Search Results - "Kasai, Rinshi S."

Super-long single-molecule tracking reveals dynamic-anchorage-induced integrin function by Tsunoyama, Taka A., Watanabe, Yusuke, Goto, Junri, Naito, Kazuma, Kasai, Rinshi S., Suzuki, Kenichi G. N., Fujiwara, Takahiro K., Kusumi, Akihiro

“…Single-fluorescent-molecule imaging tracking (SMT) is becoming an important tool to study living cells. However, photobleaching and photoblinking (hereafter…”
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High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases by Koyama-Honda, Ikuko, Fujiwara, Takahiro K, Kasai, Rinshi S, Suzuki, Kenichi G N, Kajikawa, Eriko, Tsuboi, Hisae, Tsunoyama, Taka A, Kusumi, Akihiro

“…Using single-molecule imaging with enhanced time resolutions down to 5 ms, we found that CD59 cluster rafts and GM1 cluster rafts were stably induced in the…”
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Defining raft domains in the plasma membrane by Kusumi, Akihiro, Fujiwara, Takahiro K., Tsunoyama, Taka A., Kasai, Rinshi S., Liu, An‐An, Hirosawa, Koichiro M., Kinoshita, Masanao, Matsumori, Nobuaki, Komura, Naoko, Ando, Hiromune, Suzuki, Kenichi G. N.

“…Many plasma membrane (PM) functions depend on the cholesterol concentration in the PM in strikingly nonlinear, cooperative ways: fully functional in the…”
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Hierarchical mesoscale domain organization of the plasma membrane by Kusumi, Akihiro, Suzuki, Kenichi G.N., Kasai, Rinshi S., Ritchie, Ken, Fujiwara, Takahiro K.

“…Based on recent single-molecule imaging results in the living cell plasma membrane, we propose a hierarchical architecture of three-tiered mesoscale (2–300 nm)…”
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The Class-A GPCR Dopamine D2 Receptor Forms Transient Dimers Stabilized by Agonists: Detection by Single-Molecule Tracking by Kasai, Rinshi S., Ito, Shuichi V., Awane, Ryo M., Fujiwara, Takahiro K., Kusumi, Akihiro

“…Whether class-A G-protein coupled receptors (GPCRs) exist and work as monomers or dimers has drawn extensive attention. A class-A GPCR dopamine D2 receptor…”
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Full characterization of GPCR monomer-dimer dynamic equilibrium by single molecule imaging by Kasai, Rinshi S, Suzuki, Kenichi G.N, Prossnitz, Eric R, Koyama-Honda, Ikuko, Nakada, Chieko, Fujiwara, Takahiro K, Kusumi, Akihiro

“…Receptor dimerization is important for many signaling pathways. However, the monomer-dimer equilibrium has never been fully characterized for any receptor with…”
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Transient GPI-anchored protein homodimers are units for raft organization and function by Suzuki, Kenichi G N, Kasai, Rinshi S, Hirosawa, Koichiro M, Nemoto, Yuri L, Ishibashi, Munenori, Miwa, Yoshihiro, Fujiwara, Takahiro K, Kusumi, Akihiro

“…Single-molecule imaging reveals that GPI-anchored proteins in the plasma membrane form homodimer rafts via ectodomain protein interactions. Raft-lipid…”
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ABCA1 dimer–monomer interconversion during HDL generation revealed by single-molecule imaging by Nagata, Koh O., Nakada, Chieko, Kasai, Rinshi S., Kusumi, Akihiro, Ueda, Kazumitsu

“…The generation of high-density lipoprotein (HDL), one of the most critical events for preventing atherosclerosis, is mediated by ATP-binding cassette protein…”
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A synthetic ion channel with anisotropic ligand response by Muraoka, Takahiro, Noguchi, Daiki, Kasai, Rinshi S., Sato, Kohei, Sasaki, Ryo, Tabata, Kazuhito V., Ekimoto, Toru, Ikeguchi, Mitsunori, Kamagata, Kiyoto, Hoshino, Norihisa, Noji, Hiroyuki, Akutagawa, Tomoyuki, Ichimura, Kazuaki, Kinbara, Kazushi

“…Biological membranes play pivotal roles in the cellular activities. Transmembrane proteins are the central molecules that conduct membrane-mediated biochemical…”
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A methodology for creating mutants of G‐protein coupled receptors stabilized in active state by combining statistical thermodynamics and evolutionary molecular engineering by Yamamoto, Taisei, Yasuda, Satoshi, Kasai, Rinshi S., Nakano, Ryosuke, Hikiri, Simon, Sugaya, Kanna, Hayashi, Tomohiko, Ogasawara, Satoshi, Shiroishi, Mitsunori, Fujiwara, Takahiro K., Kinoshita, Masahiro, Murata, Takeshi

“…We challenged the stabilization of a G‐protein coupled receptor (GPCR) in the active state solely by multiple amino‐acid mutations without the agonist binding…”
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Raf Inhibitors Target Ras Spatiotemporal Dynamics by Cho, Kwang-jin, Kasai, Rinshi S., Park, Jin-Hee, Chigurupati, Sravanthi, Heidorn, Sonja J., van der Hoeven, Dharini, Plowman, Sarah J., Kusumi, Akihiro, Marais, Richard, Hancock, John F.

“…The lateral segregation of Ras proteins into transient plasma membrane nanoclusters is essential for high-fidelity signal transmission by the Ras…”
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Membrane mechanisms for signal transduction: The coupling of the meso-scale raft domains to membrane-skeleton-induced compartments and dynamic protein complexes by Kusumi, Akihiro, Fujiwara, Takahiro K., Morone, Nobuhiro, Yoshida, Kenta J., Chadda, Rahul, Xie, Min, Kasai, Rinshi S., Suzuki, Kenichi G.N.

“…► The plasma membrane exhibits the three-tiered meso-scale domain architecture. ► The first tier is actin membrane-skeleton-induced compartments…”
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Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography by Morone, Nobuhiro, Fujiwara, Takahiro, Murase, Kotono, Kasai, Rinshi S, Ike, Hiroshi, Yuasa, Shigeki, Usukura, Jiro, Kusumi, Akihiro

“…Three-dimensional images of the undercoat structure on the cytoplasmic surface of the upper cell membrane of normal rat kidney fibroblast (NRK) cells and fetal…”
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Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging by Nishimura, Hirohito, Ritchie, Ken, Kasai, Rinshi S, Goto, Miki, Morone, Nobuhiro, Sugimura, Hiroyuki, Tanaka, Koichiro, Sase, Ichiro, Yoshimura, Akihiko, Nakano, Yoshitaro, Fujiwara, Takahiro K, Kusumi, Akihiro

“…Fluorescence microscopy is used extensively in cell-biological and biomedical research, but it is often plagued by three major problems with the presently…”
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Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes by Hiramoto‐Yamaki, Nao, Tanaka, Kenji A. K., Suzuki, Kenichi G. N., Hirosawa, Koichiro M., Miyahara, Manami S. H., Kalay, Ziya, Tanaka, Koichiro, Kasai, Rinshi S., Kusumi, Akihiro, Fujiwara, Takahiro K.

“…Cholesterol distribution and dynamics in the plasma membrane (PM) are poorly understood. The recent development of Bodipy488‐conjugated cholesterol molecule…”
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Accumulation of anchored proteins forms membrane diffusion barriers during neuronal polarization by Kusumi, Akihiro, Nakada, Chieko, Ritchie, Kenneth, Oba, Yuichi, Nakamura, Mitsuhiro, Hotta, Yoko, Iino, Ryota, Kasai, Rinshi S, Yamaguchi, Kazuhiko, Fujiwara, Takahiro

“…The formation and maintenance of polarized distributions of membrane proteins in the cell membrane are key to the function of polarized cells. In polarized…”
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Single-molecule imaging revealed dynamic GPCR dimerization by Kasai, Rinshi S, Kusumi, Akihiro

“…Graphical abstract…”
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Antiparallel dimer structure of CELSR cadherin in solution revealed by high-speed atomic force microscopy by Nishiguchi, Shigetaka, Kasai, Rinshi S, Uchihashi, Takayuki

“…Cadherin EGF LAG seven-pass G-type receptors (CELSR) cadherins, members of the cadherin superfamily, and adhesion G-protein-coupled receptors, play a vital…”
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Fluorescence Imaging for Monitoring the Colocalization of Two Single Molecules in Living Cells by Koyama-Honda, Ikuko, Ritchie, Ken, Fujiwara, Takahiro, Iino, Ryota, Murakoshi, Hideji, Kasai, Rinshi S., Kusumi, Akihiro

“…The interaction, binding, and colocalization of two or more molecules in living cells are essential aspects of many biological molecular processes, and…”
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Dynamic Meso-Scale Anchorage of GPI-Anchored Receptors in the Plasma Membrane: Prion Protein vs. Thy1 by Nemoto, Yuri L., Morris, Roger J., Hijikata, Hiroko, Tsunoyama, Taka A., Shibata, Akihiro C. E., Kasai, Rinshi S., Kusumi, Akihiro, Fujiwara, Takahiro K.

“…The central mechanism for the transmission of the prion protein misfolding is the structural conversion of the normal cellular prion protein to the pathogenic…”
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