Antibodies recognizing CD24 LAP epitope on human T cells enhance CD28 and IL-2 T cell proliferation

Membrane expression of the CD24 molecule on activated T lymphocytes is not elucidated fully. We previously described the intracellular and cell‐surface expression of the CD24 sialic acid‐dependent epitope(s) on phytohemagglutinin‐activated peripheral blood mononuclear cells. However, the CD24 core p...

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Bibliographic Details
Published in:	Journal of leukocyte biology Vol. 69; no. 2; pp. 215 - 223
Main Authors:	C. Salamone, María, Rosselot, Carolina, Salamone, Gabriela V., Barboza, Marcos, Kado, Miguel, Fainboim, Leonardo
Format:	Journal Article
Language:	English
Published:	United States Society for Leukocyte Biology 01-02-2001
Subjects:	Adjuvants, Immunologic - metabolism Adjuvants, Immunologic - pharmacology Alanine Antibodies, Monoclonal - metabolism Antibodies, Monoclonal - pharmacology Antigens, CD - immunology CD24 Antigen CD28 antigen CD28 Antigens - physiology CD4-Positive T-Lymphocytes - immunology CD4-Positive T-Lymphocytes - metabolism CD8-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - metabolism Cell Membrane - immunology Cell Membrane - metabolism Cells, Cultured costimulation signals Epitopes, T-Lymphocyte - immunology Epitopes, T-Lymphocyte - metabolism Humans Interleukin-2 - physiology Interphase - immunology Leucine Leukocytes, Mononuclear - immunology Lymphocyte Activation - immunology Membrane Glycoproteins Oligopeptides - immunology Oligopeptides - metabolism PBMC phytohemagglutinin Proline T-Lymphocyte Subsets - immunology T-Lymphocyte Subsets - metabolism
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Summary:	Membrane expression of the CD24 molecule on activated T lymphocytes is not elucidated fully. We previously described the intracellular and cell‐surface expression of the CD24 sialic acid‐dependent epitope(s) on phytohemagglutinin‐activated peripheral blood mononuclear cells. However, the CD24 core protein was not detected previously on human T cells. This study reinvestigated the expression and role of CD24 in T cell subsets. We analyzed binding of anti‐CD24 monoclonal antibodies (mAbs) to sialic and leucine‐alanine‐proline (LAP) epitopes in resting and activated, normal T lymphocytes. CD24 LAP and CD24 sialic epitopes were detected on activated CD4‐ and CD8‐positive cells. Although expression of CD24 sialic epitopes remained stably expressed in interleukin (IL)‐2‐dependent cultures, T cell expression of the LAP epitope was transient. Anti‐LAP antibodies strongly enhanced the response of T cells to a combination of anti‐CD3/CD28 mAbs and enhanced proliferative response induced by recombinant IL‐2. We found similarities in the tissue distribution and function of the human CD24LAP molecule and the murine, heat‐stable antigen, which suggests that CD24 might function as a signaling molecule on human T cells.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0741-5400 1938-3673
DOI:	10.1189/jlb.69.2.215