Search Results - "KULOMAA, Markku S"
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Biochemical Characterization of CA IX, One of the Most Active Carbonic Anhydrase Isozymes
Published in The Journal of biological chemistry (10-10-2008)“…Carbonic anhydrase IX (CA IX) is an exceptional member of the CA protein family; in addition to its classical role in pH regulation, it has also been proposed…”
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Molecular features of steroid-binding antidins and their use for assaying serum progesterone
Published in PloS one (20-02-2019)“…Chicken avidin (Avd) and streptavidin from Streptomyces avidinii are extensively used in bionanotechnology due to their extremely tight binding to biotin (Kd ~…”
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Structural characterization of core-bradavidin in complex with biotin
Published in PloS one (20-04-2017)“…Bradavidin is a tetrameric biotin-binding protein similar to chicken avidin and bacterial streptavidin, and was originally cloned from the nitrogen-fixing…”
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Rhizavidin from Rhizobium etli: the first natural dimer in the avidin protein family
Published in Biochemical journal (01-08-2007)“…Rhizobium etli CFN42 is a symbiotic nitrogen-fixing bacterium of the common bean Phaseolus vulgaris. The symbiotic plasmid p42d of R. etli comprises a gene…”
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A novel chimeric avidin with increased thermal stability using DNA shuffling
Published in PloS one (14-03-2014)“…Avidins are a family of proteins widely employed in biotechnology. We have previously shown that functional chimeric mutant proteins can be created from avidin…”
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Zebavidin--an avidin-like protein from zebrafish
Published in PloS one (24-10-2013)“…The avidin protein family members are well known for their high affinity towards D-biotin and high structural stability. These properties make avidins valuable…”
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Purification of norovirus-like particles (VLPs) by ion exchange chromatography
Published in Journal of virological methods (01-04-2012)“…► Scalable purification method for norovirus VLPs based on anion exchange chromatography. ► High particle purity obtained. ► Purified VLPs were stable for over…”
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Production and characterization of virus-like particles and the P domain protein of GII.4 norovirus
Published in Journal of virological methods (01-01-2012)“…Noroviruses are an important cause of epidemic acute gastroenteritis in humans. In this study the production and characterization of GII.4 norovirus virus-like…”
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Chimeric Avidin--NMR structure and dynamics of a 56 kDa homotetrameric thermostable protein
Published in PloS one (24-06-2014)“…Chimeric avidin (ChiAVD) is a product of rational protein engineering remarkably resistant to heat and harsh conditions. In quest of the fundamentals behind…”
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10
Structure of bradavidin-C-terminal residues act as intrinsic ligands
Published in PloS one (04-05-2012)“…Bradavidin is a homotetrameric biotin-binding protein from Bradyrhizobium japonicum, a nitrogen fixing and root nodule-forming symbiotic bacterium of the…”
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Bifunctional avidin with covalently modifiable ligand binding site
Published in PloS one (27-01-2011)“…The extensive use of avidin and streptavidin in life sciences originates from the extraordinary tight biotin-binding affinity of these tetrameric proteins…”
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Isolation and Characterization of Novosphingobium sp. Strain MT1, a Dominant Polychlorophenol-Degrading Strain in a Groundwater Bioremediation System
Published in Applied and Environmental Microbiology (01-01-2002)“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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13
Construction of chimeric dual-chain avidin by tandem fusion of the related avidins
Published in PloS one (31-05-2011)“…Avidin is a chicken egg-white protein with high affinity to vitamin H, also known as D-biotin. Many applications in life science research are based on this…”
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Internalization of novel non-viral vector TAT-streptavidin into human cells
Published in BMC biotechnology (2007)“…The cell-penetrating peptide derived from the Human immunodeficiency virus-1 transactivator protein Tat possesses the capacity to promote the effective uptake…”
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Tetravalent single-chain avidin: from subunits to protein domains via circularly permuted avidins
Published in Biochemical journal (15-12-2005)“…scAvd (single-chain avidin, where two dcAvd are joined in a single polypeptide chain), having four biotin-binding domains, was constructed by fusion of…”
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Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli
Published in Biochemical journal (01-12-2004)“…Chicken avidin is a highly popular tool with countless applications in the life sciences. In the present study, an efficient method for producing avidin…”
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17
Evidence for Natural Horizontal Transfer of the pcpB Gene in the Evolution of Polychlorophenol-Degrading Sphingomonads
Published in Applied and Environmental Microbiology (01-09-2002)“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Improvement in Nuclear Entry and Transgene Expression of Baculoviruses by Disintegration of Microtubules in Human Hepatocytes
Published in Journal of Virology (01-03-2005)“…Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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19
Design and Construction of Highly Stable, Protease-resistant Chimeric Avidins
Published in The Journal of biological chemistry (18-03-2005)“…The chicken avidin gene family consists of avidin and seven separate avidin-related genes (AVRs) 1–7. Avidin protein is a widely used biochemical tool, whereas…”
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20
Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein
Published in BMC biotechnology (09-06-2011)“…Engineered proteins, with non-immunoglobulin scaffolds, have become an important alternative to antibodies in many biotechnical and therapeutic applications…”
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