A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis

Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study hi...

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Bibliographic Details

Published in:	Brazilian journal of microbiology Vol. 41; no. 2; pp. 295 - 299
Main Authors:	Kumar, Gavish(National JALMA Institute for Leprosy and Other Mycobacterial Diseases Department of Immunology), Shankar, Hari(National JALMA Institute for Leprosy and Other Mycobacterial Diseases Department of Immunology), Bisht, Deepa(National JALMA Institute for Leprosy and Other Mycobacterial Diseases Department of Biochemistry), Sharma, Prashant(National JALMA Institute for Leprosy and Other Mycobacterial Diseases Department of Biochemistry), Singhal, Neelja(National JALMA Institute for Leprosy and Other Mycobacterial Diseases Department of Biochemistry), Katoch, V.M.(Indian Council of Medical Research Department of Health Research), Joshi, Beenu(National JALMA Institute for Leprosy and Other Mycobacterial Diseases Department of Immunology)
Format:	Journal Article
Language:	English
Published:	Brazil Sociedade Brasileira de Microbiologia 01-04-2010 Springer Nature B.V
Subjects:	Bacteria Culture filtrate proteins Lipids M. tuberculosis Medical Microbiology MICROBIOLOGY Proteins SDS-TCA procedure Short Communication Tuberculosis Two-dimensional gel electrophoresis Culture filtrate proteins SDS-TCA procedure M. tuberculosis Two-dimensional gel electrophoresis
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