Role of berberine nanoformulation in epilepsy: A novel therapeutic strategy

The aim of the present study was to develop a novel formulation of berberine (BBR) and demonstrate its anti-seizure effect in pentylenetetrazole (PTZ) induced kindling model in rats. Nanoparticles of BBR were formulated using Poly Lactic-co-Glycolic Acid (PLGA) as a polymer. Emulsification and solve...

Full description

Saved in:

Bibliographic Details
Published in:	Epilepsy research Vol. 205; p. 107419
Main Authors:	Saha, Lekha, kumari, Puja, Sinha, V.R., Gautam, Vipasha, Kaur, Lavjot, Sharma, Sunil, Chakrabarti, Amitava
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-09-2024
Subjects:	Berberine Berberine nanoparticles Bioavailability Epileptogenesis PLGA PTZ kindling Berberine Epileptogenesis Berberine nanoparticles PLGA Bioavailability PTZ kindling
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The aim of the present study was to develop a novel formulation of berberine (BBR) and demonstrate its anti-seizure effect in pentylenetetrazole (PTZ) induced kindling model in rats. Nanoparticles of BBR were formulated using Poly Lactic-co-Glycolic Acid (PLGA) as a polymer. Emulsification and solvent evaporation technique was used. PTZ induced kindling model in male wistar rat was used to demonstrate the anti-seizure effect of nano-BBR. The particle size obtained for the final formulation was 242.8 ± 67.35 nm with a PDI of 0.140 ± 0.01. PLGA encapsulated BBR nanoparticles showed the % encapsulation efficiency of 87.33 ± 2.42 % and % drug loading of 48.47 ± 1.34 %. In-vitro drug release data showed sustained release of nano-BBR as compared to BBR. Kinetic study data showed increase in AUC of nano-BBR (35,429.46 h.ng/ml) as compared to BBR (28,211.07 h.ng/ml). Cmax for nano- BBR (2251.90 ng/ml) is approximately 1.6 times greater than BBR (1505.50 ng/ml). Nano- BBR has shown the significant effect on the seizure score. The PLGA encapsulated berberine nanoparticles were prepared by an innovative simple method and offers excellent potential as an antiepileptic agent. [Display omitted] •In the present study nanoformulation of BBR (Nano-BBR) developed•Nano-BBR showed better PK parameters and increased bioavailability as compared to BBR.•Nano-BBR lowers the seizure severity in PTZ kindling model in rats.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0920-1211 1872-6844 1872-6844
DOI:	10.1016/j.eplepsyres.2024.107419