Search Results - "Kappen, Lizzy S."

DNA Conformation-Induced Activation of an Enediyne for Site-Specific Cleavage by Kappen, Lizzy S., Goldberg, Irving H.

“…Neocarzinostatin chromophore (NCS chrom) was found to induce site-specific cleavage at the 3′ side of a bulge in single-stranded DNA in the absence of thiol…”
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Probing DNA Bulges with Designed Helical Spirocyclic Molecules by Kappen, Lizzy S, Lin, Yiqing, Jones, Graham B, Goldberg, Irving H

“…Because bulged structures (unpaired bases) in nucleic acids are of general biological significance, it has been of interest to design small molecules as…”
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Development of new simple molecular probes of DNA bulged structures by Xiao, Ziwei, Kappen, Lizzy S., Goldberg, Irving H.

“…NCSi-gb is a neocarzinostatin chromophore metabolite which binds strongly to certain two-base DNA bulges. New strongly fluorescent analogues of NCSi-gb…”
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Isotope effects on the sequence-specific cleavage of DNA by neocarzinostatin: kinetic partitioning between 4'- and 5'-hydrogen abstraction at unique thymidine sites by Frank, Bruce L, Worth, Leroy, Christner, Donna F, Kozarich, John W, Stubbe, JoAnne, Kappen, Lizzy S, Goldberg, Irving H

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Bifunctional involvement of the hydroxynaphthoate ester moiety in the activation of neocarzinostatin chromophore in DNA-mediated site-specific cleavage by Hensens, Otto D, Helms, Gregory L, Zink, Deborah L, Chin, Der Hang, Kappen, Lizzy S, Goldberg, Irving H

“…Thiol reagents activate the enediyne antibiotic neocarzinostatin chromophore (NCS-Chrom, 1) for duplex DNA cleavage by greater than 1000-fold. Nucleophilic…”
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Isotope effects on the sequence-specific cleavage of dC in d(AGC) sequences by neocarzinostatin: elucidation of chemistry of minor lesions by Kappen, Lizzy S, Goldberg, Irving H, Wu, S. H, Stubbe, JoAnne, Worth, Leroy, Kozarich, John W

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Mismatch-induced switch of neocarzinostatin attack sites in the DNA minor groove by Kappen, Lizzy S, Goldberg, Irving H

“…Based on the finding that the wobble G.T mismatch 5' to the C of AGC.GCT results in switching of the attack chemistry by neocarzinostatin chromophore…”
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New Complex of Post-Activated Neocarzinostatin Chromophore with DNA:  Bulge DNA Binding from the Minor Groove by Kwon, Youngjoo, Xi, Zhen, Kappen, Lizzy S, Goldberg, Irving H, Gao, Xiaolian

“…Neocarzinostatin (NCS-chrom), a natural enediyne antitumor antibiotic, undergoes either thiol-dependent or thiol-independent activation, resulting in…”
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Stimulation of DNA Strand Slippage Synthesis by a Bulge Binding Synthetic Agent by Kappen, Lizzy S, Xi, Zhen, Jones, Graham B, Goldberg, Irving H

“…It has been postulated that bulged structures may be intermediates in the DNA strand slippage synthesis associated with the expansion of nucleotide repeats in…”
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Probing DNA Single Strands for Single-Base Bulges with Neocarzinostatin Chromophore by Kappen, Lizzy S, Xi, Zhen, Goldberg, Irving H

“…Neocarzinostatin chromophore (NCS-Chrom) induces strong cleavage at a single site (C3) in the single-stranded and 5‘ 32P-end-labeled 13-mer GCCAGATTTGAGC in a…”
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Bulge-Specific Cleavage in Transactivation Response Region RNA and Its DNA Analog by Neocarzinostatin Chromophore by Kappen, Lizzy S, Goldberg, Irving H

“…On the basis of the finding that in the absence of thiol the nonprotein chromophore of the antitumor drug neocarzinostatin (NCS-chrom) induces highly efficient…”
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Characterization of a Covalent Monoadduct of Neocarzinostatin Chromophore at a DNA Bulge by Kappen, Lizzy S, Goldberg, Irving H

“…Neocarzinostatin chromophore (NCS-Chrom) induces highly efficient site-specific strand cleavage at the bulge of a folded single-stranded 31-mer DNA in the…”
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Identification of 2-deoxyribonolactone at the site of neocarzinostatin-induced cytosine release in the sequence d(AGC) by Kappen, Lizzy S, Goldberg, Irving H

“…Neocarzinostatin- (NCS) induced release of cytosine from the deoxycytidylate residues of d(AGC) sequences of duplex oligonucleotides leaves a damaged sugar…”
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Replication Block by an Enediyne Drug−DNA Deoxyribose Adduct by Kappen, Lizzy S, Goldberg, Irving H

“…Under anaerobic conditions neocarzinostatin chromophore, an enediyne antibiotic, forms a covalent drug−DNA adduct on the 5‘ carbon of deoxyribose at a specific…”
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Neocarzinostatin Acts as a Sensitive Probe of DNA Microheterogeneity: Switching of Chemistry from C-1' to C-4' by a G·T Mismatch 5' to the Site of DNA Damage by Kappen, Lizzy S., Goldberg, Irving H.

“…The diradical form of thiol-activated neocarzinostatin chromophore resides in the minor groove of DNA, where it has access to hydrogen atoms at the C-5', C-1',…”
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Neocarzinostatin-induced hydrogen atom abstraction from C-4' and C-5' of the T residue at a d(GT) step in oligonucleotides: shuttling between deoxyribose attack sites based on isotope selection effects by Kappen, Lizzy S, Goldberg, Irving H, Frank, Bruce L, Worth, Leroy, Christner, Donna F, Kozarich, John W, Stubbe, JoAnne

“…The thiol-activated neocarzinostatin chromophore cleaves duplex oligonucleotides containing the sequence-TGTTTGA-, producing 3'-phosphoglycolate and…”
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Double-Stranded Damage of DNA.cntdot.RNA Hybrids by Neocarzinostatin Chromophore: Selective C-1' Chemistry on the RNA Strand by Zeng, Xiaoping, Xi, Zhen, Kappen, Lizzy S, Tan, Weitian, Goldberg, Irving H

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Deoxyribonucleic acid damage by neocarzinostatin chromophore: strand breaks generated by selective oxidation of C-5' of deoxyribose by Kappen, Lizzy S, Goldberg, Irving H

“…Among the lesions induced in DNA by neocarzinostatin chromophore are spontaneous and alkali-dependent base release, sugar damage, and single-strand breaks with…”
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Stabilization of neocarzinostatin nonprotein chromophore activity by interaction with apoprotein and with HeLa cells by Kappen, Lizzy S, Goldberg, Irving H

“…The methanol-extracted, nonprotein chromophore of the protein antibiotic neocarzinostatin (NCS), which possesses the full in vitro and in vivo deoxyribonucleic…”
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Mechanism and base specificity of DNA breakage in intact cells by neocarzinostatin by Kappen, Lizzy S, Ellenberger, Thomas E, Goldberg, Irving H

“…When electrophoresed on an agarose gel, the DNA isolated from neocarzinostatin- (NCS-) treated HeLa cells migrates in a ladder of discrete bands indicative of…”
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