Search Results - "Kashina, Anna S"

Dynamics of intracellular stress-induced tRNA trafficking by Dhakal, Rabin, Tong, Chunyi, Anderson, Sean, Kashina, Anna S, Cooperman, Barry, Bau, Haim H

“…Abstract Stress is known to induce retrograde tRNA translocation from the cytoplasm to the nucleus but translocation kinetics and tRNA-spatial distribution…”
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Regulation of actin isoforms in cellular and developmental processes by Kashina, Anna S.

“…Actin is one of the most abundant and essential intracellular proteins that mediates nearly every form of cellular movement and underlies such key processes as…”
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Differential N-terminal processing of beta and gamma actin by Chen, Li, Vedula, Pavan, Tang, Hsin Yao, Dong, Dawei W., Kashina, Anna S.

“…Cytoplasmic beta- and gamma-actin are ubiquitously expressed in every eukaryotic cell. They are encoded by different genes, but their amino acid sequences…”
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Assaying ATE1 Activity in Yeast by β-Gal Degradation by Kashina, Anna S

“…In the 1980s, it was found that addition of N-terminal Arg to proteins induces their ubiquitination and degradation by the N-end rule pathway. While this…”
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Protein Arginylation: Milestones of Discovery by Kashina, Anna S

“…Posttranslational modifications have emerged in recent years as the major biological regulators responsible for the orders of magnitude increase in complexity…”
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Preparation of ATE1 Enzyme from Native Mammalian Tissues by Kashina, Anna S

“…Early studies of protein arginylation preceded the wide availability of recombinant protein expression and relied heavily on the fractionation of proteins from…”
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Development of New Tools for the Studies of Protein Arginylation by Kashina, Anna S

“…Studies of posttranslational modifications present many unique challenges, stemming from their role as the major drivers of biological complexity. Perhaps the…”
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An Essential Role of N-Terminal Arginylation in Cardiovascular Development by Kwon, Yong Tae, Kashina, Anna S., Davydov, Ilia V., Hu, Rong-Gui, An, Jee Young, Seo, Jai Wha, Du, Fangyong, Varshavsky, Alexander

“…The enzymatic conjugation of arginine to the N-termini of proteins is a part of the ubiquitin-dependent N-end rule pathway of protein degradation. In mammals,…”
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High-Throughput Arginylation Assay in Microplate Format by Saha, Sougata, Wang, Junling, Kashina, Anna S

“…Here, we describe the biochemical assay for ATE1-mediated arginylation in microplate format, which can be applied to high-throughput screens for the…”
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Assaying Intracellular Arginylation Activity Using a Fluorescent Reporter by MacTaggart, Brittany, Kashina, Anna S

“…In this chapter, we present a simplified version of the method described in Chapter 9 of this book, adapted for fast and convenient evaluation of intracellular…”
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Assaying ATE1 Activity In Vitro by Wang, Junling, Kashina, Anna S

“…Here, we describe a standard arginyltransferase assay in vitro using bacterially expressed purified ATE1 in a system with a minimal number of components (Arg,…”
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Bacterial Expression and Purification of Recombinant Arginyltransferase (ATE1) and Arg-tRNA Synthetase (RRS) for Arginylation Assays by Wang, Junling, Kashina, Anna S

“…Here, we describe the procedure for the expression and purification of recombinant ATE1 from E. coli. This method is easy and convenient and can result in…”
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Assaying for Arginyltransferase Activity and Specificity by Peptide Arrays by Wang, Junling, Kashina, Anna S

“…Here, we describe arginylation assays performed on peptide arrays immobilized on cellulose membranes via chemical synthesis. In this assay, it is possible to…”
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N-terminal acetylation and arginylation of actin determines the architecture and assembly rate of linear and branched actin networks by Chin, Samantha M., Hatano, Tomoyuki, Sivashanmugam, Lavanya, Suchenko, Andrejus, Kashina, Anna S., Balasubramanian, Mohan K., Jansen, Silvia

“…The great diversity in actin network architectures and dynamics is exploited by cells to drive fundamental biological processes, including cell migration,…”
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Identification of Arginylated Proteins by Mass Spectrometry by Kashina, Anna S, Yates Iii, John R

“…Here, we describe the method for the identification of arginylated proteins by mass spectrometry. This method has been originally applied to the identification…”
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Protein Arginylation: Over 50 Years of Discovery by Kashina, Anna S

“…Posttranslational modifications have emerged in recent years as the major biological regulators responsible for the orders of magnitude increase in complexity…”
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Analysis of Arginylated Peptides by Subtractive Edman Degradation by Kashina, Anna S, Yates Iii, John R

“…During the early studies of N-terminal arginylation, Edman degradation was widely used to identify N-terminally added Arg on protein substrates. This old…”
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Development of New Tools for the Studies of Protein Arginylation by Kashina, Anna S

“…Studies of posttranslational modifications present many unique challenges, stemming from their role as the major drivers of biological complexity. Perhaps the…”
Get more information

Assaying ATE1 Activity in Yeast by β-Gal Degradation by Kashina, Anna S

“…In 1980s it was found that addition of N-terminal Arg to proteins induces their ubiquitination and degradation by the N-end rule pathway. While this mechanism…”
Get more information

Preparation of ATE1 Enzyme from Native Mammalian Tissues by Kashina, Anna S

“…Early studies of protein arginylation preceded the wide use of recombinant protein expression and relied heavily on fractionation of proteins from native…”
Get more information