Search Results - "Karnoup, Anton"
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antibody produced in tobacco expressing a hybrid β-1,4-galactosyltransferase is essentially devoid of plant carbohydrate epitopes
Published in Proceedings of the National Academy of Sciences - PNAS (16-05-2006)“…N-glycosylation of a mAb may have a major impact on its therapeutic merits. Here, we demonstrate that expression of a hybrid enzyme (called xylGalT),…”
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2
Immunodepletion of high abundance proteins coupled on-line with reversed-phase liquid chromatography: A two-dimensional LC sample enrichment and fractionation technique for mammalian proteomics
Published in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2009)“…Proteomic analysis can be hampered by the large concentration distribution of proteins. Immunoaffinity techniques have been applied to selectively remove high…”
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3
α-Helix Peptide Folding and Unfolding Activation Barriers: A Nanosecond UV Resonance Raman Study
Published in Journal of the American Chemical Society (08-09-1999)“…We used UV resonance Raman spectroscopy to characterize the equilibrium conformation and the kinetics of thermal denaturation of a 21 amino acid, mainly…”
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4
A novel HPLC–UV–MS method for quantitative analysis of protein glycosylation
Published in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (15-11-2007)“…Monoclonal antibody samples derived from transgenic plants (plantibodies) may often contain significant amounts of aglycosylated variants. Because glycosylated…”
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5
O-Linked glycosylation in maize-expressed human IgA1
Published in Glycobiology (Oxford) (01-10-2005)“…O-Linked glycans vary between eukaryotic cell types and play an important role in determining a glycoprotein's properties, including stability, target…”
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6
Sequence microheterogeneity of parvalbumin pI 5.0 of pike: A mass spectrometric study
Published in Biochimica et biophysica acta (2009)“…Parvalbumin (PA) is a muscle and neuronal calcium-binding protein, the major fish and frog allergen. Its characteristic feature is the presence of multiple…”
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7
Anion-induced folding of Staphylococcal nuclease: characterization of multiple equilibrium partially folded intermediates
Published in Journal of molecular biology (15-05-1998)“…The refolding of acid-unfolded staphylococcal nuclease (SNase) induced by anions was characterized, and revealed the existence of three different partially…”
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8
Transient UV Raman Spectroscopy Finds No Crossing Barrier between the Peptide α-Helix and Fully Random Coil Conformation
Published in Journal of the American Chemical Society (14-03-2001)“…Transient UV resonance Raman measurements excited within the amide π → π* transitions of a 21 unit α-helical peptide has for the first time determined a lower…”
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9
Nanosecond UV Resonance Raman Examination of Initial Steps in α-Helix Secondary Structure Evolution
Published in Journal of the American Chemical Society (28-04-1999)Get full text
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10
2DLC-UV/MS Assay for the Simultaneous Quantification of Intact Soybean Allergens Gly m 4 and Hydrophobic Protein from Soybean (HPS)
Published in Journal of agricultural and food chemistry (28-05-2014)“…Top-down approaches for quantification of proteins based on separation and mass spectrometric assays hold promise due to their high specificity and avoidance…”
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11
Quantification of Gly m 4 Protein, A Major Soybean Allergen, By Two-Dimensional Liquid Chromatography with Ultraviolet and Mass Spectrometry Detection
Published in Analytical chemistry (Washington) (20-11-2012)“…Soybean (Glycine max) is considered a major allergenic food. Gly m 4 is one of several soybean allergens that has been identified to cause an allergic…”
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12
Characterization of Aryloxyalkanoate Dioxygenase-12, a Nonheme Fe(II)/α-Ketoglutarate-Dependent Dioxygenase, Expressed in Transgenic Soybean and Pseudomonas fluorescens
Published in Journal of agricultural and food chemistry (10-07-2013)“…Aryloxyalkanoate dioxygenase-12 (AAD-12) was discovered from the soil bacterium Delftia acidovorans MC1 and is a nonheme Fe(II)/α-ketoglutarate-dependent…”
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13
Dihedral ψ Angle Dependence of the Amide III Vibration: A Uniquely Sensitive UV Resonance Raman Secondary Structural Probe
Published in Journal of the American Chemical Society (28-11-2001)“…UV resonance Raman studies of peptide and protein secondary structure demonstrate an extraordinary sensitivity of the amide III (Am III) vibration and the CαH…”
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14
An antibody produced in tobacco expressing a hybrid (beta)-1,4-galactosyltransferase is essentially devoid of plant carbohydrate epitopes
Published in Proceedings of the National Academy of Sciences - PNAS (16-05-2006)“…N-glycosylation of a mAb may have a major impact on its therapeutic merits. Here, we demonstrate that expression of a hybrid enzyme (called xylGalT),…”
Get full text
Journal Article -
15
Association of partially-folded intermediates of staphylococcal nuclease induces structure and stability
Published in Protein science (01-01-1999)“…Staphylococcal nuclease forms three different partially-folded intermediates at low pH in the presence of low to moderate concentration of anions, differing in…”
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16
Sequential Compaction of a Random Copolymer of Hydrophilic and Hydrophobic Amino Acid Residues
Published in Macromolecules (01-12-1997)“…The behavior of a high molecular weight (48.8 × 103) synthetic random copolymer composed of Glu, Leu, and Trp residues in molar ratio 82.5:16:1.5 under…”
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17
Size-exclusion chromatography of protected synthetic polypeptide tandems in an organic solvent
Published in The journal of peptide research (01-03-1997)“…A novel method for analytical and preparative size exclusion chromatography of large water‐insoluble protected peptides in an organic solvent was developed…”
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