Transactivation of interleukin-2 gene via the nuclear proteins from spleen and brain cells

Transactivation of interleukin-2 gene via the nuclear proteins from spleen and brain cells

Immunomodulatory action of nuclear proteins derived from spleen and brain of immunized rats was found in ConA-stimulated mouse spleen lymphocyte cultures. In vitro experiments on 32P- or Dig-labeled promoter-enhancer region (-548 bp) of the human interleukin-2 (IL-2) gene showed specific DNA-nuclear...

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Bibliographic Details
Published in:Biotechnology therapeutics Vol. 4; no. 1-2; p. 63
Main Authors: Kazakova, T B, Golovko, O I, Gushchin, G V, Lebedeva, L V, Dolgi, O D, Karandaschov, E A, Mulberg, A A
Format: Journal Article
Language:English
Published: United States 1993
Subjects:
Animals
Brain - cytology
Brain - metabolism
Cells, Cultured
Concanavalin A - pharmacology
DNA - metabolism
Interleukin-2 - genetics
Molecular Weight
Nuclear Proteins - metabolism
Rats
Rats, Wistar
Regulatory Sequences, Nucleic Acid
Spleen - cytology
Spleen - metabolism
T-Lymphocytes - immunology
T-Lymphocytes - metabolism
Tissue Extracts - pharmacology
Transcriptional Activation
Transfection
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Summary:Immunomodulatory action of nuclear proteins derived from spleen and brain of immunized rats was found in ConA-stimulated mouse spleen lymphocyte cultures. In vitro experiments on 32P- or Dig-labeled promoter-enhancer region (-548 bp) of the human interleukin-2 (IL-2) gene showed specific DNA-nuclear protein complex formation for spleen (6-14 kD) and brain (21 kD) proteins. The functional influence of these trans-factors on the IL-2 promoter region was studied in the model system of Jurkat cells with the firefly luciferase (Luc) reporter gene. The spleen and brain proteins were shown to influence the membrane surface properties of thymic and spleen cells as revealed in a two-phase aqueous system. We conclude that both spleen and brain from immunized rats contain certain protein trans-factors which can be active in T cells and display a common mode of regulatory action the level of IL-2 gene.
ISSN:0898-2848