Abnormal Increase in the Expression Level of Proliferating Cell Nuclear Antigen (PCNA) in the Liver and Hepatic Injury in Rats with Dietary Cobalamin Deficiency

Dietary cobalamin (Cbl; vitamin B12) deficiency resulted in severe growth retardation in rats, and body weight in the Cbl-deficient rats at 20 wk of age was significantly lower compared with the age-matched Cbl-sufficient control rats. In contrast, liver weight, when normalized to body weight, was g...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of Nutritional Science and Vitaminology Vol. 52; no. 3; pp. 168 - 173
Main Authors:	NAKAO, Motoyuki, KONO, Nozomi, ADACHI, Satoko, EBARA, Shuhei, ADACHI, Tetsuya, MIURA, Takumi, YAMAJI, Ryoichi, INUI, Hiroshi, NAKANO, Yoshihisa
Format:	Journal Article
Language:	English
Published:	Tokyo Center for Academic Publications Japan 01-06-2006
Subjects:	Alanine Transaminase - blood Animals Biological and medical sciences Biomarkers - metabolism Body Weight - physiology Carnitine - therapeutic use Cell Proliferation cobalamin-deficient rats Feeding. Feeding behavior Fundamental and applied biological sciences. Psychology Growth Disorders - etiology Liver - metabolism Liver Diseases - blood Liver Diseases - etiology liver injury Male Methionine - therapeutic use methionine synthase Methylmalonic Acid - urine methylmalonyl-CoA mutase Organ Size - physiology proliferating cell nuclear antigen Proliferating Cell Nuclear Antigen - metabolism Rats Rats, Wistar Vertebrates: anatomy and physiology, studies on body, several organs or systems Vitamin B 12 Deficiency - complications Vitamin B 12 Deficiency - diet therapy Vitamin B Complex - therapeutic use O-Acetylhomoserine (thiol)-lyase liver injury Rat Digestive system Enzyme Injury Deficiency Liver methionine synthase Rodentia Lyases Gene expression proliferating cell nuclear antigen Vertebrata Isomerases Mammalia Animal Intramolecular transferases cobalamin-deficient rats Lesion Carbon-oxygen lyases PCNA antigen Methylmalonyl-CoA mutase
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Dietary cobalamin (Cbl; vitamin B12) deficiency resulted in severe growth retardation in rats, and body weight in the Cbl-deficient rats at 20 wk of age was significantly lower compared with the age-matched Cbl-sufficient control rats. In contrast, liver weight, when normalized to body weight, was greater in the Cbl-deficient rats than in the controls (p<0.05). The expression level of proliferating cell nuclear antigen (PCNA), a marker for cell proliferation, in the liver was significantly enhanced in the deficient rats, suggesting that cell proliferation is abnormally activated in the liver under Cbl-deficient conditions. In addition, plasma alanine aminotransferase (ALT) activity, a marker for hepatic injury, was also significantly elevated in the deficient rats. When L-carnitine, which is used clinically for the treatment of Cbl-deficient patients with methylmalonic aciduria, was administered to the Cbl-deficient rats by intraperitoneal injection twice per day for 2 wk (each 0.5 mmol), the amount of methylmalonic acid excreted into the urine was significantly reduced, and the plasma ALT activity was lowered to a normal level. However, the PCNA expression in the liver was barely influenced by the treatment with carnitine. In contrast, when the deficient rats were fed an L-methionine-supplemented diet (4 g of L-methionine per kg of the diet) for 2 wk, the increased expression of PCNA was normalized.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0301-4800 1881-7742
DOI:	10.3177/jnsv.52.168