Restoration of the growth of Escherichia coli under K+-deficient conditions by Cs+ incorporation via the K+ transporter Kup

Biological incorporation of cesium ions (Cs + ) has recently attracted significant attention in terms of the possible applications for bioremediation of radiocesium and their significant roles in biogeochemical cycling. Although high concentrations of Cs + exhibit cytotoxicity on microorganisms, the...

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Published in:	Scientific reports Vol. 7; no. 1; pp. 1965 - 6
Main Authors:	Kato, Souichiro, Kanata, Yoshiki, Kitagawa, Wataru, Sone, Teruo, Asano, Kozo, Kamagata, Yoichi
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 16-05-2017 Nature Publishing Group Nature Portfolio
Subjects:	38/90 42 42/44 631/326/41/1969 631/45/269/1151 Biogeochemical cycles Biological Transport Bioremediation Cesium Cesium - metabolism Cesium - toxicity Cytotoxicity E coli Escherichia coli Escherichia coli - drug effects Escherichia coli - physiology Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Gene Expression Regulation, Bacterial Humanities and Social Sciences Incorporation Intracellular Microorganisms multidisciplinary Mutation Potassium Potassium - metabolism Potassium Deficiency - metabolism Science Science (multidisciplinary) Strains (organisms)
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Summary:	Biological incorporation of cesium ions (Cs + ) has recently attracted significant attention in terms of the possible applications for bioremediation of radiocesium and their significant roles in biogeochemical cycling. Although high concentrations of Cs + exhibit cytotoxicity on microorganisms, there are a few reports on the promotive effects of Cs + on microbial growth under K + -deficient conditions. However, whether this growth-promoting effect is a common phenomenon remains uncertain, and direct correlation between growth promotion and Cs + uptake abilities has not been confirmed yet. Here, we validated the growth promotive effects of Cs + uptake under K + -deficient conditions using an Escherichia coli strain with an inducible expression of the Kup K + transporter that has nonspecific Cs + transport activities (strain kup -IE). The strain kup -IE exhibited superior growth under the Cs + -supplemented and K + -deficient conditions compared to the wild type and the kup null strains. The intracellular Cs + levels were significantly higher in strain kup -IE than in the other strains, and were well correlated with their growth yields. Furthermore, induction levels of the kup gene, intracellular Cs + concentrations, and the growth stimulation by Cs + also correlated positively. These results clearly demonstrated that Cs + incorporation via Kup transporter restores growth defects of E. coli under K + -deficient conditions.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-017-02024-4