Creatinine-Iron Complex and Its Use in Electrochemical Measurement of Urine Creatinine

A non-enzymatic electrochemical technique for creatinine sensing is presented, exploiting iron binding property of creatinine. Disposable carbon printed electrodes layered with FeCl 3 coated cotton fiber membranes are used to sense creatinine from 10 to 245 mg/dl, on clinical urine samples. The ener...

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Bibliographic Details
Published in:	IEEE sensors journal Vol. 18; no. 2; pp. 830 - 836
Main Authors:	Kumar, Vinay, Hebbar, Suraj, Kalam, Rahila, Panwar, Sachin, Prasad, Sujay, Srikanta, S. S., Krishnaswamy, P. R., Bhat, Navakanta
Format:	Journal Article
Language:	English
Published:	IEEE 15-01-2018
Subjects:	ACR Biosensors Carbon chrono-amperometry Cotton Creatinine-iron binding disposable test strips electrochemical sensor Electrodes microalbuminuria interference point of care device Strips
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Summary:	A non-enzymatic electrochemical technique for creatinine sensing is presented, exploiting iron binding property of creatinine. Disposable carbon printed electrodes layered with FeCl 3 coated cotton fiber membranes are used to sense creatinine from 10 to 245 mg/dl, on clinical urine samples. The energydispersive X-ray spectroscopy analysis confirms the presence of Fe(III) dry chemistry on cotton membrane. Creatinine binding with Fe(III) is verified with UV analysis, with a corresponding decrease in Fe(III) reduction current in cyclic voltammetry. The disposable test strips are interfaced with multi-potentiostat point of care (POC) hand-held device, working in amperometry mode. The results obtained on POC biosensors demonstrate good correlation (R 2 = 0.91) with Jaffe method laboratory gold standard. The intra-assay variability is less than 7.1%. The statistical bias as revealed from the Bland-Altman analysis indicates that the POC results are within 95% confidence interval. This POC device does not require any sample preparation step and provides sample to result in less than a minute. FeCl 3 sensing chemistry is robust against urine albumin interference, which is especially significant for accurate estimation of albumin to creatinine ratio. The non-enzymatic nature of disposable test strips results in highly stable and robust operation of the POC device over a large range of temperature variations.
ISSN:	1530-437X 1558-1748
DOI:	10.1109/JSEN.2017.2777913