Search Results - "Kast, David"

The Cytoskeleton–Autophagy Connection by Kast, David J., Dominguez, Roberto

“…Actin cytoskeleton dynamics play vital roles in most forms of intracellular trafficking by promoting the biogenesis and transport of vesicular cargoes…”
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Mechanism of IRSp53 inhibition by 14-3-3 by Kast, David J., Dominguez, Roberto

“…Filopodia are precursors of dendritic spines and polarized cell migration. The I-BAR-domain protein IRSp53 is a key regulator of filopodia dynamics that…”
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Purification of modified mammalian actin isoforms for in vitro reconstitution assays by Kast, David J., Jansen, Silvia

“…In vitro reconstitution assays using purified actin have greatly improved our understanding of cytoskeletal dynamics and their regulation by actin-binding…”
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IRSp53 coordinates AMPK and 14-3-3 signaling to regulate filopodia dynamics and directed cell migration by Kast, David J, Dominguez, Roberto

“…Filopodia are actin-filled membrane protrusions that play essential roles in cell motility and cell-cell communication and act as precursors of dendritic…”
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WHAMM Directs the Arp2/3 Complex to the ER for Autophagosome Biogenesis through an Actin Comet Tail Mechanism by Kast, David J., Zajac, Allison L., Holzbaur, Erika L.F., Ostap, E. Michael, Dominguez, Roberto

“…Nucleation-promoting factors (NPFs) control the spatio-temporal activity of Arp2/3 complex in cells [1, 2]. Thus, WASP and the WAVE complex direct the…”
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Structural analysis of the transitional state of Arp2/3 complex activation by two actin-bound WCAs by Boczkowska, Malgorzata, Rebowski, Grzegorz, Kast, David J., Dominguez, Roberto

“…Actin filament nucleation and branching by Arp2/3 complex is activated by nucleation-promoting factors (NPFs), whose C-terminal WCA region contains binding…”
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IST1 regulates select recycling pathways by Clippinger, Amy K., Naismith, Teresa V., Yoo, Wonjin, Jansen, Silvia, Kast, David J., Hanson, Phyllis I.

“…ESCRTs (Endosomal Sorting Complex Required for Transports) are a modular set of protein complexes with membrane remodeling activities that include the…”
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WHAMM links actin assembly via the Arp2/3 complex to autophagy by Kast, David J, Dominguez, Roberto

“…Macroautophagy (hereafter autophagy) is the process by which cytosolic material destined for degradation is enclosed inside a double-membrane cisterna known as…”
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Mechanism of IRSp53 inhibition and combinatorial activation by Cdc42 and downstream effectors by Kast, David J, Yang, Changsong, Disanza, Andrea, Boczkowska, Malgorzata, Madasu, Yadaiah, Scita, Giorgio, Svitkina, Tatyana, Dominguez, Roberto

“…IRSp53 is a BAR-domain protein under the control of Rho family GTPases and has crucial roles in processes such as cell motility and tumor invasiveness. In a…”
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Rickettsia Sca2 has evolved formin-like activity through a different molecular mechanism by Madasu, Yadaiah, Suarez, Cristian, Kast, David J, Kovar, David R, Dominguez, Roberto

“…Sca2 (surface cell antigen 2) is the only bacterial protein known to promote both actin filament nucleation and profilin-dependent elongation, mimicking…”
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Subunit Rtt102 Controls the Conformation of the Arp7/9 Heterodimer and Its Interactions with Nucleotide and the Catalytic Subunit of SWI/SNF Remodelers by Turegun, Bengi, Kast, David J., Dominguez, Roberto

“…Chromatin-remodeling complexes are assembled around a catalytic subunit that contains a central ATPase domain and flanking sequences that recruit auxiliary…”
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CDC42 switches IRSp53 from inhibition of actin growth to elongation by clustering of VASP by Disanza, Andrea, Bisi, Sara, Winterhoff, Moritz, Milanesi, Francesca, Ushakov, Dmitry S, Kast, David, Marighetti, Paola, Romet-Lemonne, Guillaume, Müller, Hans-Michael, Nickel, Walter, Linkner, Joern, Waterschoot, Davy, Ampè, Christophe, Cortellino, Salvatore, Palamidessi, Andrea, Dominguez, Roberto, Carlier, Marie-France, Faix, Jan, Scita, Giorgio

“…Filopodia explore the environment, sensing soluble and mechanical cues during directional motility and tissue morphogenesis. How filopodia are initiated and…”
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Phosphorylation-induced structural changes in smooth muscle myosin regulatory light chain by Kast, David, Espinoza-Fonseca, L. Michel, Yi, Christina, Thomas, David D

“…We have performed complementary time-resolved fluorescence resonance energy transfer (TR-FRET) experiments and molecular dynamics (MD) simulations to elucidate…”
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Arp you ready for actin in the nucleus? by Kast, David J, Dominguez, Roberto

“…EMBO J 30 11, 2153-2166 (2011); published online 15 April 2011Actin and actin-related proteins (Arps) are critical components of chromatin remodelling and…”
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High-performance time-resolved fluorescence by direct waveform recording by Muretta, Joseph M, Kyrychenko, Alexander, Ladokhin, Alexey S, Kast, David J, Gillispie, Gregory D, Thomas, David D

“…We describe a high-performance time-resolved fluorescence (HPTRF) spectrometer that dramatically increases the rate at which precise and accurate…”
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Thermodynamic and Structural Basis of Phosphorylation-Induced Disorder-to-Order Transition in the Regulatory Light Chain of Smooth Muscle Myosin by Espinoza-Fonseca, L. Michel, Kast, David, Thomas, David D

“…We have performed molecular dynamics simulations of the phosphorylation domain (PD) of the regulatory light chain (RLC) of smooth muscle myosin, to gain…”
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Site-directed spectroscopic probes of actomyosin structural dynamics by Thomas, David D, Kast, David, Korman, Vicci L

“…Spectroscopy of myosin and actin has entered a golden age. High-resolution crystal structures of isolated actin and myosin have been used to construct detailed…”
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Molecular Dynamics Simulations Reveal a Disorder-to-Order Transition on Phosphorylation of Smooth Muscle Myosin by Michel Espinoza-Fonseca, L., Kast, David, Thomas, David D.

“…We have performed molecular dynamics simulations of the phosphorylated (at S-19) and the unphosphorylated 25-residue N-terminal phosphorylation domain of the…”
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Interactions of Nuclear Actin-Related Proteins with SWI/SNF Chromatin-Remodeling Complexes by Turegun, Bengi, Kast, David J., Dominguez, Roberto

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Rickettsia Sca2 has evolved formin-like activity through a different molecular mechanism by Madasu, Yadaiah, Suarez, Cristian, Kast, David J., Kovar, David R., Dominguez, Roberto

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