Gentamicin resistance gene transfer from Enterococcus faecalis and E. faecium to Staphylococcus aureus, S. intermedius and S. hyicus
Transfer of gentamicin resistance from Enterococcus faecalis and E. faecium to Staphylococcus aureus, S. hyicus and S. intermedius was accomplished by filter mating. Transfer from E. faecalis was plasmid mediated and a probe to the gentamicin resistance gene aac(6′)- aph(2″) hybridized to HindIII fr...
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Published in: | Veterinary microbiology Vol. 52; no. 1; pp. 143 - 152 |
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Main Authors: | , , , |
Format: | Journal Article |
Language: | English |
Published: |
Amsterdam
Elsevier B.V
01-09-1996
Elsevier Science |
Subjects: | |
Online Access: | Get full text |
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Summary: | Transfer of gentamicin resistance from
Enterococcus faecalis and
E. faecium to
Staphylococcus aureus, S. hyicus and
S. intermedius was accomplished by filter mating. Transfer from
E. faecalis was plasmid mediated and a probe to the gentamicin resistance gene
aac(6′)-
aph(2″) hybridized to
HindIII fragments of various sizes (7.5, 6.2 and 4.7 kbp) of these plasmids. Transfer from
E. faecium was apparently to the chromosome in 9 of 10 instances with
HindIII fragments of 3.3 or 3.0 kbp hybridizing to the probe. These results are consistent with transposon mediated resistance. Plasmid transfers to
S. intermedius were at much lower frequencies than to
S. aureus or to
S. hyicus but presumed transposon transfer direct to the chromosome was equal in all three species. |
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Bibliography: | L73 9702105 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0378-1135 1873-2542 |
DOI: | 10.1016/0378-1135(96)00057-0 |