Search Results - "KWAN, Emily M"

N-Glycosidase–carbohydrate-binding module fusion proteins as immobilized enzymes for protein deglycosylation by Kwan, Emily M., Boraston, Alisdair B., McLean, Bradley W., Kilburn, Douglas G., Warren, R. Antony J.

“…A carbohydrate-binding module (CBM) was fused to the N-termini of mannosyl-glycoprotein endo-β-N-acetylglucosaminidase (EndoF1) and peptide N-glycosidase F…”
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Glycosynthase-Mediated Assembly of Xylanase Substrates and Inhibitors by Goddard-Borger, Ethan D., Fiege, Brigitte, Kwan, Emily M., Withers, Stephen G.

“…An exo‐β‐xylosidase mutant with glycosynthase activity was created to aid in the synthesis of xylanase substrates and inhibitors. Simple monosaccharides were…”
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Probing Electrostatic Interactions along the Reaction Pathway of a Glycoside Hydrolase:  Histidine Characterization by NMR Spectroscopy by Schubert, Mario, Poon, David K. Y, Wicki, Jacqueline, Tarling, Chris A, Kwan, Emily M, Nielsen, Jens E, Withers, Stephen G, McIntosh, Lawrence P

“…We have characterized by NMR spectroscopy the three active site (His80, His85, and His205) and two non-active site (His107 and His114) histidines in the 34 kDa…”
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NMR Spectroscopic Characterization of a β-(1,4)-Glycosidase along Its Reaction Pathway:  Stabilization upon Formation of the Glycosyl−Enzyme Intermediate by Poon, David K. Y, Ludwiczek, Martin L, Schubert, Mario, Kwan, Emily M, Withers, Stephen G, McIntosh, Lawrence P

“…NMR spectroscopy was used to search for mechanistically significant differences between the thermodynamic and dynamic properties of the 34 kDa (α/β)8-barrel…”
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Production of a self-activating CBM-factor X fusion protein in a stable transformed Sf9 insect cell line using high cell density perfusion culture by GORENFLO, Volker M, PFEIFER, Tom A, LESNICKI, Gary, KWAN, Emily M, GRIGLIATTI, Thomas A, KILBURN, Douglas G, PIRET, James M

“…Factor Xa is a serine protease, whose high selectivity can be used to cleave protein tags from recombinant proteins. A fusion protein comprised of a…”
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