Pre-concentration by liquid intake by paper (P-CLIP): a new technique for large volumes and digital microfluidics

Microfluidic platforms are an attractive option for incorporating complex fluid handling into low-cost and rapid diagnostic tests. A persistent challenge for microfluidics, however, is the mismatch in the "world-to-chip" interface - it is challenging to detect analytes present at low conce...

Full description

Saved in:

Bibliographic Details
Published in:	Lab on a chip Vol. 17; no. 13; p. 2272
Main Authors:	Rackus, Darius G, de Campos, Richard P S, Chan, Calvin, Karcz, Maria M, Seale, Brendon, Narahari, Tanya, Dixon, Christopher, Chamberlain, M Dean, Wheeler, Aaron R
Format:	Journal Article
Language:	English
Published:	England 01-01-2017
Subjects:	Biomarkers - analysis Humans Immunoassay - instrumentation L-Lactate Dehydrogenase Microfluidic Analytical Techniques - instrumentation Plasmodium falciparum - enzymology Protozoan Proteins Saliva - chemistry
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Microfluidic platforms are an attractive option for incorporating complex fluid handling into low-cost and rapid diagnostic tests. A persistent challenge for microfluidics, however, is the mismatch in the "world-to-chip" interface - it is challenging to detect analytes present at low concentrations in systems that can only handle small volumes of sample. Here we describe a new technique termed pre-concentration by liquid intake by paper (P-CLIP) that addresses this mismatch, allowing digital microfluidics to interface with volumes on the order of hundreds of microliters. In P-CLIP, a virtual microchannel is generated to pass a large volume through the device; analytes captured on magnetic particles can be isolated and then resuspended into smaller volumes for further processing and analysis. We characterize this method and demonstrate its utility with an immunoassay for Plasmodium falciparum lactate dehydrogenase, a malaria biomarker, and propose that the P-CLIP strategy may be useful for a wide range of applications that are currently limited by low-abundance analytes.
ISSN:	1473-0189
DOI:	10.1039/c7lc00440k