Search Results - "Karmali, Amin"
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A Sensitive Microplate Assay for Lipase Activity Measurement Using Olive Oil Emulsion Substrate: Modification of the Copper Soap Colorimetric Method
Published in Journal of Oleo Science (2016)“…The present work involves a sensitive high-throughput microtiter plate based colorimetric assay for estimating lipase activity using cupric acetate pyridine…”
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Supercritical CO2 Extracts and Volatile Oil of Basil (Ocimum basilicum L.) Comparison with Conventional Methods
Published in Separations (01-06-2018)“…Interest in new products from aromatic plants as medical and nutritional compounds is increasing. The aim of this work was to apply different extraction…”
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Structure of Amidase from Pseudomonas aeruginosa Showing a Trapped Acyl Transfer Reaction Intermediate State
Published in The Journal of biological chemistry (06-07-2007)“…Microbial amidases belong to the thiol nitrilases family and have potential biotechnological applications in chemical and pharmaceutical industries as well as…”
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Monoclonal antibodies recognize conformational epitopes on wild-type and recombinant mutant amidases from Pseudomonas aeruginosa
Published in Molecular biotechnology (01-10-2007)“…Hybridoma technology was used to raise monoclonal antibodies (MAbs) against wild-type amidase from Pseudomonas aeruginosa. Hybridoma clones secreting…”
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Optimisation and economic assessment of lipase-catalysed production of monoesters using Rhizomucor miehei lipase in a solvent-free system
Published in Journal of cleaner production (20-11-2016)“…Enzymatic production of glycerin monolaurate (GML) was carried out by lipase catalysed esterification of lauric acid and glycerin in a solvent-free media…”
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A high throughput colorimetric assay of β-1,3-d-glucans by Congo red dye
Published in Journal of microbiological methods (01-02-2015)“…Mushroom strains contain complex nutritional biomolecules with a wide spectrum of therapeutic and prophylactic properties. Among these compounds, β-d-glucans…”
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Non-enzymatic assay for glucose by using immobilized whole-cells of E. coli containing glucose binding protein fused to fluorescent proteins
Published in Sensors and actuators. B, Chemical (31-12-2015)“…•Immobilization of whole-cells and cell-free extract containing FRET nanosensors.•Genetically encoded nanosensors immobilized on onion membrane and 96-well…”
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Purification and Partial Characterization of Peroxidases from Three Food Waste By-Products: Broad Bean Pods, Pea Pods, and Artichoke Stems
Published in Applied biochemistry and biotechnology (01-10-2019)“…In this study, peroxidases (PODs) from three waste by-products: broad bean pods (BBP), pea pods (PP), and artichoke stems (ARS) were purified and their optimal…”
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Production, purification and characterisation of polysaccharides from Pleurotus ostreatus with antitumour activity
Published in Journal of the science of food and agriculture (01-07-2012)“…BACKGROUND: Mushroom polysaccharides play an important role in functional foods because they exhibit biological modulator properties such as antitumour,…”
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A novel colorimetric assay of [beta]-D-glucans in basidiomycete strains by alcian blue dye in a 96-well microtiter plate
Published in Biotechnology progress (01-11-2015)“…Basidiomycete strains synthesize several types of [beta]-d-glucans, which play a major role in the medicinal properties of mushrooms. Therefore, the specific…”
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Investigation of structural effects and behaviour of Pseudomonas aeruginosa amidase encapsulated in reversed micelles
Published in Process biochemistry (1991) (01-02-2012)“…► Whole cells, cell free extract and purified amidase from Pseudomonas aeruginosa strains L10 and AI3 as biocatalysts for acetohydroxamic acid synthesis. ►…”
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The extracts of Gentiana lutea with potential cytotoxic effects on human carcinoma cell lines: A preliminary study
Published in European journal of integrative medicine (01-04-2019)“…Cancer is a disease with increasing incidence and is the second cause of death worldwide. Phytotherapy is based on the use of plants to treat diseases and is…”
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Novel polyol-responsive monoclonal antibodies against extracellular [beta]-d-glucans from Pleurotus ostreatus
Published in Biotechnology progress (01-01-2016)“…[beta]-d-glucans from mushroom strains play a major role as biological response modifiers in several clinical disorders. Therefore, a specific assay method is…”
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A novel colorimetric assay of β-D-glucans in basidiomycete strains by alcian blue dye in a 96-well microtiter plate
Published in Biotechnology progress (01-11-2015)“…Basidiomycete strains synthesize several types of β‐d‐glucans, which play a major role in the medicinal properties of mushrooms. Therefore, the specific…”
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Bioconversion of d-glucose into d-glucosone by Glucose 2-oxidase from Coriolus versicolor at Moderate Pressures
Published in Applied biochemistry and biotechnology (01-04-2011)“…Glucose 2-oxidase (pyranose oxidase, pyranose:oxygen-2-oxidoreductase, EC 1.1.3.10) from Coriolus versicolor catalyses the oxidation of d -glucose at carbon 2…”
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Bioconversion of d-glucose into d-glucosone by immobilized glucose 2-oxidase from Coriolus versicolor at moderate pressures
Published in Process biochemistry (1991) (2011)“…The immobilized glucose 2-oxidase (pyranose oxidase, pyranose:oxygen-2-oxidoreductase, EC 1.1.3.10) from Coriolus versicolor was used to convert d-glucose into…”
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Generation of high-affinity monoclonal antibodies of IgG class against native β-d-glucans from basidiomycete mushrooms
Published in Process biochemistry (1991) (01-02-2016)“…[Display omitted] •Novel monoclonal antibodies (Mabs) were raised against β-glucans from Pleurotus ostreatus.•Novel polyol-responsive Mabs were raised against…”
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Novel polyol-responsive monoclonal antibodies against extracellular β-d-glucans from Pleurotus ostreatus
Published in Biotechnology progress (01-01-2016)“…β‐d‐glucans from mushroom strains play a major role as biological response modifiers in several clinical disorders. Therefore, a specific assay method is of…”
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Production of hydroxamic acids by immobilized Pseudomonas aeruginosa cells: Kinetic analysis in reverse micelles
Published in Journal of molecular catalysis. B, Enzymatic (01-09-2013)“…•Free and immobilized cells of Pseudomonas aeruginosa L10 contains biocatalyst amidase for hydroxamate derivatives synthesis.•Kinetic studies using a broad…”
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