yuki, Nagashima, Nozomu, Suto, Hiroki, Yoshidome, Daisuke, Arimura, Yasuhiro, Osakabe, Akihisa, Kato, Daiki, Kurumizaka, Hitoshi, Kawashima, Shigehiro A, Yamatsugu, Kenzo, Kanai, Motomu

“…Posttranslational modifications (PTMs) of histones play an important role in the complex regulatory mechanisms governing gene transcription, and their…”
Get full text

Bub1 kinase- and H2A phosphorylation-independent regulation of Shugoshin proteins under glucose-restricted conditions by Kobayashi, Yuki, Kawashima, Shigehiro A.

“…Shugoshin family proteins are involved in various aspects of chromatin regulations, such as chromosome segregation, chromatin structure, and gene expression…”
Get full text

LC–MS/MS-based quantitative study of the acyl group- and site-selectivity of human sirtuins to acylated nucleosomes by Tanabe, Kana, Liu, Jiaan, Kato, Daiki, Kurumizaka, Hitoshi, Yamatsugu, Kenzo, Kanai, Motomu, Kawashima, Shigehiro A.

“…Chromatin structure and gene expression are dynamically regulated by posttranslational modifications of histones. Recent advance in mass spectrometry has…”
Get full text

Nuclear envelope expansion is crucial for proper chromosomal segregation during a closed mitosis by Takemoto, Ai, Kawashima, Shigehiro A, Li, Juan-Juan, Jeffery, Linda, Yamatsugu, Kenzo, Elemento, Olivier, Nurse, Paul

“…Here, we screened a 10,371 library of diverse molecules using a drug-sensitive fission yeast strain to identify compounds which cause defects in chromosome…”
Get full text

Malonylation of histone H2A at lysine 119 inhibits Bub1-dependent H2A phosphorylation and chromosomal localization of shugoshin proteins by Ishiguro, Tadashi, Tanabe, Kana, Kobayashi, Yuki, Mizumoto, Shinsuke, Kanai, Motomu, Kawashima, Shigehiro A.

“…Post-translational modifications of histones, such as acetylation and phosphorylation, are highly conserved in eukaryotes and their combination enables precise…”
Get full text

The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis by Watanabe, Yoshinori, Kitajima, Tomoya S, Kawashima, Shigehiro A

“…Meiosis comprises a pair of specialized nuclear divisions that produce haploid germ cells. To accomplish this, sister chromatids must segregate together during…”
Get full text

Site-Selective Synthetic Acylation of a Target Protein in Living Cells Promoted by a Chemical Catalyst/Donor System by Hamajima, Wataru, Fujimura, Akiko, Fujiwara, Yusuke, Yamatsugu, Kenzo, Kawashima, Shigehiro A, Kanai, Motomu

“…Cell biology is tightly regulated by post-translational modifications of proteins. Methods to modulate post-translational modifications in living cells without…”
Get full text

Shugoshin enables tension-generating attachment of kinetochores by loading Aurora to centromeres by Kawashima, Shigehiro A, Tsukahara, Tatsuya, Langegger, Maria, Hauf, Silke, Kitajima, Tomoya S, Watanabe, Yoshinori

“…Fission yeast shugoshin Sgo1 is meiosis specific and cooperates with protein phosphatase 2A to protect centromeric cohesin at meiosis I. The other…”
Get full text

Hydroxamic Acid‐Piperidine Conjugate is an Activated Catalyst for Lysine Acetylation under Physiological Conditions by Mizumoto, Shinsuke, Xi, Siqi, Fujiwara, Yusuke, Kawashima, Shigehiro A., Yamatsugu, Kenzo, Kanai, Motomu

“…Lysine acylation of proteins is an essential chemical reaction for posttranslational modification and as a means of protein modification in various…”
Get full text

Leuco Ethyl Violet as Self‐Activating Prodrug Photocatalyst for In Vivo Amyloid‐Selective Oxygenation by Furuta, Masahiro, Arii, Suguru, Umeda, Hiroki, Matsukawa, Ryota, Shizu, Katsuyuki, Kaji, Hironori, Kawashima, Shigehiro A., Hori, Yukiko, Tomita, Taisuke, Sohma, Youhei, Mitsunuma, Harunobu, Kanai, Motomu

“…Aberrant aggregates of amyloid‐β (Aβ) and tau protein (tau), called amyloid, are related to the etiology of Alzheimer disease (AD). Reducing amyloid levels in…”
Get full text

Actin filaments accumulated in the nucleus remain in the vicinity of condensing chromosomes in the zebrafish early embryo by Oda, Haruka, Sato, Yuko, Kawashima, Shigehiro A, Fujiwara, Yusuke, Pálfy, Máté, Wu, Edlyn, Vastenhouw, Nadine L, Kanai, Motomu, Kimura, Hiroshi

“…In the cytoplasm, filamentous actin (F-actin) plays a critical role in cell regulation, including cell migration, stress fiber formation, and cytokinesis…”
Get full text

Supramolecular Ligands for Histone Tails by Employing a Multivalent Display of Trisulfonated Calix[4]arenes by Kimura, Yasuaki, Saito, Nae, Hanada, Kayo, Liu, Jiaan, Okabe, Takayoshi, Kawashima, Shigehiro A., Yamatsugu, Kenzo, Kanai, Motomu

“…Post‐translational modification of histone tails plays critical roles in gene regulation. Thus, molecules recognizing histone tails and controlling their…”
Get full text

Optimization of the analogue-sensitive Cdc2/Cdk1 mutant by in vivo selection eliminates physiological limitations to its use in cell cycle analysis by Aoi, Yuki, Kawashima, Shigehiro A., Simanis, Viesturs, Yamamoto, Masayuki, Sato, Masamitsu

“…Analogue-sensitive (as) mutants of kinases are widely used to selectively inhibit a single kinase with few off-target effects. The analogue-sensitive mutant…”
Get full text

Improving Drug Sensitivity of HIV-1 Protease Inhibitors by Restriction of Cellular Efflux System in a Fission Yeast Model by Zhang, Jiantao, Li, Qi, Kawashima, Shigehiro A., Nasr, Mohamed, Xue, Fengtian, Zhao, Richard Y.

“…Fission yeast can be used as a cell-based system for high-throughput drug screening. However, higher drug concentrations are often needed to achieve the same…”
Get full text

Dissecting the first and the second meiotic divisions using a marker-less drug-hypersensitive fission yeast by Aoi, Yuki, Sato, Masamitsu, Sutani, Takashi, Shirahige, Katsuhiko, Kapoor, Tarun M, Kawashima, Shigehiro A

“…Faithful chromosome segregation during meiosis is indispensable to prevent birth defects and infertility. Canonical genetic manipulations have not been very…”
Get full text