Diagnostic accuracy of a serotype-specific antigen test in community-acquired pneumonia

Diagnostic accuracy of a serotype-specific antigen test in community-acquired pneumonia

Our aim was to evaluate the diagnostic accuracy and clinical utility of a serotype-specific urinary antigen detection multiplex assay for identification of 13 pneumococcal serotypes (1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F and 23F) in urine of patients with community-acquired pneumonia. Adult...

Full description

Saved in:
Bibliographic Details
Published in:The European respiratory journal Vol. 42; no. 5; pp. 1283 - 1290
Main Authors: HUIJTS, Susanne M, PRIDE, Michael W, KUIPERS, Bart A. F, VAN DER ENDE, Arie, BONTEN, Marc J. M, VOS, Josephine M. I, JANSEN, Kathrin U, WEBBER, Chris, GRUBER, William, BOERSMA, Wim G, SNIJDERS, Dominic, KLUYTMANS, Jan A. J. W, VAN DER LEE, Ivo
Format: Journal Article
Language:English
Published: Leeds Maney 01-11-2013
Subjects:
Aged
Antigens, Bacterial - urine
Biological and medical sciences
Community-Acquired Infections - diagnosis
Community-Acquired Infections - immunology
Community-Acquired Infections - urine
Female
Humans
Male
Medical sciences
Middle Aged
Pneumology
Pneumonia, Pneumococcal - diagnosis
Pneumonia, Pneumococcal - immunology
Pneumonia, Pneumococcal - urine
Polysaccharides - analysis
Prevalence
Prospective Studies
Reproducibility of Results
Sensitivity and Specificity
Antigen
Lung disease
Pneumonia
Accuracy
Community acquired infection
Typing
Respiratory disease
Diagnosis
Serotype
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Our aim was to evaluate the diagnostic accuracy and clinical utility of a serotype-specific urinary antigen detection multiplex assay for identification of 13 pneumococcal serotypes (1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F and 23F) in urine of patients with community-acquired pneumonia. Adult patients with clinical suspicion of community-acquired pneumonia were included. In addition to standard diagnostic procedures, a urine sample was collected to perform the urinary antigen detection test. Demographic, clinical, radiological and microbiological data were collected. Among 1095 community-acquired pneumonia patients Streptococcus pneumoniae was identified as causative pathogen in 257 (23%), when using conventional diagnostic methods and in 357 (33%) when urinary antigen detection was added. Of the 49 bacteraemic episodes caused by one of the 13 serotypes covered by the urinary antigen detection, 48 were detected by the urinary antigen detection, indicating a sensitivity of 98%. Of the 77 community-acquired pneumonia episodes with a "non-urinary antigen detection" causative pathogen, none had a positive urinary antigen detection result, indicating a specificity of 100%. Addition of the urinary antigen detection test to conventional diagnostic methods increased the prevalence of S. pneumoniae community-acquired pneumonia by 39%. Using bacteraemic episodes as reference sensitivity and specificity of the urinary antigen detection was 98% and 100%, respectively.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Undefined-2
ISSN:0903-1936
1399-3003
DOI:10.1183/09031936.00137412