Search Results - "Kalies, Stefan"

Femtosecond laser-based nanosurgery reveals the endogenous regeneration of single Z-discs including physiological consequences for cardiomyocytes by Müller, Dominik, Hagenah, Dorian, Biswanath, Santoshi, Coffee, Michelle, Kampmann, Andreas, Zweigerdt, Robert, Heisterkamp, Alexander, Kalies, Stefan M. K.

“…A highly organized cytoskeleton architecture is the basis for continuous and controlled contraction in cardiomyocytes (CMs). Abnormalities in cytoskeletal…”
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Longitudinal imaging and femtosecond laser manipulation of the liver: How to generate and trace single-cell-resolved micro-damage in vivo by DeTemple, Daphne E, Cammann, Sebastian, Bahlmann, Julia, Buettner, Manuela, Heisterkamp, Alexander, Vondran, Florian W R, Kalies, Stefan K

“…The liver is known to possess extensive regenerative capabilities, the processes and pathways of which are not fully understood. A necessary step towards a…”
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Formation of three-dimensional tubular endothelial cell networks under defined serum-free cell culture conditions in human collagen hydrogels by Andrée, Birgit, Ichanti, Houda, Kalies, Stefan, Heisterkamp, Alexander, Strauß, Sarah, Vogt, Peter-Maria, Haverich, Axel, Hilfiker, Andres

“…Implementation of tubular endothelial cell networks is a prerequisite for 3D tissue engineering of constructs with clinically relevant size as nourishment of…”
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Acoustic stimulation of the human round window by laser-induced nonlinear optoacoustics by Lengert, Liza, Tomanek, Michael, Ghoncheh, Mohammad, Lohmann, Hinnerk, Prenzler, Nils, Kalies, Stefan, Johannsmeier, Sonja, Ripken, Tammo, Heisterkamp, Alexander, Maier, Hannes

“…The feasibility of low frequency pure tone generation in the inner ear by laser-induced nonlinear optoacoustic effect at the round window was demonstrated in…”
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Gold nanoparticle mediated laser transfection for efficient siRNA mediated gene knock down by Heinemann, Dag, Schomaker, Markus, Kalies, Stefan, Schieck, Maximilian, Carlson, Regina, Murua Escobar, Hugo, Ripken, Tammo, Meyer, Heiko, Heisterkamp, Alexander

“…Laser based transfection methods have proven to be an efficient and gentle alternative to established molecule delivery methods like lipofection or…”
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Parameters for Optoperforation-Induced Killing of Cancer Cells Using Gold Nanoparticles Functionalized With the C-terminal Fragment of Clostridium Perfringens Enterotoxin by Becker, Annegret, Lehrich, Tina, Kalies, Stefan, Heisterkamp, Alexander, Ngezahayo, Anaclet

“…Recently, we used a recombinant produced C-terminus (D194-F319) of the enterotoxin (C-CPE) to functionalize gold nanoparticles (AuNPs) for a subsequent…”
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High density bioprocessing of human pluripotent stem cells by metabolic control and in silico modeling by Manstein, Felix, Ullmann, Kevin, Kropp, Christina, Halloin, Caroline, Triebert, Wiebke, Franke, Annika, Farr, Clara‐Milena, Sahabian, Anais, Haase, Alexandra, Breitkreuz, Yannik, Peitz, Michael, Brüstle, Oliver, Kalies, Stefan, Martin, Ulrich, Olmer, Ruth, Zweigerdt, Robert

“…To harness the full potential of human pluripotent stem cells (hPSCs) we combined instrumented stirred tank bioreactor (STBR) technology with the power of in…”
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Biodistribution, biocompatibility and targeted accumulation of magnetic nanoporous silica nanoparticles as drug carrier in orthopedics by Janßen, Hilke Catherina, Angrisani, Nina, Kalies, Stefan, Hansmann, Florian, Kietzmann, Manfred, Warwas, Dawid Peter, Behrens, Peter, Reifenrath, Janin

“…In orthopedics, the treatment of implant-associated infections represents a high challenge. Especially, potent antibacterial effects at implant surfaces can…”
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Characterization of Tissue Engineered Endothelial Cell Networks in Composite Collagen-Agarose Hydrogels by Ichanti, Houda, Sladic, Sanja, Kalies, Stefan, Haverich, Axel, Andrée, Birgit, Hilfiker, Andres

“…Scaffolds constitute an important element in vascularized tissues and are therefore investigated for providing the desired mechanical stability and enabling…”
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Mimicking acute airway tissue damage using femtosecond laser nanosurgery in airway organoids by Gentemann, Lara, Donath, Sören, Seidler, Anna E., Patyk, Lara, Buettner, Manuela, Heisterkamp, Alexander, Kalies, Stefan

“…Airway organoids derived from adult murine epithelial cells represent a complex 3D in vitro system mimicking the airway epithelial tissue’s native cell…”
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Non-Invasive Full Rheological Characterization via Combined Speckle and Brillouin Microscopy by Wenzel, Johannes, Ruprich, Niklas, Sperlich, Karsten, Stachs, Oliver, Schunemann, Melanie, Leyh, Colette, Kalies, Stefan, Heisterkamp, Alexander

“…Rheology serves to measure the deformation and flow of materials. Its associated quantities, for example, the Young's modulus, shear modulus, bulk modulus, or…”
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Characterization of nanoparticle mediated laser transfection by femtosecond laser pulses for applications in molecular medicine by Schomaker, Markus, Heinemann, Dag, Kalies, Stefan, Willenbrock, Saskia, Wagner, Siegfried, Nolte, Ingo, Ripken, Tammo, Murua Escobar, Hugo, Meyer, Heiko, Heisterkamp, Alexander

“…In molecular medicine, the manipulation of cells is prerequisite to evaluate genes as therapeutic targets or to transfect cells to develop cell therapeutic…”
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Investigation of biophysical mechanisms in gold nanoparticle mediated laser manipulation of cells using a multimodal holographic and fluorescence imaging setup by Kalies, Stefan, Antonopoulos, Georgios C, Rakoski, Mirko S, Heinemann, Dag, Schomaker, Markus, Ripken, Tammo, Meyer, Heiko

“…Laser based cell manipulation has proven to be a versatile tool in biomedical applications. In this context, combining weakly focused laser pulses and…”
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Enhancement of extracellular molecule uptake in plasmonic laser perforation by Kalies, Stefan, Birr, Tobias, Heinemann, Dag, Schomaker, Markus, Ripken, Tammo, Heisterkamp, Alexander, Meyer, Heiko

“…The use of laser induced surface plasmons on metal nanoparticles has proven to be an excellent tool for the delivery of molecules like siRNA and DNA into…”
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How Localized Z-Disc Damage Affects Force Generation and Gene Expression in Cardiomyocytes by Müller, Dominik, Donath, Sören, Brückner, Emanuel Georg, Biswanath Devadas, Santoshi, Daniel, Fiene, Gentemann, Lara, Zweigerdt, Robert, Heisterkamp, Alexander, Kalies, Stefan Michael Klaus

“…The proper function of cardiomyocytes (CMs) is highly related to the Z-disc, which has a pivotal role in orchestrating the sarcomeric cytoskeletal function. To…”
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Epithelial restitution in 3D - Revealing biomechanical and physiochemical dynamics in intestinal organoids via fs laser nanosurgery by Donath, Sören, Seidler, Anna Elisabeth, Mundin, Karlina, Wenzel, Johannes, Scholz, Jonas, Gentemann, Lara, Kalies, Julia, Faix, Jan, Ngezahayo, Anaclet, Bleich, André, Heisterkamp, Alexander, Buettner, Manuela, Kalies, Stefan

“…Intestinal organoids represent a three-dimensional cell culture system mimicking the mammalian intestine. The application of single-cell ablation for defined…”
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Probing interneuronal cell communication via optogenetic stimulation by Heeger, Patrick, Harre, Jennifer, Warnecke, Athanasia, Mueller, Dominik, Kalies, Stefan, Heisterkamp, Alexander

“…This study uses an all‐optical approach to probe interneuronal communication between spiral ganglion neurons (SGNs) and neurons of other functional units, in…”
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Balanced Heterodyne Brillouin Spectroscopy Towards Tissue Characterization by Schunemann, Melanie, Sperlich, Karsten, Barnscheidt, Kai, Schopa, Samuel, Wenzel, Johannes, Kalies, Stefan, Heisterkamp, Alexander, Stolz, Heinrich, Stachs, Oliver, Hage, Boris

“…We present the first application of balanced heterodyne detection, established in quantum optics, to Brillouin spectroscopy in biological tissues. The balanced…”
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Intestinal Organoids in Colitis Research: Focusing on Variability and Cryopreservation by zur Bruegge, Talke F., Liese, Andrea, Donath, Sören, Kalies, Stefan, Kosanke, Maike, Dittrich-Breiholz, Oliver, Czech, Sandra, Bauer, Verena N., Bleich, André, Buettner, Manuela

“…In recent years, stem cell-derived organoids have become a cell culture standard that is widely used for studying various scientific issues that were…”
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CD14 and ALPK1 Affect Expression of Tight Junction Components and Proinflammatory Mediators upon Bacterial Stimulation in a Colonic 3D Organoid Model by Buettner, Manuela, Liese, Andrea, Villarreal, Santiago Nahuel, Kalies, Stefan, Boyle, Erin C., zur Bruegge, Talke, Brooks, Pascal, Bleich, André

“…Cd14 and Alpk1 both encode pathogen recognition receptors and are known candidate genes for affecting severity in inflammatory bowel diseases. CD14 acts as a…”
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