Effect of magnetic field on the growth of the cultured Entamoeba histolytica isolated from patients in Palestine

Static magnetic field (SMF) is generated in vicinity of moving charge or current passing through conductor. In this study, we aimed to investigate the effect of SMF on the growth of the cultured Entamoeba histolytica (E. histolytica) trophozoites. Different SMF strengths with maximum value equals 30...

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Bibliographic Details
Published in:	Experimental parasitology Vol. 226-227; p. 108126
Main Authors:	Al-Hindi, Adnan I., El-Khozondar, Hala J., Tabaza, Wael, Alreefi, Mariam, Al Afifi, Ahmed M., Kassem, Doha, Al-Bahri, Reham, Abu Haseera, Ghadeer
Format:	Journal Article
Language:	English
Published:	Elsevier Inc 01-07-2021
Subjects:	Antiamoebic activity Bowel lumen Bowel wall Entamoeba histolytica Static magnetic field Tissues Entamoeba histolytica Bowel wall Static magnetic field Tissues Bowel lumen Antiamoebic activity
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Summary:	Static magnetic field (SMF) is generated in vicinity of moving charge or current passing through conductor. In this study, we aimed to investigate the effect of SMF on the growth of the cultured Entamoeba histolytica (E. histolytica) trophozoites. Different SMF strengths with maximum value equals 30 mT (mT) was applied on the E.histolytica for different periods of times: 0 h, 24 h, 48 h, and 72 h. A modified diphasic liver infusion agar medium was used for culturing E. histolytica in vitro. The results showed the successful stabilization of culture of E. histolytica trophozoites. If we kept the sample for longer time, e. g. 14 days, the growth rate decreases to zero. When applying 10 mT and 15 mT SMF on the sample, it is found that the cultivated E. histolytica trophozoites dies after 4 and 2 days respectively. The experiments suggested that the SMF inhibited the growth and the propagation of E. histolytica cells. In addition, it completely killed all the cells in a short time interval which depend on the SMF strength. It is concluded that the SMFs inhibits the growth of E. histolytica and change the morphology of these cells. Thus, we recommend to use SMF as treatment to mitigate the growth of E. histolytica. [Display omitted] A modified diphasic liver infusion agar medium was used for culturing E. histolytica in vitro.Different SMF strengths with maximum value equals 30 mT (mT) was applied on the E-histolytica for different periods of times: zero hours (hrs), 24 h, 48 h, and 72 h.Without SMF, E-histolytica dies after 14 days.Applying 10 mT and 15 mT cause death of the E-histolytica after 4 and 2 days respectivelyThe experiments suggested that the SMF inhibited the growth and the propagation of E. histolytica cells and completely killed all the cells in a short time interval which depend on the SMF strength.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-4894 1090-2449
DOI:	10.1016/j.exppara.2021.108126