Transfer of amiprophosmethyl resistance from a Nicotiana plumbaginifolia mutant by somatic hybridisation

Transfer of resistance to the phosphorothioamidate herbicide, amiprophosmethyl (APM), from the β-tubulin mutant of Nicotiana plumbaginifolia to the interspecific N. plumbaginifolia (+) N. sylvestris and to the intertribal N. plumbaginifolia (+) Atropa belladonna somatic hybrids has been demonstrated...

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Published in:	Theoretical and applied genetics Vol. 100; no. 6; pp. 847 - 857
Main Authors:	YEMETS, A. I, KUNDEL'CHUK, O. P, SMERTENKO, A. P, SOLODUSHKO, V. G, RUDAS, V. A, GLEBA, Y. Y, BLUME, Y. B
Format:	Journal Article
Language:	English
Published:	Heidelberg Springer 01-04-2000 Berlin Springer Nature B.V
Subjects:	Agronomy. Soil science and plant productions amiprophospmethyl Atropa belladonna b-tubulin Biological and medical sciences Cloning Fundamental and applied biological sciences. Psychology Genetics and breeding of economic plants Haploidy, in vitro culture applications, somatic hybrids Herbicides Nicotiana plumbaginifolia Nicotiana sylvestris Plant breeding: fundamental aspects and methodology Ukraine Pesticides Chromosome Herbicide Resistance Ionizing irradiation Nicotiana plumbaginifolia Dicotyledones Protoplast Seed Angiospermae Transfer Genetics Somatic hybrid Spermatophyta Cross resistance Trifluralin Solanaceae Mutation Organic thioamidophosphate
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Summary:	Transfer of resistance to the phosphorothioamidate herbicide, amiprophosmethyl (APM), from the β-tubulin mutant of Nicotiana plumbaginifolia to the interspecific N. plumbaginifolia (+) N. sylvestris and to the intertribal N. plumbaginifolia (+) Atropa belladonna somatic hybrids has been demonstrated. Transfer to the recipient species was accomplished by: (1) symmetric hybridisationand (2) asymmetric hybridisation using γ-irradiation of donor protoplasts. Cytogenetic analysis confirmed the hybrid origin of the hybrids obtained. It was established that most of them typically inherited no more than three donor chromosomes, although it was possible to obtain symmetric hybrids in the case of symmetric fusion. Immunofluorescent microscopy analysis has shown that protoplasts of the mutant, and of the N. plumbaginifolia (+) N. sylvestris and N. plumbaginifolia (+) A. belladonna hybrids, retained the normal structure of interphase microtubule (MT) arrays and mitotic figures after treatment with 5 µM APM, whereas MTs of protoplasts of the recipients were destroyed under these conditions. It was also shown that hybrid clones contained an altered β-tubulin isoform originating from the N. plumbaginifolia mutant. The selected hybrid clones were characterised by cross-resistance to trifluralin, a dinitroaniline herbicide with the same mode of anti-MT action. Some of the somatic hybrids which could flower were fertile. It was established that seeds of some fertile hybrids were able to germinate in the presence of 5 µM APM. The results obtained thus support the conclusion that the technique of somatic hybridisation, especially asymmetric fusion, can be used to transfer APM resistance from the N. plumbaginifolia mutant to different (related and remote) plant species of the Solanaceae, including important crops.[PUBLICATION ABSTRACT]
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0040-5752 1432-2242
DOI:	10.1007/s001220051361