Investigation of the Multixenobiotic Resistance Mechanism in the Freshwater Fishes Western Mosquitofish, Gambusia affinis, and Bluegill Sunfish, Lepomis macrochirus

The purpose of the study was to evaluate multixenobiotic resistance mechanism expression as a biomarker for contaminant exposure in freshwater fishes. Exposure to a known mammalian inhibitor (verapamil, 10 μM) and inducer (rhodamine 123, 3 μM) on the transport protein P-glycoprotein (Pgp) was invest...

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Bibliographic Details
Published in:	Bulletin of environmental contamination and toxicology Vol. 83; no. 5; pp. 640 - 643
Main Authors:	Damaré, C. L, Kaddoumi, A. K, Baer, K. N
Format:	Journal Article
Language:	English
Published:	New York New York : Springer-Verlag 01-11-2009 Springer-Verlag Springer Nature B.V
Subjects:	Animals Aquatic Pollution ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism Biomarkers Biomarkers - metabolism Blotting, Western Contaminants Contamination Culicidae Detoxification Earth and Environmental Science Ecotoxicology Environment Environmental Chemistry Environmental Health Fish Fishes Fresh Water Freshwater Freshwater fish Gambusia affinis Gene expression Glycoproteins Lepomis macrochirus Pisces Pollution Rhodamine 123 - pharmacology Soil Science & Conservation Species Specificity Teleostei Verapamil - toxicity Waste Water Technology Water Management Water Pollutants, Chemical - toxicity Water Pollution Control Xenobiotics - toxicity Bluegill sunfish MXR Mosquitofish Glycoprotein
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Summary:	The purpose of the study was to evaluate multixenobiotic resistance mechanism expression as a biomarker for contaminant exposure in freshwater fishes. Exposure to a known mammalian inhibitor (verapamil, 10 μM) and inducer (rhodamine 123, 3 μM) on the transport protein P-glycoprotein (Pgp) was investigated in the liver of the western mosquitofish and bluegill sunfish. No differences in the activity or expression of Pgp were measured in either species using a fluorometric accumulation assay and western blot analyses. The preliminary results from this study indicate that this detoxification mechanism may not be a sensitive indicator of contaminant exposure in certain teleost species.
Bibliography:	http://dx.doi.org/10.1007/s00128-009-9846-x ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0007-4861 1432-0800
DOI:	10.1007/s00128-009-9846-x