Search Results - "Kaplan, Daniel L"

Conserved mechanism for coordinating replication fork helicase assembly with phosphorylation of the helicase by Bruck, Irina, Kaplan, Daniel L.

“…Dbf4-dependent kinase (DDK) phosphorylates minichromosome maintenance 2 (Mcm2) during S phase in yeast, and Sld3 recruits cell division cycle 45 (Cdc45) to…”
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The Dbf4-Cdc7 Kinase Promotes Mcm2-7 Ring Opening to Allow for Single-stranded DNA Extrusion and Helicase Assembly by Bruck, Irina, Kaplan, Daniel L.

“…The replication fork helicase in eukaryotes is composed of Cdc45, Mcm2-7, and GINS (CMG). The Dbf4-Cdc7 kinase phosphorylates Mcm2 in vitro, but the in vivo…”
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DNA Sequences Proximal to Human Mitochondrial DNA Deletion Breakpoints Prevalent in Human Disease Form G-quadruplexes, a Class of DNA Structures Inefficiently Unwound by the Mitochondrial Replicative Twinkle Helicase by Bharti, Sanjay Kumar, Sommers, Joshua A., Zhou, Jun, Kaplan, Daniel L., Spelbrink, Johannes N., Mergny, Jean-Louis, Brosh, Robert M.

“…Mitochondrial DNA deletions are prominent in human genetic disorders, cancer, and aging. It is thought that stalling of the mitochondrial replication machinery…”
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Mcm10 coordinates the timely assembly and activation of the replication fork helicase by Perez-Arnaiz, Patricia, Bruck, Irina, Kaplan, Daniel L

“…Mcm10 is an essential replication factor that is required for DNA replication in eukaryotes. Two key steps in the initiation of DNA replication are the…”
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Cdc45 Protein-Single-stranded DNA Interaction Is Important for Stalling the Helicase during Replication Stress by Bruck, Irina, Kaplan, Daniel L.

“…Replicative polymerase stalling is coordinated with replicative helicase stalling in eukaryotes, but the mechanism underlying this coordination is not known…”
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Impact of age-associated cyclopurine lesions on DNA repair helicases by Khan, Irfan, Suhasini, Avvaru N, Banerjee, Taraswi, Sommers, Joshua A, Kaplan, Daniel L, Kuper, Jochen, Kisker, Caroline, Brosh, Jr, Robert M

“…8,5' cyclopurine deoxynucleosides (cPu) are locally distorting DNA base lesions corrected by nucleotide excision repair (NER) and proposed to play a role in…”
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Dpb11 may function with RPA and DNA to initiate DNA replication by Bruck, Irina, Dhingra, Nalini, Martinez, Matthew P, Kaplan, Daniel L

“…Dpb11 is required for the initiation of DNA replication in budding yeast. We found that Dpb11 binds tightly to single-stranded DNA (ssDNA) or branched DNA…”
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Mcm4,6,7 Uses a “Pump in Ring” Mechanism to Unwind DNA by Steric Exclusion and Actively Translocate along a Duplex by Kaplan, Daniel L., Davey, Megan J., O'Donnell, Mike

“…Mcm4,6,7 is a ring-shaped heterohexamer and the putative eukaryotic replication fork helicase. In this study, we examine the mechanism of Mcm4,6,7. Mcm4,6,7…”
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Mechanisms of polar arrest of a replication fork by Kaplan, Daniel L, Bastia, Deepak

“…A DNA replication terminator sequence blocks an approaching replication fork when the moving replisome approaches from just one direction. The mechanism…”
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DnaB Drives DNA Branch Migration and Dislodges Proteins While Encircling Two DNA Strands by Kaplan, Daniel L., O'Donnell, Mike

“…DnaB is a ring-shaped, hexameric helicase that unwinds the E. coli DNA replication fork while encircling one DNA strand. This report demonstrates that DnaB can…”
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Mcm Subunits Can Assemble into Two Different Active Unwinding Complexes by Kanter, Diane M., Bruck, Irina, Kaplan, Daniel L.

“…The replication fork helicase in eukaryotes is a large complex that is composed of Mcm2-7, Cdc45, and GINS. The Mcm2-7 proteins form a heterohexameric ring…”
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Twin DNA Pumps of a Hexameric Helicase Provide Power to Simultaneously Melt Two Duplexes by Kaplan, Daniel L, O'Donnell, Mike

“…DnaB is the primary replicative helicase in Escherichia coli. We show here that DnaB can unwind two duplex arms simultaneously for an extended distance…”
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An Mcm10 Mutant Defective in ssDNA Binding Shows Defects in DNA Replication Initiation by Perez-Arnaiz, Patricia, Kaplan, Daniel L.

“…Mcm10 is an essential protein that functions to initiate DNA replication after the formation of the replication fork helicase. In this manuscript, we…”
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The Replication Initiation Protein Sld3/Treslin Orchestrates the Assembly of the Replication Fork Helicase during S Phase by Bruck, Irina, Kaplan, Daniel L.

“…The initiation of DNA replication is a highly regulated process in eukaryotic cells, and central to the process of initiation is the assembly and activation of…”
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A Positive Amplification Mechanism Involving a Kinase and Replication Initiation Factor Helps Assemble the Replication Fork Helicase by Bruck, Irina, Dhingra, Nalini, Kaplan, Daniel L

“…The assembly of the replication fork helicase during S phase is key to the initiation of DNA replication in eukaryotic cells. One step in this assembly in…”
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The Dbf4-Cdc7 kinase promotes Mcm2-7 ring opening to allow for single-stranded DNA extrusion and helicase assembly by Bruck, Irina, Kaplan, Daniel L.

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The replication initiation protein Sld3/Treslin orchestrates the assembly of the replication fork helicase during S phase by Bruck, Irina, Kaplan, Daniel L.

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Origin single-stranded DNA releases Sld3 protein from the Mcm2–7 complex, allowing the GINS tetramer to bind the Mcm2–7 complex by Bruck, Irina, Kaplan, Daniel L.

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The replication initiation protein Sld2 regulates helicase assembly by Bruck, Irina, Kaplan, Daniel L

“…Assembly of the Cdc45-Mcm2-7-GINS (CMG) replicative helicase complex must be regulated to ensure that DNA unwinding is coupled with DNA synthesis. Sld2 is…”
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Dpb11 Protein Helps Control Assembly of the Cdc45·Mcm2-7·GINS Replication Fork Helicase by Dhingra, Nalini, Bruck, Irina, Smith, Skye, Ning, Boting, Kaplan, Daniel L.

“…Dpb11 is required for the initiation of DNA replication in budding yeast. Dpb11 binds to S-phase cyclin-dependent kinase-phosphorylated Sld2 and Sld3 to form a…”
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