Search Results - "Kandula, Sunitha"

Accurate and Rapid Protein Concentration Measurement of In‐Process, High Concentration Protein Pools by McKechnie, William S., Tugcu, Nihal, Kandula, Sunitha

“…Protein concentration is a critical product quality attribute and required for any therapeutic protein. Many commercial and investigational new biologics are…”
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Impact of Freeze/Thaw Process on Drug Substance Storage of Therapeutics by Rayfield, William J, Kandula, Sunitha, Khan, Heera, Tugcu, Nihal

“…The storage of drug substance at subzero temperatures mitigates potential risks associated with liquid storage, such as degradation and shipping stress, making…”
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Defining process design space for monoclonal antibody cell culture by Abu-Absi, Susan Fugett, Yang, LiYing, Thompson, Patrick, Jiang, Canping, Kandula, Sunitha, Schilling, Bernhard, Shukla, Abhinav A

“…The concept of design space has been taking root as a foundation of in-process control strategies for biopharmaceutical manufacturing processes. During mapping…”
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A high‐throughput approach to developing and optimizing mixed‐mode membrane chromatography for protein purification by McKechnie, William S., Thom, Volkmar, Kupracz, Lukas, Pollard, Jennifer, Kandula, Sunitha, Welsh, John

“…Membrane chromatography has been established as a viable alternative to packed‐bed column chromatography for the purification of therapeutic proteins…”
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Cation exchange as a single polishing step for conjugated peptides by Rockwell, Lauren, Bao, Haiying, Insaidoo, Francis, Ikechukwu, Ijeoma, Tugcu, Nihal, Kandula, Sunitha

“…Purification of peptides typically includes expensive reverse phase (RP) processes, which utilize high pressure and large volumes of solvent. For two…”
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Cationic polymer precipitation for enhanced impurity removal in downstream processing by Li, Zhao, Chen, Justin, Martinez‐Fonts, Kirby, Rauscher, Michael, Rivera, Shannon, Welsh, John, Kandula, Sunitha

“…Precipitation can be used for the removal of impurities early in the downstream purification process of biologics, with the soluble product remaining in the…”
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High throughput chromatography strategies for potential use in the formal process characterization of a monoclonal antibody by Petroff, Matthew G., Bao, Haiying, Welsh, John P., van Beuningen - de Vaan, Miranda, Pollard, Jennifer M., Roush, David J., Kandula, Sunitha, Machielsen, Peter, Tugcu, Nihal, Linden, Thomas O.

“…ABSTRACT High throughput experimental strategies are central to the rapid optimization of biologics purification processes. In this work, we extend common high…”
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Minor Thermostable Alkaline Protease Produced by Thennoactinomyces sp. E79 by Young Ok Kim, Jung Kee Lee, Kandula Sunitha, Hyung Kwoun Kim, Tae Kwang Oh

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Design of a filter train for precipitate removal in monoclonal antibody downstream processing by Kandula, Sunitha, Babu, Sudha, Jin, Mi, Shukla, Abhinav A.

“…Protein A chromatography has become widely established for the preparative purification of mAbs (monoclonal antibodies). Low pH elution from Protein A columns…”
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Refolding and Purification of Zymomonas mobilis Levansucrase Produced as Inclusion Bodies in Fed-Batch Culture of Recombinant Escherichia coli by Sunitha, Kandula, Chung, Bong Hyun, Jang, Ki-Hyo, Song, Ki-Bang, Kim, Chul Ho, Rhee, Sang-Ki

“…Zymomonas mobilis levansucrase was overproduced by the fed-batch culture of recombinant Escherichia coli harboring a novel expression system that is…”
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Minor thermostable alkaline protease produced by Thermoactinomyces sp. E79 by YOUNG-OK, K, LEE, J.-K, SUNITHA, K, KIM, H.-K, OH, T.-K

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