Murine embryos as a direct target for some human autoantibodies in vitro

The involvement of one or another autoantibody in reproductive failure have long been thought to be through post-implantation thrombosis and/or peri-implantation trophoblast dysfunction and/or maternal hormonal imbalance. It can be postulated that the embryo may be a direct target for some autoantib...

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Bibliographic Details
Published in:	Human reproduction (Oxford) Vol. 14; no. 10; pp. 2556 - 2561
Main Authors:	Kaider, B.D., Coulam, C.B., Roussev, R.G.
Format:	Journal Article
Language:	English
Published:	Oxford Oxford University Press 01-10-1999
Subjects:	Animals Antibody Specificity Antigen-Antibody Reactions antiphospholipids autoantibodies Autoantibodies - immunology Biological and medical sciences Culture Techniques Embryo Implantation - immunology Embryo, Mammalian - immunology Embryology: invertebrates and vertebrates. Teratology embryotoxicity Female Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology General aspects General aspects. Development. Fetal membranes human Humans Immunoglobulins - immunology Mice mouse embryos embryotoxicity mouse embryos autoantibodies human antiphospholipids Human Embryonic development Embryo Toxicity Rodentia Autoantibody Phospholipid In vitro Vertebrata Mammalia Mouse Embryo culture Immunofluorescence
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Summary:	The involvement of one or another autoantibody in reproductive failure have long been thought to be through post-implantation thrombosis and/or peri-implantation trophoblast dysfunction and/or maternal hormonal imbalance. It can be postulated that the embryo may be a direct target for some autoantibodies prior to implantation. Mouse embryos have been labelled and cultured with affinity purified immunoglobulin (IgG) and IgA from positive for antiphospholipid antibody sera, as well as IgG from positive for antinuclear antibody sera and positive for antithyroid antibody sera. Intact IgG and IgA from healthy individuals were used as controls. All embryos cultured with purified antiphospholipid IgG or IgA, and anti-nuclear IgG exhibited strong immunofluorescence. No difference in fluorescent intensity was observed whether antiphospholipid or anti-nuclear antibodies were used, but the pattern of antibody distribution seemed to be different. Antiphospholipid IgG was more dominant on the zona pellucida, while antiphospholipid IgA and antinuclear IgG had predominant distribution on the embryonic cells. None of the embryos cultured with antithyroid IgG or with control immunoglobulins showed strong immunofluorescence. Embryos cultured with purified antiphospholipid and antinuclear immunoglobulins experienced significant growth impairment or death compared to those cultured with antithyroid or control immunoglobulins.
Bibliography:	local:0142556 ark:/67375/HXZ-01NN062N-4 istex:28AC411AB1E82EA3EEC1651F4E4BD4AD42146B40 PII:1460-2350 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0268-1161 1460-2350
DOI:	10.1093/humrep/14.10.2556