Respiratory mechanics do not always mirror pulmonary histological changes in emphysema

: To verify the accordance of functional and morphometric parameters during the development of emphysema. : BALB/c mice received a nasal drop of either papain or saline solution and were studied after 1, 3, 15, 28, and 40 days. Functional parameters, such as airway resistance, tissue damping, and ti...

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Bibliographic Details
Published in:	Clinics (São Paulo, Brazil) Vol. 66; no. 10; pp. 1797 - 1803
Main Authors:	Anciães, Adriana Martins, Olivo, Clarice Rosa, Prado, Carla Máximo, Kagohara, Keila H, da Silva Pinto, Tatiana, Moriya, Henrique T, Mauad, Thais, de Arruda Martins, Mílton, dos Santos Lopes, Fernanda Degobbi Tenorio Quirino
Format:	Journal Article
Language:	English
Published:	United States Elsevier España, S.L.U 01-01-2011 Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo Faculdade de Medicina / USP Elsevier España
Subjects:	Animal model Animals Basic Research Collagen - metabolism Disease Models, Animal Disease Progression Elastic Tissue - metabolism Emphysema Immunohistochemistry Lung - pathology Lung - physiopathology Macrophages - metabolism Male Matrix Metalloproteinase 12 - metabolism Mean linear intercept MEDICINE, GENERAL & INTERNAL Mice Mice, Inbred BALB C Papain Parenchyma remodeling Pulmonary Emphysema - chemically induced Pulmonary Emphysema - pathology Pulmonary Emphysema - physiopathology Respiratory mechanics Respiratory Mechanics - physiology Time Factors Animal model Respiratory mechanics Emphysema Parenchyma remodeling Mean linear intercept
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Summary:	: To verify the accordance of functional and morphometric parameters during the development of emphysema. : BALB/c mice received a nasal drop of either papain or saline solution and were studied after 1, 3, 15, 28, and 40 days. Functional parameters, such as airway resistance, tissue damping, and tissue elastance, were analyzed. To evaluate the structural changes and possible mechanisms involved in this disease, we measured the mean linear intercept, the volume proportions of elastic and collagen fibers, the number of macrophages, the numbers of cells expressing metalloprotease 12 and 8-isoprostane in lung parenchyma. : We only observed decreases in tissue elastance and tissue damping on the 28th day, with a concomitant increase in the mean linear intercept, indicating the presence of emphysema. However, only the mean linear intercept values remained increased until the 40th day. The volume proportion of collagen fibers was increased from the 15th day to the 40th day, whereas the volume proportion of elastic fibers was only increased on the 40th day. The number of macrophages increased beginning on the 1st day. The expression of metalloproteinase 12 was increased from the 3rd day until the 40th day. However, 8-isoprostane expression was only increased on the 1st and 3rd days. : In this study, morphometric parameters were found to be more reliable for detecting the presence of emphysema than the functional parameters measured by respiratory mechanics. Further investigations are necessary to understand how the extracellular matrix remodeling observed in the lung parenchyma could be involved in this process.
ISSN:	1807-5932 1980-5322 1980-5322
DOI:	10.1590/S1807-59322011001000020