Search Results - "Karunakaran, Ponniah"

Improved Broad-Host-Range RK2 Vectors Useful for High and Low Regulated Gene Expression Levels in Gram-Negative Bacteria by Blatny, Janet Martha, Brautaset, Trygve, Winther-Larsen, Hanne C., Karunakaran, Ponniah, Valla, Svein

“…This report describes the construction and use of improved broad-host-range expression vectors based on the previously constructed pJB137 and pJB653 plasmids…”
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The phenotypes of temperature-sensitive mini-RK2 replicons carrying mutations in the replication control gene trfA are suppressed nonspecifically by intragenic cop mutations by Haugan, K, Karunakaran, P, Blatny, J M, Valla, S

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Genetic antagonism and hypermutability in Mycobacterium smegmatis by Karunakaran, P, Davies, J

“…Multidrug-resistant strains of Mycobacterium tuberculosis are a serious and continuing human health problem. Such strains may contain as many as four or five…”
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Genetic Antagonism and Hypermutability inMycobacterium smegmatis by Ponniah Karunakaran, Julian Davies

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A small derivative of the broad-host-range plasmid RK2 which can be switched from a replicating to a non-replicating state as a response to an externally added inducer by Karunakaran, Ponniah, Endresen, Dag Terje, Ertesvåg, Helga, Blatny, Janet Martha, Valla, Svein

“…TrfA is the only plasmid-encoded protein required for RK2 replication. We report here the construction and characterization of an RK2-based vector in which…”
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Species-Dependent Phenotypes of Replication-Temperature-SensitivetrfA Mutants of Plasmid RK2: a Codon-Neutral Base Substitution Stimulates Temperature Sensitivity by Leading to Reduced Levels of trfA Expression by Ponniah Karunakaran, Janet Martha Blatny, Helga Ertesvåg, Svein Valla

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Species-dependent phenotypes of replication-temperature-sensitive trfA mutants of plasmid RK2: a codon-neutral base substitution stimulates temperature sensitivity by leading to reduced levels of trfA expression by Karunakaran, P, Blatny, J M, Ertesvåg, H, Valla, S

“…TrfA is the only plasmid-encoded protein required for initiation of replication of the broad-host-range plasmid RK2. Here we describe the isolation of four…”
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A small derivative of the broad-host-range plasmid RK2 which can be switched from a replicating to a non-replicating state as a response to an externally added inducer by Karunakaran, Ponniah, Endresen, Dag Terje, Ertesvåg, Helga, Blatny, Janet Martha, Valla, Svein

“…Abstract TrfA is the only plasmid-encoded protein required for RK2 replication. We report here the construction and characterization of an RK2-based vector in…”
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The Host Range of RK2 Minimal Replicon Copy-Up Mutants Is Limited by Species-Specific Differences in the Maximum Tolerable Copy Number by Haugan, Kåre, Karunakaran, Ponniah, Tøndervik, Anne, Valla, Svein

“…The minimal replicon of the broad-host-range plasmid RK2 consists of a gene, trfA ( trans-acting replication), encoding a protein required for initiation of…”
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