High-Affinity Peptide Ligands to Prostate-Specific Antigen Identified by Polysome Selection

We have used the polysome-selection method to isolate peptide ligands that bind with high affinity to Prostate-Specific Antigen (PSA), an important prostate-cancer marker. Two random libraries, each encoding approximately 1012random peptides, were transcribed into RNA and translatedin vitro.Polysome...

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Bibliographic Details
Published in:	Biochemical and biophysical research communications Vol. 232; no. 2; pp. 578 - 582
Main Authors:	Gersuk, Geoffrey M., Corey, Michael J., Corey, Eva, Stray, James E., Kawasaki, Glenn H., Vessella, Robert L.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 17-03-1997
Subjects:	Amino Acid Sequence Binding, Competitive Cloning, Molecular Ligands Molecular Sequence Data Peptides - chemical synthesis Peptides - genetics Peptides - metabolism Polyribosomes - metabolism Prostate-Specific Antigen - metabolism Protein Binding
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Summary:	We have used the polysome-selection method to isolate peptide ligands that bind with high affinity to Prostate-Specific Antigen (PSA), an important prostate-cancer marker. Two random libraries, each encoding approximately 1012random peptides, were transcribed into RNA and translatedin vitro.Polysomes were panned by affinity selection of the nascent peptides against immobilized PSA. Over 30% of the selected species had significant affinity for PSA; the dissociation constant of the complex formed by the best isolate with PSA was <10−9M. Formation of streptavidin conjugates of selected peptides improved their affinities and, in one case, virtually eliminated non-specific binding. The polysome-selection method can be used to produce high-affinity peptide ligands of potential use in diagnostic and therapeutic procedures.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0006-291X 1090-2104
DOI:	10.1006/bbrc.1997.6331