Association complexes between ovalbumin and cyclodextrins have no effect on the immunological properties of ovalbumin

Delivery systems are designed to deliver necessary amounts of drugs without modifying their biological features. Cyclodextrins (CD) are potential candidates for such a role due to their ability to alter physical and chemical properties of guest molecules by the formation of inclusion/association com...

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Bibliographic Details
Published in:	European journal of pharmaceutics and biopharmaceutics Vol. 57; no. 2; pp. 199 - 205
Main Authors:	Kamphorst, A.O, Mendes de Sá, I, Faria, A.M.C, Sinisterra, R.D
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-03-2004
Subjects:	Administration, Oral Animals Antigenicity Antigens - administration & dosage Antigens - immunology Cells, Cultured Cyclodextrin Cyclodextrins - administration & dosage Cyclodextrins - immunology Drug Delivery Systems - methods Immunization Injections, Subcutaneous Mice Mice, Inbred C57BL Mice, Inbred DBA Oral tolerance Ovalbumin Ovalbumin - administration & dosage Ovalbumin - immunology Spleen - drug effects Spleen - immunology Ovalbumin DTG, first derivative of thermogravimetry curve DSC, differential scanning calorimetry Immunization Ova, ovalbumin TG, thermogravimetry curve Oral tolerance ELISA, enzyme-linked immunosorbent assay XRD, X-ray pattern diffraction Cyclodextrin CD, cyclodextrins Antigenicity i.p., intraperitoneal s.c., subcutaneous FTIR, Fourier transform infrared spectroscopy CFA, complete Freund's adjuvant
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Summary:	Delivery systems are designed to deliver necessary amounts of drugs without modifying their biological features. Cyclodextrins (CD) are potential candidates for such a role due to their ability to alter physical and chemical properties of guest molecules by the formation of inclusion/association complexes. They have already been used to stabilize and solubilize peptides and proteins of biological importance. However, no systematic study has been reported on their immunological effects upon coupling to such proteins. Herein, we prepared and characterized the association complexes between α-, β- and hydroxypropyl-β-cyclodextrin and ovalbumin (Ova). Afterward we tested the effect of CD coupling in the Ova antigenicity and the immunological effects of CD coupling on Ova oral and subcutaneous administration. Our results clearly show that CD-coupled Ova elicits the same immunological activities as uncoupled Ova. Therefore, we conclude that CDs are immunologically safe for use as delivery systems in animals.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0939-6411 1873-3441
DOI:	10.1016/j.ejpb.2003.10.019