Search Results - "KINCAID, James R."

Unveiling the crucial intermediates in androgen production by Mak, Piotr J., Gregory, Michael C., Denisov, Ilia G., Sligar, Stephen G., Kincaid, James R.

“…Ablation of androgen production through surgery is one strategy against prostate cancer, with the current focus placed on pharmaceutical intervention to…”
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Resonance Raman Spectroscopy of the Oxygenated Intermediates of Human CYP19A1 Implicates a Compound I Intermediate in the Final Lyase Step by Mak, Piotr J, Luthra, Abhinav, Sligar, Stephen G, Kincaid, James R

“…CYP19A1, or aromatase, a cytochrome P450 responsible for estrogen biosynthesis in humans, is an important therapeutic target for the treatment of breast…”
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Evidence that cytochrome b5 acts as a redox donor in CYP17A1 mediated androgen synthesis by Duggal, Ruchia, Liu, Yilin, Gregory, Michael C., Denisov, Ilia G., Kincaid, James R., Sligar, Stephen G.

“…Cytochrome P450 17A1 (CYP17A1) is an important drug target for castration resistant prostate cancer. It is a bi-functional enzyme, catalyzing production of…”
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Differential Control of Heme Reactivity in Alpha and Beta Subunits of Hemoglobin: A Combined Raman Spectroscopic and Computational Study by Jones, Eric M, Monza, Emanuele, Balakrishnan, Gurusamy, Blouin, George C, Mak, Piotr J, Zhu, Qianhong, Kincaid, James R, Guallar, Victor, Spiro, Thomas G

“…The use of hybrid hemoglobin (Hb), with mesoheme substituted for protoheme, allows separate monitoring of the α or β hemes along the allosteric pathway. Using…”
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Resonance Raman Characterization of the Peroxo and Hydroperoxo Intermediates in Cytochrome P450 by Denisov, Ilia G, Mak, Piotr J, Makris, Thomas M, Sligar, Stephen G, Kincaid, James R

“…Resonance Raman (RR) studies of intermediates generated by cryoreduction of the oxyferrous complex of the D251N mutant of cytochrome P450cam (CYP101) are…”
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Analysis of Heme Iron Coordination in DGCR8: The Heme-Binding Component of the Microprocessor Complex by Girvan, Hazel M, Bradley, Justin M, Cheesman, Myles R, Kincaid, James R, Liu, Yilin, Czarnecki, Kazimierz, Fisher, Karl, Leys, David, Rigby, Stephen E. J, Munro, Andrew W

“…DGCR8 is the RNA-binding partner of the nuclease Drosha. Their complex (the “Microprocessor”) is essential for processing of long, primary microRNAs…”
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Defining CYP3A4 Structural Responses to Substrate Binding. Raman Spectroscopic Studies of a Nanodisc-Incorporated Mammalian Cytochrome P450 by Mak, Piotr J, Denisov, Ilia G, Grinkova, Yelena V, Sligar, Stephen G, Kincaid, James R

“…Resonance Raman (RR) spectroscopy is used to help define active site structural responses of nanodisc-incorporated CYP3A4 to the binding of three substrates:…”
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Resonance Raman determination of vinyl group disposition in different derivatives of native myoglobin and its heme-disoriented form by Rwere, Freeborn, Mak, Piotr J., Kincaid, James R.

“…In heme‐reconstituted heme proteins, the heme inserts such that some of the heme is rotated 180° about the α–γ meso axis, eventually equilibrating to native…”
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Resonance Raman studies of gas sensing heme proteins by Liu, Yilin, Kincaid, James R.

“…Heme sensor proteins are present in organisms ranging from bacteria to mammals, typically consisting of a catalytic domain and a heme‐containing sensor domain,…”
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Resonance Raman Interrogation of the Consequences of Heme Rotational Disorder in Myoglobin and Its Ligated Derivatives by Rwere, Freeborn, Mak, Piotr J, Kincaid, James R

“…Resonance Raman spectroscopy is employed to characterize heme site structural changes arising from conformational heterogeneity in deoxyMb and ligated…”
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Resonance Raman Detection of the Hydroperoxo Intermediate in the Cytochrome P450 Enzymatic Cycle by Mak, Piotr J, Denisov, Ilia G, Victoria, Doreen, Makris, Thomas M, Deng, Tianjing, Sligar, Stephen G, Kincaid, James R

“…The resonance Raman spectra of the hydroperoxo complex of camphor-bound CYP101 have been obtained by cryoradiolytic reduction of the oxygenated ferrous form…”
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Revealing substrate-induced structural changes in active site of human CYP51 in the presence of its physiological substrates by Jing, Yuanqi, Usai, Remigio, Liu, Yilin, Kincaid, James R.

“…The human sterol 14α-demethylases (CYP51, CYP is an abbreviation for cytochrome P450) catalyze three-step oxidative removal of 14α-methyl group of lanosterol…”
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Cryoradiolysis of oxygenated cytochrome P450 17A1 with lyase substrates generates expected products by Usai, Remigio, Denisov, Ilia G., Sligar, Stephen G., Kincaid, James R.

“…When subjected to γ-irradiation at cryogenic temperatures the oxygenated complexes of Cytochrome P450 CYP17A1 (CYP17A1) bound with either of the lyase…”
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Importance of Asparagine 202 in Manipulating Active Site Structure and Substrate Preference for Human CYP17A1 by Liu, Yilin, Denisov, Ilia, Gregory, Michael, Sligar, Stephen G, Kincaid, James R

“…The multifunctional cytochrome P450 17A1 (CYP17A1) plays a crucial role in human steroid hormone synthesis (UniProtKBP05093). It first carries out standard…”
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Substrate-Specific Allosteric Effects on the Enhancement of CYP17A1 Lyase Efficiency by Cytochrome b5 by Liu, Yilin, Denisov, Ilia G, Sligar, Stephen G, Kincaid, James R

“…CYP17A1 is an essential human steroidogenic enzyme, which catalyzes two sequential reactions leading to the formation of androstenedione from progesterone and…”
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Solution phase refinement of active site structure using 2D NMR and judiciously 13C-labeled cytochrome P450 by Usai, Remigio, Kaluka, Daniel, Cai, Sheng, Sem, Daniel S., Kincaid, James R.

“…The Cytochrome P450 (CYP450) superfamily has been the subject of intense research for over six decades. Here the HU227 strain of E. coli, lacking the…”
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Subunit-Selective Interrogation of CO Recombination in Carbonmonoxy Hemoglobin by Isotope-Edited Time-Resolved Resonance Raman Spectroscopy by Balakrishnan, Gurusamy, Zhao, Xiaojie, Podstawska, Edyta, Proniewicz, Leonard M, Kincaid, James R, Spiro, Thomas G

“…Hemoglobin (Hb) is an allosteric tetrameric protein made up of αβ heterodimers. The α and β chains are similar, but are chemically and structurally distinct…”
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Substrate-Specific Allosteric Effects on the Enhancement of CYP17A1 Lyase Efficiency by Cytochrome b 5 by Liu, Yilin, Denisov, Ilia G, Sligar, Stephen G, Kincaid, James R

“…CYP17A1 is an essential human steroidogenic enzyme, which catalyzes two sequential reactions leading to the formation of androstenedione from progesterone and…”
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Resonance Raman Spectroscopy Reveals pH-Dependent Active Site Structural Changes of Lactoperoxidase Compound 0 and Its Ferryl Heme O–O Bond Cleavage Products by Mak, Piotr J, Thammawichai, Warut, Wiedenhoeft, Dennis, Kincaid, James R

“…The first step in the enzymatic cycle of mammalian peroxidases, including lactoperoxidase (LPO), is binding of hydrogen peroxide to the ferric resting state to…”
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Mechanism of the Clinically Relevant E305G Mutation in Human P450 CYP17A1 by Liu, Yilin, Grinkova, Yelena, Gregory, Michael C, Denisov, Ilia G, Kincaid, James R, Sligar, Stephen G

“…Steroid metabolism in humans originates from cholesterol and involves several enzyme reactions including dehydrogenation, hydroxylation, and carbon–carbon bond…”
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