Search Results - "Karst, Daniel J."

Process performance and product quality in an integrated continuous antibody production process by Karst, Daniel J., Steinebach, Fabian, Soos, Miroslav, Morbidelli, Massimo

“…ABSTRACT Continuous manufacturing is currently being seriously considered in the biopharmaceutical industry as the possible new paradigm for producing…”
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Tuning metabolic efficiency for increased product yield in high titer fed‐batch Chinese hamster ovary cell culture by Helfer, Apolline, Gros, Sylvain, Kolwyck, Dave, Karst, Daniel J.

“…High demand in manufactured biologics drives the continued need for increased productivity. In this study elevated lactate metabolization resulted in improved…”
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Characterization and comparison of ATF and TFF in stirred bioreactors for continuous mammalian cell culture processes by Karst, Daniel J., Serra, Elisa, Villiger, Thomas K., Soos, Miroslav, Morbidelli, Massimo

“…[Display omitted] •Two stirred tank perfusion setups (ATF and TFF) were physically characterized.•Chosen operating parameters were confirmed by stable culture…”
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Modulation and modeling of monoclonal antibody N‐linked glycosylation in mammalian cell perfusion reactors by Karst, Daniel J., Scibona, Ernesto, Serra, Elisa, Bielser, Jean‐Marc, Souquet, Jonathan, Stettler, Matthieu, Broly, Hervé, Soos, Miroslav, Morbidelli, Massimo, Villiger, Thomas K.

“…ABSTRACT Mammalian cell perfusion cultures are gaining renewed interest as an alternative to traditional fed‐batch processes for the production of therapeutic…”
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Facile One-Step Micropatterning Using Photodegradable Gelatin Hydrogels for Improved Cardiomyocyte Organization and Alignment by Tsang, Kelly M. C., Annabi, Nasim, Ercole, Francesca, Zhou, Kun, Karst, Daniel J., Li, Fanyi, Haynes, John M., Evans, Richard A., Thissen, Helmut, Khademhosseini, Ali, Forsythe, John S.

“…Hydrogels are often employed as temporary platforms for cell proliferation and tissue organization in vitro. Researchers have incorporated photodegradable (PD)…”
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Experimental and CFD physical characterization of animal cell bioreactors: From micro- to production scale by Villiger, Thomas K., Neunstoecklin, Benjamin, Karst, Daniel J., Lucas, Eric, Stettler, Matthieu, Broly, Hervé, Morbidelli, Massimo, Soos, Miroslav

“…[Display omitted] •Characterization of bioreactors with working volumes from 15 mL up to 15′000 L.•Determination of maximum hydrodynamic stress, mixing time…”
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Transcriptome and proteome analysis of steady‐state in a perfusion CHO cell culture process by Bertrand, Vania, Karst, Daniel J., Bachmann, Alessia, Cantalupo, Katia, Soos, Miroslav, Morbidelli, Massimo

“…Long‐term continuous protein production can be reached by perfusion operation. Through the continuous removal of waste metabolites and supply of nutrients,…”
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Model based adaptive control of a continuous capture process for monoclonal antibodies production by Steinebach, Fabian, Angarita, Monica, Karst, Daniel J., Müller-Späth, Thomas, Morbidelli, Massimo

“…•It is shown how a continuous capture process can be designed and operated in a continuous integrated process with optimal performance.•A novel two column…”
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A two-step procedure for the design of perfusion bioreactors by Wolf, Moritz K.F., Pechlaner, Anna, Lorenz, Veronika, Karst, Daniel J., Souquet, Jonathan, Broly, Hervé, Morbidelli, Massimo

“…[Display omitted] •2-step procedure for the design and development of CHO cell perfusion cultures.•Step 1: Definition of suitable minimum CSPR value at…”
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Development of a shake tube‐based scale‐down model for perfusion cultures by Wolf, Moritz K.F., Lorenz, Veronika, Karst, Daniel J., Souquet, Jonathan, Broly, Hervé, Morbidelli, Massimo

“…The use of benchtop bioreactors (BRs) for the development of mammalian cell perfusion cultures is expensive and time consuming, given its complexity in…”
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Modulation of transmembrane pressure in manufacturing scale tangential flow filtration N‐1 perfusion seed culture by Karst, Daniel J., Ramer, Kevin, Hughes, Erik H., Jiang, Canping, Jacobs, Pieter J., Mitchelson, Fernie G.

“…Mammalian cells were grown to high density in a 3,000 L culture using perfusion with hollow fibers operated in a tangential flow filtration mode. The…”
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Intracellular CHO Cell Metabolite Profiling Reveals Steady‐State Dependent Metabolic Fingerprints in Perfusion Culture by Karst, Daniel J., Steinhoff, Robert F., Kopp, Marie R. G., Serra, Elisa, Soos, Miroslav, Zenobi, Renato, Morbidelli, Massimo

“…Perfusion cell culture processes allow the steady‐state culture of mammalian cells at high viable cell density, which is beneficial for overall product yields…”
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Isotope labeling to determine the dynamics of metabolic response in CHO cell perfusion bioreactors using MALDI‐TOF‐MS by Karst, Daniel J., Steinhoff, Robert F., Kopp, Marie R. G., Soos, Miroslav, Zenobi, Renato, Morbidelli, Massimo

“…The steady‐state operation of Chinese hamster ovary (CHO) cells in perfusion bioreactors requires the equilibration of reactor dynamics and cell metabolism…”
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Microarray-based MALDI-TOF mass spectrometry enables monitoring of monoclonal antibody production in batch and perfusion cell cultures by Steinhoff, Robert F., Karst, Daniel J., Steinebach, Fabian, Kopp, Marie R.G., Schmidt, Gregor W., Stettler, Alexander, Krismer, Jasmin, Soos, Miroslav, Pabst, Martin, Hierlemann, Andreas, Morbidelli, Massimo, Zenobi, Renato

“…•Microarray based MALDI-MS for metabolite and antibody monitoring shows excellent results.•Monoclonal antibody is monitored directly from cell supernatant…”
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