Search Results - "Kaback, H R"

It's Better To Be Lucky Than Smart by Kaback, H.R

“…Bacterial cytoplasmic membrane vesicles provide a unique experimental system for studying active transport. Vesicles are prepared by lysis of osmotically…”
Get full text

Toward the Bilayer Proteome, Electrospray Ionization-Mass Spectrometry of Large, Intact Transmembrane Proteins by Whitelegge, J. P., Le Coutre, J., Lee, J. C., Engel, C. K., Prive, G. G., Faull, K. F., Kaback, H. R.

“…Genes encoding membrane proteins comprise a substantial proportion of genomes sequenced to date, but ability to perform structural studies on this portion of…”
Get full text

Structure and Mechanism of the Lactose Permease of Escherichia coli by Abramson, Jeff, Smirnova, Irina, Kasho, Vladimir, Verner, Gillian, Kaback, H. Ronald, Iwata, So

“…Membrane transport proteins that transduce free energy stored in electrochemical ion gradients into a concentration gradient are a major class of membrane…”
Get full text

Functional Interactions between Putative Intramembrane Charged Residues in the Lactose Permease of Escherichia coli by Sahin-Toth, Miklos, Dunten, Rhonda L., Gonzalez, Alberto, Kaback, H. Ronald

“…Using a lactose permease mutant devoid of Cys residues ("C-less permease"), we systematically replaced putative intramembrane charged residues with Cys…”
Get full text

Site-Directed Sulfhydryl Labeling of the Lactose Permease of Escherichia coli:  Helices IV and V That Contain the Major Determinants for Substrate Binding by Kwaw, Isidore, Zen, Kuo-Chang, Hu, Yonglin, Kaback, H. Ronald

“…Helices IV and V in the lactose permease of Escherichia coli contain the major determinants for substrate binding [Glu126 (helix IV), Arg144 (helix V), and…”
Get full text

In vivo Expression of the lacY Gene in Two Segments Leads to Functional lac Permease by Bibi, Eitan, Kaback, H. Ronald

“…The lacY gene of Escherichia coli was cut into two approximately equal-size fragments with Afl II and subcloned individually or together under separate lac…”
Get full text

Cloning and functional expression in bacteria of a novel glucose transporter present in liver, intestine, kidney, and beta-pancreatic islet cells by Thorens, B, Sarkar, H K, Kaback, H R, Lodish, H F

“…The well-characterized erythrocyte glucose transporter is also expressed in brain, adipocytes, kidney, muscle, and certain transformed cells, but not in liver,…”
Get more information

The C-4 Hydroxyl Group of Galactopyranosides Is the Major Determinant for Ligand Recognition by the Lactose Permease of Escherichia coli by Sahin-Tóth, Miklós, Lawrence, Mary C, Nishio, Toshiyuki, Kaback, H. Ronald

“…Binding specificity in lactose permease toward galactopyranosides is governed by H-bonding interactions at C-2, C-3, C-4, and C-6 OH groups, while binding…”
Get full text

Site-Directed Sulfhydryl Labeling of the Lactose Permease of Escherichia coli:  N-Ethylmaleimide-Sensitive Face of Helix II by Venkatesan, Pushpa, Liu, Zhilin, Hu, Yonglin, Kaback, H. Ronald

“…Cys-scanning mutagenesis of helix II in the lactose permease of Escherichia coli [Frillingos, S., Sun, J. et al. (1997) Biochemistry 36, 269−273] indicates…”
Get full text

Properties and Purification of an Active Biotinylated Lactose Permease from Escherichia coli by Consler, Thomas G., Persson, Bengt L., Jung, Heinrich, Zen, Kevin H., Jung, Kirsten, Prive, Gilbert G., Verner, Gillian E., Kaback, H. Ronald

“…A simplified approach for purification of functional lactose permease from Escherichia coli is described that is based on the construction of chimeras between…”
Get full text

Site-Directed Sulfhydryl Labeling of the Lactose Permease of Escherichia coli:  Helix VII by Venkatesan, Pushpa, Kwaw, Isidore, Hu, Yonglin, Kaback, H. Ronald

“…Site-directed sulfhydryl modification in situ is employed to investigate structural and dynamic features of transmembrane helix VII and the beginning of the…”
Get full text

Site-Directed Sulfhydryl Labeling of the Lactose Permease of Escherichia coli:  Helix X by Venkatesan, Pushpa, Hu, Yonglin, Kaback, H. Ronald

“…Helix X in the lactose permease of Escherichia coli contains two residues that are irreplaceable with respect to active transport, His322 and Glu325, as well…”
Get full text

Functional Expression of Mouse mdr1 in Escherichia coli by EITAN BIBI, GROS, P, KABACK, H. R

“…We describe functional expression of the mouse multidrug-resistance protein (P-glycoprotein; P-gp) in an Escherichia coli mutant defective in the outer…”
Get full text

Effect of the Lipid Phase Transition on the Lactose Permease from Escherichia coli by Zhang, Wei, Kaback, H. Ronald

“…The temperature dependence of lactose active transport, efflux down a concentration gradient, and equilibrium exchange were analyzed in right-side-out membrane…”
Get full text

Helix Packing of Lactose Permease in Escherichia coli Studied by Site- Directed Chemical Cleavage by Wu, Jianhua, Perrin, David M., Sigman, David S., Kaback, H. Ronald

“…Biotinylated lactose permease from Escherichia coli containing a single-cysteine residue at position 330 (helix X) or at position 147, 148, or 149 (helix V)…”
Get full text

Use of site-directed fluorescence labeling to study proximity relationships in the lactose permease of Escherichia coli by Jung, Kirsten, Jung, Heinrich, Wu, Jianhua, Prive, Gilbert G, Kaback, H. Ronald

“…The lactose permease of Escherichia coli is a paradigm for polytopic membrane transport proteins that transduce free energy stored in an electrochemical ion…”
Get full text

A Method for Distance Determination in Proteins Using a Designed Metal Ion Binding Site and Site-Directed Spin Labeling: Evaluation with T4 Lysozyme by Voss, J, Salwiński, L, Kaback, H R, Hubbell, W L

“…The use of molecular genetics to introduce both a metal ion binding site and a nitroxide spin label into the same protein opens the use of paramagnetic…”
Get full text

Engineering Conformational Flexibility in the Lactose Permease of Escherichia coli:  Use of Glycine-Scanning Mutagenesis To Rescue Mutant Glu325→Asp by Weinglass, Adam B, Smirnova, Irina N, Kaback, H. Ronald

“…Lactose/H+ symport by lactose permease of Escherichia coli involves interactions between four irreplaceable charged residues in transmembrane helices that play…”
Get full text

Insertional Mutagenesis of Hydrophilic Domains in the Lactose Permease of Escherichia coli by McKenna, Edward, Hardy, Dorothy, Kaback, H. Ronald

“…The lactose permease of Escherichia coli is a membrane transport protein postulated to contain a hydrophilic N terminus (hydrophilic domain 1), 12 hydrophobic…”
Get full text

The kamikaze approach to membrane transport by Kaback, H. Ronald, Sahin-Tóth, Miklós, Weinglass, Adam B

“…Membrane transport proteins catalyse the movement of molecules into and out of cells and organelles, but their hydrophobic and metastable nature often makes…”
Get full text