Search Results - "KLEINFELD, A. M"

Unbound free fatty acid levels in human serum by Richieri, G V, Kleinfeld, A M

“…Total serum free fatty acids (FFAt) levels provide an important measure of the physiologic state. Most of the FFA in serum is bound to albumin; a small…”
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A fluorescently labeled intestinal fatty acid binding protein. Interactions with fatty acids and its use in monitoring free fatty acids by RICHIERI, G. V, OGATA, R. T, KLEINFELD, A. M

“…The fatty acid-binding protein from rat intestine (I-FABP) has been covalently modified with the fluorescent compound Acrylodan. Acrylodan was found to label…”
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Interactions of long-chain fatty acids and albumin: Determination of free fatty acid levels using the fluorescent probe ADIFAB by Richieri, Gary V, Anel, Alberto, Kleinfeld, Alan M

“…Equilibrium binding of long-chain fatty acids (FA) with albumin from human serum (HSA), bovine serum (BSA), and murine serum (MSA) has been studied by…”
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Equilibrium constants for the binding of fatty acids with fatty acid-binding proteins from adipocyte, intestine, heart, and liver measured with the fluorescent probe ADIFAB by Richieri, G V, Ogata, R T, Kleinfeld, A M

“…Affinities of long chain fatty acids (FA) for fatty acid-binding proteins (FABPs) have been measured by monitoring the concentrations of the unbound or free…”
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Lipid phase fatty acid flip-flop, is it fast enough for cellular transport? by Kleinfeld, A M

“…The mechanism by which fatty acids are transported across cell membranes is controversial. The essence of the controversy is whether transport requires…”
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The measurement of free fatty acid concentration with the fluorescent probe ADIFAB: a practical guide for the use of the ADIFAB probe by Richieri, G V, Ogata, R T, Kleinfeld, A M

“…The aqueous phase monomers of fatty acids (FFA) appear in many steps of fat metabolism. Understanding metabolism requires that accurate measurements of FFA…”
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Membrane partition of fatty acids and inhibition of T cell function by Anel, Alberto, Richieri, Gary V, Kleinfeld, Alan M

“…Short-term exposure to elevated levels of free fatty acids (FFA) perturbs a variety of cellular functions. It is frequently observed that cis-unsaturated fatty…”
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Increases in serum unbound free fatty acid levels following coronary angioplasty by Kleinfeld, Alan M., Prothro, David, Brown, David L., Davis, Robert C., Richieri, Gary V., DeMaria, Anthony

“…Serum unbound free fatty acid levels (FFA u) were measured in patients undergoing percutaneous transluminal coronary angioplasty (PTCA) using the fluorescent…”
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Transfer of long-chain fluorescent fatty acids between small and large unilamellar vesicles by Kleinfeld, Alan M, Storch, Judith

“…Transfer of 12-(9-anthroyloxy)stearic acid (12AS) was measured between small unilamellar vesicles (SUV) and between large unilamellar vesicles (LUV), over a…”
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Studies of the Fatty Acid-binding Site of Rat Liver Fatty Acid-binding Protein Using Fluorescent Fatty Acids by Storch, J, Bass, N M, Kleinfeld, A M

“…Rat liver fatty acid-binding protein (FABP) is a 14.3-kDa cytosolic protein which binds long chain free fatty acids (ffa) and is believed to participate in…”
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Direct determination of free fatty acid transport across the adipocyte plasma membrane using quantitative fluorescence microscopy by STORCH, J, LECHENE, C, KLEINFELD, A. M

“…Movement of free fatty acids (FFA) across the plasma membrane has been directly measured for the first time, using fluorescent FFA analogs and quantitative…”
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A tyrosine phosphorylation requirement for cytotoxic T lymphocyte degranulation by ANEL, A, RICHIERI, G. V, KLEINFELD, A. M

“…Phorbol myristate acetate (PMA) plus ionomycin induces the tyrosine phosphorylation of several cytotoxic T lymphocyte (CTL) substrates, including one with an…”
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Tyrosine phosphorylation of a 100-kDa protein is correlated with cytotoxic T-lymphocyte function. Evidence from cis unsaturated fatty acid and phenylarsineoxide inhibition by ANEL, A, KLEINFELD, A. M

“…Cis unsaturated but not saturated fatty acids (FA) have been shown to specifically inhibit certain early responses in cytotoxic T-lymphocytes (CTL) including…”
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Transfer of long-chain fluorescent free fatty acids between unilamellar vesicles by Storch, Judith, Kleinfeld, Alan M

“…Movement of free fatty acids (ffa) between small unilamellar vesicles (SUV) was studied by measuring the transfer of fluorescent n-(9-anthroyloxy)-labeled…”
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Plasma membrane lipid order and composition during adipocyte differentiation of 3T3F442A cells. Studies in intact cells with 1-[4-(trimethylamino)phenyl]-6-phenylhexatriene by STORCH, J, SHULMAN, S. L, KLEINFELD, A. M

“…The plasma membrane lipid order of 3T3F442A cells was examined during the course of adipocyte differentiation by measuring the fluorescence polarization of…”
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Fatty Acid Binding Proteins from Different Tissues Show Distinct Patterns of Fatty Acid Interactions by Richieri, Gary V, Ogata, Ronald T, Zimmerman, Aukje W, Veerkamp, Jacques H, Kleinfeld, Alan M

“…Fatty acid binding proteins (FABP) form a family of proteins displaying tissue-specific expression. These proteins are involved in fatty acid (FA) transport…”
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Free fatty acid perturbation of transmembrane signaling in cytotoxic T lymphocytes by Richieri, GV, Kleinfeld, AM

“…Transmembrane signaling in CTL is found to be extremely sensitive to short term exposure to long chain free fatty acids (FFA). Both alloantigen specific target…”
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Lipid domains in membranes. Evidence derived from structural perturbations induced by free fatty acids and lifetime heterogeneity analysis by Klausner, R D, Kleinfeld, A M, Hoover, R L, Karnovsky, M J

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Partition of fatty acids and fluorescent fatty acids into membranes by Pjura, W. J, Kleinfeld, A. M, Karnovsky, M. J

“…We have measured the membrane/water partition of free fatty acids, of a fluorescent analogue, and of chlorpromazine into plasma membranes from lymphoma,…”
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Free fatty acids inhibit cytotoxic T lymphocyte-mediated lysis of allogeneic target cells by Richieri, GV, Kleinfeld, AM

“…Short term exposure of murine CTL clones to long chain cis unsaturated free fatty acid (FFA) inhibits alloantigen specific lysis of cognate target cells,…”
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