Paraoxonase and arylesterase activity of paraoxonase 1 and oxidative stress parameters in cervical intraepithelial neoplasia
Paraoxonase 1 (PON1) is the enzyme that removes carcinogenic radicals from lipids. The aim of the study was to investigate the differences in PON1 activity and oxidation stress parameters between patients with cervical intraepithelial neoplasia (CIN) and healthy controls. The study included 65 women...
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| Published in: | Biochemia Medica Vol. 34; no. 1; p. 030701 |
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| Main Authors: | , , , , , , , , |
| Format: | Journal Article Paper |
| Language: | English |
| Published: |
Croatia
Medicinska naklada
15-02-2024
Croatian Society of Medical Biochemistry and Laboratory Medicine |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Paraoxonase 1 (PON1) is the enzyme that removes carcinogenic radicals from lipids. The aim of the study was to investigate the differences in PON1 activity and oxidation stress parameters between patients with cervical intraepithelial neoplasia (CIN) and healthy controls.
The study included 65 women with CIN and 109 healthy women. Lipid parameters were determined on Cobas Integra 400 plus (Roche, Mannheim, Germany). Tiols and reduced glutathione (GSH) were determined spectrophotometric using Eliman reagent. Activity of PON1 was assessed with two substrates, paraoxon and phenylacetate by spectrophotometric method. Malondialdehyde (MDA) was determined by high performance liquid chromatography (Shimadzu Corporation, Kyoto, Japan). Mann-Whitney-test, t-test, χ2-test, correlation and logistic regression was used in statistical analysis. P < 0.05 was considered statistically significant.
The basal (P = 0.929) and NaCl-stimulated (P = 0.985) PON1 activity and activities standardised on the concentration of high-density lipoprotein (HDL; P = 0.076; P = 0.065, respectively) and apolipoprotein AI (apo AI; P = 0.444; P = 0.499, respectively) as well as PON1 phenotypes (P = 0.842) did not differ significantly between the groups. The PON1 arylesterase activity (53±19 kU/L vs. 77±17 kU/L; P < 0.001) and HDL-standardized activity (37 (28-44) kU/mmol
. 43 (37-50) kU/mmol; P < 0.001) and apoAI (29±11 kU/g
. 44±11 kU/g; P < 0.001) was significantly reduced in the CIN group. The concentration of the thiol groups was similar (P = 0.519), of MDA was lower (0.39 (0.27-0.55) µmol/L
. 0.76 (0.57-1.15) µmol/L; P < 0.001) and of GSH was higher (112.0 (66.0-129.6) µg/mL
. 53.4 (34.8-134.4) µg/mL; P < 0.001) in the CIN group.
Reduced PON1 arylesterase activity, lower MDA and higher GSH concentration were observed in CIN patients. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 312202 D Butorac: Conceptualization, Data duration, Investigation, Writing - original draft; I Ćelap: Investigation, Writing - original draft; S Kačkov Maslač: Investigation; T Miletić: Data duration; A Hulina Tomašković: Investigation; P Turčić: Investigation; D Rašić: Investigation; I Stojanović: Data duration; M Grdić Rajković: Conceptualization, Project administration, Supervision, Visualisation; Writing - review & editing. Author contributions |
| ISSN: | 1330-0962 1846-7482 1846-7482 |
| DOI: | 10.11613/BM.2024.010701 |